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Mouse Anti-CHRNA5 Recombinant Antibody (BA0287) (CBMAB-0243CQ)

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Published Data
CBMAB-0243CQ-1
Mouse Anti-CHRNA5 Recombinant Antibody (BA0287) (CBMAB-0243CQ) in WB
Based on the predicted sizes of the PCR products, mRNAs for the CHRNA3, CHRNA4, CHRNA5, CHRNA6, CHRNA7, CHRNB2, and CHRNB4 were detected in HBO cells. ...View More
Qian, J., Mummalaneni, S., Larsen, J., Grider, J. R., Spielman, A. I., Özdener, M. H., & Lyall, V. (2018). Nicotinic acetylcholine receptor (CHRN) expression and function in cultured human adult fungiform (HBO) taste cells. PLoS One, 13(3), e0194089.
Distributed under Open Access license CC BY 4.0, without modification.
CBMAB-0243CQ-2
Mouse Anti-CHRNA5 Recombinant Antibody (BA0287) (CBMAB-0243CQ) in IF
Immunofluorescence staining of CHRNA4 and CHRNA5 in HBO cells.
Shows immunostaining of CHRNA4 (A; α4) and CHRNA5 (B; α5) in HBO cells using 20x and 40x objectives (total 200x and 400x magnification). The panels show merged confocal images of DAPI (blue) and secondary antibody fluorescence (green). The negative control (NC) without primary antibody is also shown. The horizontal red lines represent 10 μm. ...View More
Qian, J., Mummalaneni, S., Larsen, J., Grider, J. R., Spielman, A. I., Özdener, M. H., & Lyall, V. (2018). Nicotinic acetylcholine receptor (CHRN) expression and function in cultured human adult fungiform (HBO) taste cells. PLoS One, 13(3), e0194089.
Distributed under Open Access license CC BY 4.0, without modification.
CBMAB-0243CQ-3
Mouse Anti-CHRNA5 Recombinant Antibody (BA0287) (CBMAB-0243CQ) in IF
Immunofluorescence staining of nAChR subunits and BDNF in STC-1 cells.
(Panel A) Immunostaining of nAChR α3, α4, and α5. (Panel B) Immunostaining of nAChR α7, β2, and β4. (Panel C) Negative control (NC) without primary antibody and immunostaining of BDNF. Blue color represents staining of cell nuclei with DAPI. The panels show merged confocal images of DAPI and secondary antibody fluorescence. ...View More
Qian, J., Mummalaneni, S. K., Alkahtani, R. M., Mahavadi, S., Murthy, K. S., Grider, J. R., & Lyall, V. (2016). Nicotine-induced effects on nicotinic acetylcholine receptors (nAChRs), Ca2+ and brain-derived neurotrophic factor (BDNF) in STC-1 cells. PLoS One, 11(11), e0166565.
Distributed under Open Access license CC BY 4.0, without modification.
CBMAB-0243CQ-4
Mouse Anti-CHRNA5 Recombinant Antibody (BA0287) (CBMAB-0243CQ) in WB
Co-IP of nAChRs in STC-1 cell lysates.
In STC-1 cell lysates nAChRs α5 and β4 proteins were immunoprecipitated by AChRα3 antibody (A, B), and nAChR α4 protein was immunoprecipitated by AChRβ2 antibody (C). IP = immunoprecipitation; WB = Western blot; IgG = negative control. ...View More
Qian, J., Mummalaneni, S. K., Alkahtani, R. M., Mahavadi, S., Murthy, K. S., Grider, J. R., & Lyall, V. (2016). Nicotine-induced effects on nicotinic acetylcholine receptors (nAChRs), Ca2+ and brain-derived neurotrophic factor (BDNF) in STC-1 cells. PLoS One, 11(11), e0166565.
Distributed under Open Access license CC BY 4.0, without modification.
Datasheet
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Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal
Mouse
Clone
BA0287
Application
ELISA, IF, WB, IP, IHC-P
Immunogen
Amino acids 370-419 mapping within a cytoplasmic domain of AChRα5 of human.
Host Species
Mouse
Specificity
Human
Antibody Isotype
IgG2a, κ
Clonality
Monoclonal Antibody
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.
ApplicationNote
WB1:100-1:1,000
IP1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)
IF(ICC)1:50-1:500
ELISA1:100-1:1,000
IHC-P1:50-1:500

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
PBS, 0.1% gelatin
Preservative
< 0.1% sodium azide
Concentration
0.2 mg/ml
Purity
>95% as determined by analysis by SDS-PAGE
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
More Infomation

