Human CHRM2 ELISA Kit (V2LY-0626-LY2352)

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Tested Data
Datasheet
SDS
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Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Sensitivity
0.0092 ng/mL
Detection Range
0.02-4.5 ng/mL
Sample Type
Serum, Plasma, cell culture supernates
Specificity
Human
Assay Type
Sandwich
Reactivity
Human
Assay Time
1.5 h
Molecule Mass
51.7 kDa
Components
  • Pre-coated ELISA Plate: 12 wells * 8 detachable strips
  • Standard solution: 0.5ml x1
  • Standard diluent: 3ml x1
  • Streptavidin-HRP: 6ml x1
  • Stop solution: 6ml x1
  • Substrate solution A: 6ml x1
  • Substrate solution B: 6ml x1
  • Wash buffer concentrate (25x): 20ml x1
  • Biotinylated antibody: 1ml x1

Formulations & Storage [For reference only, actual COA shall prevail!]

Storage
Store at 2-8°C
More Infomation

Target

Full Name
Cholinergic Receptor Muscarinic 2
Function
The muscarinic acetylcholine receptor mediates various cellular responses, including inhibition of adenylate cyclase, breakdown of phosphoinositides and modulation of potassium channels through the action of G proteins. Primary transducing effect is adenylate cyclase inhibition. Signaling promotes phospholipase C activity, leading to the release of inositol trisphosphate (IP3); this then triggers calcium ion release into the cytosol.
Biological Process
Adenylate cyclase-inhibiting G protein-coupled acetylcholine receptor signaling pathway Source: GO_Central
Adenylate cyclase-modulating G protein-coupled receptor signaling pathway Source: ProtInc
Chemical synaptic transmission Source: GO_Central
G protein-coupled acetylcholine receptor signaling pathway Source: UniProtKB
G protein-coupled receptor signaling pathway Source: Reactome
G protein-coupled receptor signaling pathway, coupled to cyclic nucleotide second messenger Source: GO_Central
Membrane organization Source: Reactome
Nervous system development Source: ProtInc
Phospholipase C-activating G protein-coupled acetylcholine receptor signaling pathway Source: ProtInc
Regulation of heart contraction Source: ProtInc
Regulation of smooth muscle contraction Source: GO_Central
Response to virus Source: UniProtKB
Cellular Location
Cell membrane; Postsynaptic cell membrane. Phosphorylation in response to agonist binding promotes receptor internalization.
Involvement in disease
Major depressive disorder (MDD): A common psychiatric disorder. It is a complex trait characterized by one or more major depressive episodes without a history of manic, mixed, or hypomanic episodes. A major depressive episode is characterized by at least 2 weeks during which there is a new onset or clear worsening of either depressed mood or loss of interest or pleasure in nearly all activities. Four additional symptoms must also be present including changes in appetite, weight, sleep, and psychomotor activity; decreased energy; feelings of worthlessness or guilt; difficulty thinking, concentrating, or making decisions; or recurrent thoughts of death or suicidal ideation, plans, or attempts. The episode must be accompanied by distress or impairment in social, occupational, or other important areas of functioning.
Topology
Extracellular: 1-22
Helical: 23-45
Cytoplasmic: 46-59
Helical: 60-80
Extracellular: 81-97
Helical: 98-119
Cytoplasmic: 120-139
Helical: 140-162
Extracellular: 163-184
Helical: 185-209
Cytoplasmic: 210-387
Helical: 388-410
Extracellular: 411-418
Helical: 419-442
Cytoplasmic: 443-466
PTM
Phosphorylated in response to agonist treatment.

Boiko, A. S., Ivanova, S. A., Pozhidaev, I. V., Freidin, M. B., Osmanova, D. Z., Fedorenko, O. Y., ... & Loonen, A. J. (2020). Pharmacogenetics of tardive dyskinesia in schizophrenia: The role of CHRM1 and CHRM2 muscarinic receptors. The World Journal of Biological Psychiatry, 21(1), 72-77.

Zink, N., Bensmann, W., Arning, L., Stock, A. K., & Beste, C. (2019). CHRM2 genotype affects inhibitory control mechanisms during cognitive flexibility. Molecular neurobiology, 56(9), 6134-6141.

Zhang, J., Chen, S. R., Chen, H., & Pan, H. L. (2018). RE1-silencing transcription factor controls the acute-to-chronic neuropathic pain transition and Chrm2 receptor gene expression in primary sensory neurons. Journal of Biological Chemistry, 293(49), 19078-19091.

Chee, L. Y., & Cumming, A. (2018). Polymorphisms in the cholinergic receptors muscarinic (CHRM2 and CHRM3) genes and Alzheimer’s disease. Avicenna journal of medical biotechnology, 10(3), 196.

Sakata, K., & Overacre, A. E. (2017). Promoter IV‐BDNF deficiency disturbs cholinergic gene expression of CHRNA 5, CHRM 2, and CHRM 5: effects of drug and environmental treatments. Journal of neurochemistry, 143(1), 49-64.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Alternative Versions

Rat Chrm2 ELISA Kit (CAT#: V2LY-0626-LY1705)

Mouse Chrm2 ELISA Kit (CAT#: V2LY-0626-LY2703)

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Mouse Chrm2 ELISA Kit (CAT#: V2LY-0626-LY2703)

Mouse Anti-CHRM2 Monoclonal Antibody (1400) (CAT#: CBMAB-1400-YC)

Mouse Anti-CHRM2 Monoclonal Antibody (4B5) (CAT#: CBMAB-1402-YC)

Rat Chrm2 ELISA Kit (CAT#: V2LY-0626-LY1705)

Rabbit Anti-CHRM2 Recombinant Antibody (EG682) (CAT#: CBMAB-EN823-LY)

Mouse Anti-CHRM2 Recombinant Antibody (CBFYM-0998) (CAT#: CBMAB-M1149-FY)

Isotype control

For research use only. Not intended for any clinical use.

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