Mouse Anti-CHRNA2 Recombinant Antibody (CBWJN-1064) (CBMAB-N0668-WJ)

Name: Mouse Anti-CHRNA2 Recombinant Antibody (CBWJN-1064)
SKU: CBMAB-N0668-WJ
Rating: 5 (1 reviews)

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Datasheet

Summary

Host Animal

Mouse

Specificity

Human, Mouse, Rat

Clone

CBWJN-1064

Antibody Isotype

IgG1, κ

Application

ELISA, IF, WB, IP

Basic Information

Immunogen

Amino acids 364-474 mapping near the C-terminus of AChRα2 of human.

Host Species

Mouse

Specificity

Human, Mouse, Rat

Antibody Isotype

IgG1, κ

Clonality

Monoclonal Antibody

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Application	Note
WB	1:100-1:1,000
IP	1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)
IF(ICC)	1:50-1:500
ELISA	1:100-1:1,000

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS, 0.1% gelatin

Preservative

< 0.1% sodium azide

Concentration

0.2 mg/ml

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name

cholinergic receptor nicotinic alpha 2 subunit

Introduction

Nicotinic acetylcholine receptors (nAChRs) are ligand-gated ion channels formed by a pentameric arrangement of alpha and beta subunits to create distinct muscle and neuronal receptors. Neuronal receptors are found throughout the peripheral and central nervous system where they are involved in fast synaptic transmission. This gene encodes an alpha subunit that is widely expressed in the brain. The proposed structure for nAChR subunits is a conserved N-terminal extracellular domain followed by three conserved transmembrane domains, a variable cytoplasmic loop, a fourth conserved transmembrane domain, and a short C-terminal extracellular region. Mutations in this gene cause autosomal dominant nocturnal frontal lobe epilepsy type 4. Single nucleotide polymorphisms (SNPs) in this gene have been associated with nicotine dependence. [provided by RefSeq, Nov 2009]

Entrez Gene ID

Rat	170945
Chicken	395607

UniProt ID

Rat	P12389
Chicken	P09480

Alternative Names

Cholinergic Receptor Nicotinic Alpha 2 Subunit; Cholinergic Receptor, Nicotinic, Alpha Polypeptide 2 (Neuronal); Acetylcholine Receptor, Nicotinic, Alpha 2 (Neuronal); Cholinergic Receptor, Nicotinic, Alpha 2 (Neuronal); Cholinergic Receptor, Nicotinic Alpha 2;

Function

After binding acetylcholine, the AChR responds by an extensive change in conformation that affects all subunits and leads to opening of an ion-conducting channel across the plasma membrane.

Biological Process

Chemical synaptic transmission Source: GO_Central
Ion transmembrane transport Source: GO_Central
Ion transport Source: UniProtKB
Nervous system process Source: GO_Central
Regulation of membrane potential Source: GO_Central
Response to nicotine Source: GO_Central
Signal transduction Source: UniProtKB
Synaptic transmission, cholinergic Source: GO_Central

Cellular Location

Postsynaptic cell membrane; Cell membrane

Involvement in disease

Epilepsy, nocturnal frontal lobe, 4 (ENFL4): An autosomal dominant focal epilepsy characterized by nocturnal seizures associated with fear sensation, tongue movements, and nocturnal wandering, closely resembling nightmares and sleep walking.
Seizures, benign familial infantile, 6 (BFIS6): A form of benign familial infantile epilepsy, a neurologic disorder characterized by afebrile seizures occurring in clusters during the first year of life, without neurologic sequelae. BFIS6 inheritance is autosomal dominant.

Topology

Extracellular: 27-264
Helical: 265-289
Helical: 297-315
Helical: 331-352
Cytoplasmic: 353-502
Helical: 503-521

More Infomation

References

Ma, Y., Liu, S., Jun, H., & Wu, J. (2021). CHRNA2: a new paradigm in beige thermoregulation and metabolism. Trends in cell biology.

Villa, C., Colombo, G., Meneghini, S., Gotti, C., Moretti, M., Ferini-Strambi, L., ... & Combi, R. (2019). CHRNA2 and nocturnal frontal lobe epilepsy: Identification and characterization of a novel loss of function mutation. Frontiers in molecular neuroscience, 12, 17.

Nichol, H., Amilhon, B., Manseau, F., Badrinarayanan, S., & Williams, S. (2018). Electrophysiological and morphological characterization of Chrna2 cells in the subiculum and CA1 of the Hippocampus: an optogenetic investigation. Frontiers in cellular neuroscience, 12, 32.

Hilscher, M. M., Leão, R. N., Edwards, S. J., Leão, K. E., & Kullander, K. (2017). Chrna2-Martinotti cells synchronize layer 5 type A pyramidal cells via rebound excitation. PLoS biology, 15(2), e2001392.

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Protocols

Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
Other Protocols

Associated Products

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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Documents

Datasheet
SDS
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Second antibody and isotype control

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