Mouse Anti-CHFR Recombinant Antibody (1H3-A12) (CBMAB-C4245-LY)

Name: Mouse Anti-CHFR Recombinant Antibody (1H3-A12)
SKU: CBMAB-C4245-LY
Rating: 5 (1 reviews)

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Published Data

Mouse Anti-CHFR Recombinant Antibody (1H3-A12) (CBMAB-C4245-LY) in Immunoblot

HCT116 and HSC44 cells, which do not express endogenous CHFR, were infected with adenoviruses expressing CHFR (CHFR WT), enhanced green fluorescent protein (EGFP), or LacZ. After infection for 64 h, the cells were treated with 0.5 μg/ml of nocodazole (noc) or DMSO (-) for 27 h. The cell lysates were examined by immunoblotting with the indicated antibodies. ...View More

Kashima, L., Idogawa, M., Mita, H., Shitashige, M., Yamada, T., Ogi, K., ... & Tokino, T. (2012). CHFR protein regulates mitotic checkpoint by targeting PARP-1 protein for ubiquitination and degradation. Journal of Biological Chemistry, 287(16), 12975-12984.

Distributed under Open Access license CC BY 4.0, without modification.

Mouse Anti-CHFR Recombinant Antibody (1H3-A12) (CBMAB-C4245-LY) in Immunoblot and IP

HCT116 cells were mock-transfected or transfected with a FLAG-CHFR expression vector. After transfection for 24 h, the cells were treated for 16 h with 3 mm 3AB or DMSO. The cell lysates were coimmunoprecipitated with an anti-FLAG antibody, and the precipitates were examined by immunoblotting with each of antibodies against the indicated proteins. ...View More

Distributed under Open Access license CC BY 4.0, without modification.

Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

1H3-A12

Application

ELISA, IHC-P, WB

Immunogen

Full length recombinant CHFR.

Host Species

Mouse

Specificity

Human

Antibody Isotype

IgG1, κ

Clonality

Monoclonal Antibody

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Application	Note
IHC-P	3 µg/ml

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS

Preservative

None

Concentration

Batch dependent

Purity

> 95% Purity determined by SDS-PAGE.

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

More Infomation

Target

Full Name

Checkpoint With Forkhead And Ring Finger Domains

Introduction

This gene encodes an E3 ubiquitin-protein ligase required for the maintenance of the antephase checkpoint that regulates cell cycle entry into mitosis and, therefore, may play a key role in cell cycle progression and tumorigenesis. The encoded protein has an N-terminal forkhead-associated domain, a central RING-finger domain, and a cysteine-rich C-terminal region. Alternatively spliced transcript variants that encode different protein isoforms have been described. [provided by RefSeq, Mar 2014]

Entrez Gene ID

55743

UniProt ID

Q96EP1

Alternative Names

Checkpoint With Forkhead And Ring Finger Domains; RING-Type E3 Ubiquitin Transferase CHFR; RING Finger Protein 196; RNF196; Checkpoint With Forkhead And Ring Finger Domains, E3 Ubiquitin Protein Ligase; Checkpoint With Forkhead And RING Finger Domains Protein; E3 Ubiquitin-Protein Ligase CHFR; EC 2.3.2.27; RNF116;

Function

E3 ubiquitin-protein ligase that functions in the antephase checkpoint by actively delaying passage into mitosis in response to microtubule poisons. Acts in early prophase before chromosome condensation, when the centrosome move apart from each other along the periphery of the nucleus. Probably involved in signaling the presence of mitotic stress caused by microtubule poisons by mediating the 'Lys-48'-linked ubiquitination of target proteins, leading to their degradation by the proteasome. Promotes the ubiquitination and subsequent degradation of AURKA and PLK1. Probably acts as a tumor suppressor, possibly by mediating the polyubiquitination of HDAC1, leading to its degradation. May also promote the formation of 'Lys-63'-linked polyubiquitin chains and functions with the specific ubiquitin-conjugating UBC13-MMS2 (UBE2N-UBE2V2) heterodimer. Substrates that are polyubiquitinated at 'Lys-63' are usually not targeted for degradation, but are rather involved in signaling cellular stress.

Biological Process

Cell division Source: UniProtKB-KW
Meiotic spindle checkpoint Source: UniProtKB
Mitotic cell cycle checkpoint Source: UniProtKB
Modification-dependent protein catabolic process Source: UniProtKB
Positive regulation of proteasomal ubiquitin-dependent protein catabolic process Source: Ensembl
Positive regulation of protein ubiquitination Source: Ensembl
Protein destabilization Source: Ensembl
Protein polyubiquitination Source: UniProtKB
Protein ubiquitination Source: GO_Central
Ubiquitin-dependent protein catabolic process Source: GO_Central

Cellular Location

PML body

PTM

Poly-ADP-ribosylated. In addition to binding non covalently poly(ADP-ribose) via its PBZ-type zinc finger, the protein is also covalently poly-ADP-ribosylated by PARP1.
Autoubiquitinated; may regulate its cellular level.
Phosphorylated by PKB. Phosphorylation may affect its E3 ligase activity.

Baretti, M., Karunasena, E., Zahurak, M., Walker, R., Zhao, Y., Pisanic 2nd, T. R., ... & Azad, N. S. (2021). A phase 2 trial of gemcitabine and docetaxel in patients with metastatic colorectal adenocarcinoma with methylated checkpoint with forkhead and ring finger domain promoter and/or microsatellite instability phenotype. Clinical and translational science.

Luo, H., Zhou, Z., Huang, S., Ma, M., Zhao, M., Tang, L., ... & Zhang, P. (2021). CHFR regulates chemoresistance in triple-negative breast cancer through destabilizing ZEB1. Cell death & disease, 12(9), 1-11.

Maekawa, H., Ito, T., Orita, H., Kushida, T., Sakurada, M., Sato, K., ... & Brock, M. V. (2020). Analysis of the methylation of CpG islands in the CDO1, TAC1 and CHFR genes in pancreatic ductal cancer. Oncology letters, 19(3), 2197-2204.

Yang, S., He, F., Dai, M., Pan, J., Wang, J., & Ye, B. (2019). CHFR promotes the migration of human gastric cancer cells by inducing epithelial-to-mesenchymal transition in a HDAC1-dependent manner. OncoTargets and therapy, 12, 1075.

Dai, D., Zhou, B., Xu, W., Jin, H., & Wang, X. (2019). CHFR promoter hypermethylation is associated with gastric cancer and plays a protective role in gastric cancer process. Journal of Cancer, 10(4), 949.

Ding, Y., Lian, H. F., & Du, Y. (2018). Clinicopathological significance of CHFR promoter methylation in gastric cancer: a meta-analysis. Oncotarget, 9(11), 10083.

Zhang, D., Xu, X. L., Li, F., Sun, H. C., Cui, Y. Q., Liu, S., & Xu, P. Y. (2017). Upregulation of the checkpoint protein CHFR is associated with tumor suppression in pancreatic cancers. Oncology letters, 14(6), 8042-8050.

Sun, Z., Liu, J., Jing, H., Dong, S. X., & Wu, J. (2017). The diagnostic and prognostic value of CHFR hypermethylation in colorectal cancer, a meta-analysis and literature review. Oncotarget, 8(51), 89142.

Wang, C., Ma, W., Wei, R., Zhang, X., Shen, N., & Shang, L. (2017). Clinicopathological significance of CHFR methylation in non-small cell lung cancer: a systematic review and meta-analysis. Oncotarget, 8(65), 109732.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
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For research use only. Not intended for any clinical use.

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