Mouse Anti-CHRNB4 Recombinant Antibody (CBXC-1771) (CBMAB-C4855-CQ)

Name: Mouse Anti-CHRNB4 Recombinant Antibody (CBXC-1771)
SKU: CBMAB-C4855-CQ
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

CBXC-1771

Application

WB, IP, IF, ELISA

Immunogen

Amino acids 336-432 mapping near the C-terminus within a cytoplasmic domain of CHRNB4 of human.

Host Species

Mouse

Specificity

Human

Antibody Isotype

IgG1, κ

Clonality

Monoclonal Antibody

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Application	Note
WB	1:100-1:1,000
IP	1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)
IF(ICC)	1:50-1:500
ELISA	1:10,000

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS, 0.1% gelatin

Preservative

< 0.1% sodium azide

Concentration

0.2 mg/ml

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

More Infomation

Target

Full Name

Cholinergic Receptor Nicotinic Beta 4 Subunit

Introduction

This gene is found within a conserved gene cluster and encodes one of the beta subunits of the nicotinic acetylcholine receptor (nAChRs) superfamily which form ligand-gated ion channels with a central pore that forms a cation channel. Neuronal nAChRs are pentameric structures that can be either homomeric or heteromeric, with heteromeric structures containing both alpha and beta subunits. Each subunit contains an extracellular amino terminus and four transmembrane domains. Nicotine is one of the agonists that binds to the receptor. Variants in this gene have been associated with nicotine dependence and lung cancer. Alternative splicing results in multiple transcript variants encoding different isoforms.

Entrez Gene ID

1143

UniProt ID

P30926

Alternative Names

Cholinergic Receptor Nicotinic Beta 4 Subunit; Acetylcholine Receptor, Nicotinic, Beta 4 (Neuronal); Cholinergic Receptor, Nicotinic, Beta Polypeptide 4; Cholinergic Receptor, Nicotinic, Beta 4 (Neuronal); Cholinergic Receptor, Nicotinic Beta 4;

Function

After binding acetylcholine, the AChR responds by an extensive change in conformation that affects all subunits and leads to opening of an ion-conducting channel across the plasma membrane.

Biological Process

Chemical synaptic transmission Source: GO_Central
Ion transmembrane transport Source: GO_Central
Ion transport Source: UniProtKB
Nervous system process Source: GO_Central
Neutrophil degranulation Source: Reactome
Regulation of membrane potential Source: GO_Central
Regulation of neurotransmitter secretion Source: UniProtKB
Signal transduction Source: UniProtKB
Synaptic transmission, cholinergic Source: ProtInc
Synaptic transmission involved in micturition Source: UniProtKB

Cellular Location

Postsynaptic cell membrane; Cell membrane

Topology

Extracellular: 22-236
Helical: 237-257
Cytoplasmic: 258-265
Helical: 266-286
Extracellular: 287-298
Helical: 299-319
Cytoplasmic: 320-460
Helical: 461-481
Extracellular: 482-498

Chuang, Y. H., Lee, C. H., Lin, C. Y., Liu, C. L., Huang, S. H., Lee, J. Y., ... & Yang, J. M. (2020). An integrated genomic strategy to identify chrnb4 as a diagnostic/prognostic biomarker for targeted therapy in head and neck cancer. Cancers, 12(5), 1324.

Li, N., Liu, K., Dong, S., Ou, L., Li, J., Lai, M., ... & Wang, S. (2020). Identification of CHRNB4 as a Diagnostic/Prognostic Indicator and Therapeutic Target in Human Esophageal Squamous Cell Carcinoma. Frontiers in Oncology, 10, 2523.

Barrie, E. S., Hartmann, K., Lee, S. H., Frater, J. T., Seweryn, M., Wang, D., & Sadee, W. (2017). The CHRNA5/CHRNA3/CHRNB4 nicotinic receptor regulome: genomic architecture, regulatory variants, and clinical associations. Human mutation, 38(1), 112-119.

Kamens, H. M., Silva, C., McCarthy, R., Cox, R. J., & Ehringer, M. A. (2017). No evidence of a role of the β4 subunit of the nicotinic acetylcholine receptor in alcohol-related behaviors. BMC research notes, 10(1), 1-10.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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