Mouse Anti-CHRNA7 Recombinant Antibody (CBT3118) (V2LY-0625-LY748)

Name: Mouse Anti-CHRNA7 Recombinant Antibody (CBT3118)
SKU: V2LY-0625-LY748
Rating: 5 (1 reviews)

See all CHRNA7 antibodies

Compare Online Inquiry

Tested Data

Mouse Anti-CHRNA7 Recombinant Antibody (CBT3118) (V2LY-0625-LY748) in ELISA

Mouse Anti-CHRNA7 Recombinant Antibody (CBT3118) (V2LY-0625-LY748) in WB

Mouse Anti-CHRNA7 Recombinant Antibody (CBT3118) (V2LY-0625-LY748) in FC

Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

CBT3118

Application

WB, FC

Immunogen

Purified recombinant fragment of human CHRNA7 (AA: extra 52-259) expressed in E. Coli.

Host Species

Mouse

Specificity

Human, Rat

Antibody Isotype

IgG1

Clonality

Monoclonal Antibody

Application Notes

Application	Note
WB	1:500-1:2,000
FC	1:200-1:400
ELISA	1:10,000

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS

Preservative

Sodium azide

Concentration

Batch dependent

Purity

> 95% Purity determined by SDS-PAGE.

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

More Infomation

Target

Full Name

Cholinergic Receptor Nicotinic Alpha 7 Subunit

Entrez Gene ID

1139

UniProt ID

P36544

Function

After binding acetylcholine, the AChR responds by an extensive change in conformation that affects all subunits and leads to opening of an ion-conducting channel across the plasma membrane. The channel is blocked by alpha-bungarotoxin.

Biological Process

Acetylcholine receptor signaling pathway Source: ARUK-UCL
Activation of MAPK activity Source: UniProtKB
Calcium ion transport Source: UniProtKB
Cellular calcium ion homeostasis Source: UniProtKB
Chemical synaptic transmission Source: GO_Central
Cognition Source: UniProtKB
Dendrite arborization Source: ARUK-UCL
Dendritic spine organization Source: ARUK-UCL
Ion transmembrane transport Source: ParkinsonsUK-UCL
Ion transport Source: UniProtKB
Learning or memory Source: ARUK-UCL
Memory Source: ARUK-UCL
Modulation of excitatory postsynaptic potential Source: ARUK-UCL
Negative regulation of amyloid-beta formation Source: ARUK-UCL
Negative regulation of tumor necrosis factor production Source: MGI
Nervous system process Source: GO_Central
Positive regulation of amyloid-beta formation Source: ARUK-UCL
Positive regulation of angiogenesis Source: UniProtKB
Positive regulation of cell population proliferation Source: UniProtKB
Positive regulation of CoA-transferase activity Source: ARUK-UCL
Positive regulation of ERK1 and ERK2 cascade Source: ARUK-UCL
Positive regulation of excitatory postsynaptic potential Source: ARUK-UCL
Positive regulation of long-term synaptic potentiation Source: ARUK-UCL
Positive regulation of protein metabolic process Source: ARUK-UCL
Positive regulation of protein phosphorylation Source: ARUK-UCL
Regulation of amyloid fibril formation Source: ARUK-UCL
Regulation of amyloid precursor protein catabolic process Source: ARUK-UCL
Regulation of membrane potential Source: GO_Central
Regulation of neuron death Source: ARUK-UCL
Response to acetylcholine Source: ARUK-UCL
Response to amyloid-beta Source: ARUK-UCL
Response to hypoxia Source: UniProtKB
Response to nicotine Source: UniProtKB
Sensory processing Source: ARUK-UCL
Short-term memory Source: ARUK-UCL
Signal transduction Source: UniProtKB
Synapse organization Source: ARUK-UCL
Synaptic transmission, cholinergic Source: GO_Central

Cellular Location

Postsynaptic cell membrane; Cell membrane. TMEM35A/NACHO promotes its trafficking to the cell membrane (PubMed:27789755). RIC3 promotes its trafficking to the cell membrane (By similarity).