Target

Full Name
Cholinergic Receptor Nicotinic Alpha 5 Subunit
Introduction
Nicotinic acetylcholine receptors (nAChRs), such as CHRNA5, are members of a superfamily of ligand-gated ion channels that mediate fast signal transmission at synapses. The predicted 468-amino acid protein has a putative 22-amino acid signal peptide. The mature protein is 87% identical to rat alpha-5 protein. On Northern blots, alpha-5 was expressed in almost all neuronal and nonneuronal cells tested as 2 major transcripts of 2.7 and 2.1 kb, with additional mRNAs seen in 1 neuroblastoma cell line. The nAChRs are thought to be (hetero)pentamers composed of homologous subunits.
Entrez Gene ID
Human1138
Mouse110835
Rat25102
UniProt ID
HumanP30532
MouseQ2MKA5
RatP20420
Alternative Names
Cholinergic Receptor, Nicotinic, Alpha 5 (Neuronal); Cholinergic Receptor, Neuronal Nicotinic, Alpha Polypeptide-5; Neuronal Nicotinic Acetylcholine Receptor, Alpha5 Subunit; Acetylcholine Receptor, Nicotinic, Alpha 5 (Neuronal); Cholinergic Receptor, Nicotinic, Alpha Polypeptide 5; Acetylcholine Receptor; Alpha 5 (Neuronal); Nicotinic; NACHRA5; LNCR2
Function
After binding acetylcholine, the AChR responds by an extensive change in conformation that affects all subunits and leads to opening of an ion-conducting channel across the plasma membrane.
Biological Process
Behavioral response to nicotine Source: UniProtKB
Chemical synaptic transmission Source: GO_Central
Ion transmembrane transport Source: GO_Central
Nervous system process Source: GO_Central
Regulation of membrane potential Source: GO_Central
Regulation of synaptic vesicle exocytosis Source: Ensembl
Response to nicotine Source: GO_Central
Signal transduction Source: GO_Central
Synaptic transmission, cholinergic Source: GO_Central
Cellular Location
Postsynaptic cell membrane; Cell membrane
Topology
Extracellular: 23-254
Helical: 255-275
Helical: 282-302
Helical: 317-337
Cytoplasmic: 338-429
Helical: 430-451
Extracellular: 452-468

Forget, B., Icick, R., Robert, J., Correia, C., Prevost, M. S., Gielen, M., ... & Maskos, U. (2021). Alterations in nicotinic receptor alpha5 subunit gene differentially impact early and later stages of cocaine addiction: a translational study in transgenic rats and patients. Progress in Neurobiology, 197, 101898.

Brynildsen, J. K., & Blendy, J. A. (2021). Linking the CHRNA5 SNP to drug abuse liability: From circuitry to cellular mechanisms. Neuropharmacology, 186, 108480.

Chen, L. S., Baker, T. B., Miller, J. P., Bray, M., Smock, N., Chen, J., ... & Bierut, L. J. (2020). Genetic Variant in CHRNA5 and Response to Varenicline and Combination Nicotine Replacement in a randomized placebo‐controlled trial. Clinical Pharmacology & Therapeutics, 108(6), 1315-1325.

Venkatesan, S., & Lambe, E. K. (2020). Chrna5 is essential for a rapid and protected response to optogenetic release of endogenous acetylcholine in prefrontal cortex. Journal of Neuroscience, 40(38), 7255-7268.

Tam, A., Obeidat, M., Hackett, T. L., Dorscheid, D. R., Singhera, G., Hogg, J. C., & Sin, D. D. (2019). Cholinergic Receptor Nicotinic Alpha 5 Expression in the Airways of Patients with Chronic Obstructive Pulmonary Disease. In B64. COPD: MECHANISM AND TREATMENT (pp. A3845-A3845). American Thoracic Society.

Lin, C. H., Lee, H. H., Kuei, C. H., Lin, H. Y., Lu, L. S., Lee, F. P., ... & Lin, Y. F. (2019). Nicotinic acetylcholine receptor subunit Alpha-5 promotes radioresistance via recruiting E2F activity in oral squamous cell carcinoma. Journal of clinical medicine, 8(9), 1454.

Tomaz, P. R. X., Santos, J. R., Scholz, J., Abe, T. O., Gaya, P. V., Negrão, A. B., ... & Santos, P. C. J. L. (2018). Cholinergic receptor nicotinic alpha 5 subunit polymorphisms are associated with smoking cessation success in women. BMC medical genetics, 19(1), 1-8.

Cingir Koker, S., Jahja, E., Shehwana, H., Keskus, A. G., & Konu, O. (2018). Cholinergic Receptor Nicotinic Alpha 5 (CHRNA5) RNAi is associated with cell cycle inhibition, apoptosis, DNA damage response and drug sensitivity in breast cancer. PloS one, 13(12), e0208982.

Köker, Ş. C. (2018). Effects of Cholinergic Receptor Nicotinic Alpha 5 (CHRNA5) RNAi on apoptosis, DNA damage response, drug sensitivity, and HSA-MIR-495-3P overexpression in breast cancer (Doctoral dissertation, Bilkent University).

Morton, G., Nasirova, N., Sparks, D. W., Brodsky, M., Sivakumaran, S., Lambe, E. K., & Turner, E. E. (2018). Chrna5-expressing neurons in the interpeduncular nucleus mediate aversion primed by prior stimulation or nicotine exposure. Journal of Neuroscience, 38(31), 6900-6920.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

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For research use only. Not intended for any clinical use.

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