Mouse Anti-CHRM2 Recombinant Antibody (CBFYM-0998) (CBMAB-M1149-FY)

Name: Mouse Anti-CHRM2 Recombinant Antibody (CBFYM-0998)
SKU: CBMAB-M1149-FY
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

CBFYM-0998

Application

WB, IP, IHC

Immunogen

i3 loop of m2 receptor fusion protein.

Host Species

Mouse

Specificity

Human, Mouse, Rat

Antibody Isotype

IgG2a

Clonality

Monoclonal Antibody

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Application	Note
WB	1:100-1:1,000
IP	1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)
IHC	1:50-1:500

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS, 0.1% gelatin

Preservative

< 0.1% sodium azide

Concentration

0.2 mg/ml

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

More Infomation

Target

Full Name

Cholinergic Receptor Muscarinic 2

Introduction

The muscarinic cholinergic receptors belong to a larger family of G protein-coupled receptors. The functional diversity of these receptors is defined by the binding of acetylcholine to these receptors and includes cellular responses such as adenylate cyclase inhibition, phosphoinositide degeneration, and potassium channel mediation. Muscarinic receptors influence many effects of acetylcholine in the central and peripheral nervous system. The muscarinic cholinergic receptor 2 is involved in mediation of bradycardia and a decrease in cardiac contractility. Multiple alternatively spliced transcript variants have been described for this gene.

Entrez Gene ID

1129

UniProt ID

P08172

Alternative Names

Cholinergic Receptor Muscarinic 2; Acetylcholine Receptor, Muscarinic 2; Muscarinic Acetylcholine Receptor M2; Cholinergic Receptor, Muscarinic 2; Muscarinic M2 Receptor; 7TM Receptor; HM2

Function

The muscarinic acetylcholine receptor mediates various cellular responses, including inhibition of adenylate cyclase, breakdown of phosphoinositides and modulation of potassium channels through the action of G proteins. Primary transducing effect is adenylate cyclase inhibition. Signaling promotes phospholipase C activity, leading to the release of inositol trisphosphate (IP3); this then triggers calcium ion release into the cytosol.

Biological Process

Adenylate cyclase-inhibiting G protein-coupled acetylcholine receptor signaling pathway Source: GO_Central
Adenylate cyclase-modulating G protein-coupled receptor signaling pathway Source: ProtInc
Chemical synaptic transmission Source: GO_Central
G protein-coupled acetylcholine receptor signaling pathway Source: UniProtKB
G protein-coupled receptor signaling pathway Source: Reactome
G protein-coupled receptor signaling pathway, coupled to cyclic nucleotide second messenger Source: GO_Central
Membrane organization Source: Reactome
Nervous system development Source: ProtInc
Phospholipase C-activating G protein-coupled acetylcholine receptor signaling pathway Source: ProtInc
Regulation of heart contraction Source: ProtInc
Regulation of smooth muscle contraction Source: GO_Central
Response to virus Source: UniProtKB

Cellular Location

Cell membrane; Postsynaptic cell membrane. Phosphorylation in response to agonist binding promotes receptor internalization.

Involvement in disease

Major depressive disorder (MDD): A common psychiatric disorder. It is a complex trait characterized by one or more major depressive episodes without a history of manic, mixed, or hypomanic episodes. A major depressive episode is characterized by at least 2 weeks during which there is a new onset or clear worsening of either depressed mood or loss of interest or pleasure in nearly all activities. Four additional symptoms must also be present including changes in appetite, weight, sleep, and psychomotor activity; decreased energy; feelings of worthlessness or guilt; difficulty thinking, concentrating, or making decisions; or recurrent thoughts of death or suicidal ideation, plans, or attempts. The episode must be accompanied by distress or impairment in social, occupational, or other important areas of functioning.

Topology

Extracellular: 1-22
Helical: 23-45
Cytoplasmic: 46-59
Helical: 60-80
Extracellular: 81-97
Helical: 98-119
Cytoplasmic: 120-139
Helical: 140-162
Extracellular: 163-184
Helical: 185-209
Cytoplasmic: 210-387
Helical: 388-410
Extracellular: 411-418
Helical: 419-442
Cytoplasmic: 443-466

PTM

Phosphorylated in response to agonist treatment.

Boiko, A. S., Ivanova, S. A., Pozhidaev, I. V., Freidin, M. B., Osmanova, D. Z., Fedorenko, O. Y., ... & Loonen, A. J. (2020). Pharmacogenetics of tardive dyskinesia in schizophrenia: The role of CHRM1 and CHRM2 muscarinic receptors. The World Journal of Biological Psychiatry, 21(1), 72-77.

Zink, N., Bensmann, W., Arning, L., Stock, A. K., & Beste, C. (2019). CHRM2 genotype affects inhibitory control mechanisms during cognitive flexibility. Molecular neurobiology, 56(9), 6134-6141.

Zhang, J., Chen, S. R., Chen, H., & Pan, H. L. (2018). RE1-silencing transcription factor controls the acute-to-chronic neuropathic pain transition and Chrm2 receptor gene expression in primary sensory neurons. Journal of Biological Chemistry, 293(49), 19078-19091.

Chee, L. Y., & Cumming, A. (2018). Polymorphisms in the cholinergic receptors muscarinic (CHRM2 and CHRM3) genes and Alzheimer’s disease. Avicenna journal of medical biotechnology, 10(3), 196.

Sakata, K., & Overacre, A. E. (2017). Promoter IV‐BDNF deficiency disturbs cholinergic gene expression of CHRNA 5, CHRM 2, and CHRM 5: effects of drug and environmental treatments. Journal of neurochemistry, 143(1), 49-64.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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