Topology

Extracellular: 23-230
Helical: 231-255
Helical: 262-280
Helical: 296-317
Cytoplasmic: 318-469
Helical: 470-490

PTM

Glycosylations at Asn-46, Asn-90 and Asn-133 are essential for TMEM35A/NACHO-mediated proper subunit assembly and trafficking to the cell membrane.

Wang, L., Liang, D., Xiong, X., Lin, Y., Zhu, J., Yao, Z., ... & Zhang, H. (2021). Repurposing dextromethorphan and metformin for treating nicotine-induced cancer by directly targeting CHRNA7 to inhibit JAK2/STAT3/SOX2 signaling. Oncogene, 40(11), 1974-1987.

Briggs, F. B. (2021). Nicotinic acetylcholine receptors α7 and α9 modifies tobacco smoke risk for multiple sclerosis. Multiple Sclerosis Journal, 27(8), 1166-1174.

Özaltun, M. F., Geyik, S., & Yılmaz, Ş. G. (2021). Screening for Copy Number Variations of the 15q13. 3 Hotspot in CHRNA7 Gene and Expression in Patients with Migraines. Current issues in molecular biology, 43(2), 1090-1113.

Santos, J. R., Tomaz, P. R. X., Scholz, J. R., Gaya, P. V., Abe, T. O., Krieger, J. E., ... & Santos, P. C. J. D. L. (2020). Profile of the Nicotinic Cholinergic Receptor Alpha 7 Subunit Gene Expression is Associated with Response to Varenicline Treatment. Genes, 11(7), 746.

Chan, T., Williams, E., Cohen, O., Eliceiri, B. P., Baird, A., & Costantini, T. W. (2019). CHRFAM7A alters binding to the neuronal alpha-7 nicotinic acetylcholine receptor. Neuroscience letters, 690, 126-131.

Calabrò, M., Mandelli, L., Crisafulli, C., Lee, S. J., Jun, T. Y., Wang, S. M., ... & Serretti, A. (2019). Genes involved in neurodevelopment, neuroplasticity and major depression: no association for CACNA1C, CHRNA7 and MAPK1. Clinical Psychopharmacology and Neuroscience, 17(3), 364.

De Jaco, A., Bernardini, L., Rosati, J., & Maria Tata, A. (2017). Alpha-7 nicotinic receptors in nervous system disorders: from function to therapeutic perspectives. Central Nervous System Agents in Medicinal Chemistry (Formerly Current Medicinal Chemistry-Central Nervous System Agents), 17(2), 100-108.

Yadav, R., Deepshikha, D., & Srivastava, P. (2017). Homology modeling and protein interaction map of CHRNA7 neurogenesis protein. Annals of neurosciences, 24(3), 173-179.

Ask a question We look forward to hearing from you.

0 reviews or Q&As

Purchasing FAQs
Technical FAQs

Have you used Mouse Anti-CHRNA7 Recombinant Antibody (CBT3118)?
Submit a review and get a Coupon or an Amazon gift card. 20% off Coupon

$30 eGift Card
Submit a review

Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Related Products

Mouse Anti-CHRNA7 Recombinant Antibody (CBLNC-159) (CAT#: CBMAB-1373-CN)

Mouse Anti-CHRNA7 Recombinant Antibody (CBWJN-1067) (CAT#: CBMAB-N0671-WJ)

Rat Anti-CHRNA7 Recombinant Antibody (CBWJN-1068) (CAT#: CBMAB-N0672-WJ)

Mouse Anti-CHRNA7 Recombinant Antibody (CBT4265) (CAT#: V2LY-0625-LY747)

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

Online Inquiry

Request bulk or custom quote Second antibody and isotype control

Contact us

Tel: (USA)
(UK)
Fax:

Global Locations

Email:

Online Inquiry