Mouse Anti-CIDEA Recombinant Antibody (4B9) (CBMAB-C6695-LY)

Name: Mouse Anti-CIDEA Recombinant Antibody (4B9)
SKU: CBMAB-C6695-LY
Rating: 5 (1 reviews)

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Published Data

Mouse Anti-CIDEA Recombinant Antibody (4B9) (CBMAB-C6695-LY) in WB

The protein levels in brown adipocytes after the thermogenesis test. ...View More

Duan, Y. N., Ge, X., Jiang, H. W., Zhang, H. J., Zhao, Y., Li, J. L., ... & Li, J. Y. (2020). Diphyllin improves high-fat diet-induced obesity in mice through brown and beige adipocytes. Frontiers in endocrinology, 11, 592818.

Distributed under Open Access license CC BY 4.0, without modification.

Mouse Anti-CIDEA Recombinant Antibody (4B9) (CBMAB-C6695-LY) in WB

The protein levels in brown adipocytes after the thermogenesis test. ...View More

Distributed under Open Access license CC BY 4.0, without modification.

Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

4B9

Application

ELISA, IP, WB

Immunogen

Amino acids 1-253 of CIDE-A of human.

Host Species

Mouse

Specificity

Human

Antibody Isotype

IgG2a, κ

Clonality

Monoclonal Antibody

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Application	Note
WB	1:100-1:1,000
IP	1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)
ELISA	1:100-1:1,000

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS, 0.1% gelatin

Preservative

< 0.1% sodium azide

Concentration

0.1 mg/ml

Purity

> 95% Purity determined by SDS-PAGE.

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

More Infomation

Target

Full Name

Cell Death-Inducing DFFA-Like Effector A

Introduction

This gene encodes the homolog of the mouse protein Cidea that has been shown to activate apoptosis. This activation of apoptosis is inhibited by the DNA fragmentation factor DFF45 but not by caspase inhibitors. Mice that lack functional Cidea have higher metabolic rates, higher lipolysis in brown adipose tissue and higher core body temperatures when subjected to cold. These mice are also resistant to diet-induced obesity and diabetes. This suggests that in mice this gene product plays a role in thermogenesis and lipolysis. Alternatively spliced transcripts have been identified. [provided by RefSeq, Aug 2010]

Entrez Gene ID

1149

UniProt ID

O60543

Alternative Names

Cell Death-Inducing DFFA-Like Effector A; Cell Death Activator CIDE-A; CIDE-A;

Function

Acts as a CEBPB coactivator in mammary epithelial cells to control the expression of a subset of CEBPB downstream target genes, including ID2, IGF1, PRLR, SOCS1, SOCS3, XDH, but not casein. By interacting with CEBPB, strengthens the association of CEBPB with the XDH promoter, increases histone acetylation and dissociates HDAC1 from the promoter (By similarity).

Binds to lipid droplets and regulates their enlargement, thereby restricting lipolysis and favoring storage. At focal contact sites between lipid droplets, promotes directional net neutral lipid transfer from the smaller to larger lipid droplets. The transfer direction may be driven by the internal pressure difference between the contacting lipid droplet pair and occurs at a lower rate than that promoted by CIDEC. When overexpressed, induces apoptosis. The physiological significance of its role in apoptosis is unclear.

Biological Process

Apoptotic process Source: UniProtKB-KW
Cell death Source: BHF-UCL
Cellular response to cold Source: Ensembl
Lipid droplet organization Source: Reactome
Lipid metabolic process Source: BHF-UCL
Lipid storage Source: BHF-UCL
Negative regulation of cold-induced thermogenesis Source: YuBioLab
Negative regulation of cytokine production Source: BHF-UCL
Negative regulation of execution phase of apoptosis Source: BHF-UCL
Negative regulation of lipid catabolic process Source: BHF-UCL
Negative regulation of transforming growth factor beta receptor signaling pathway Source: BHF-UCL
Negative regulation of tumor necrosis factor production Source: BHF-UCL
Positive regulation of sequestering of triglyceride Source: BHF-UCL
Regulation of apoptotic DNA fragmentation Source: BHF-UCL
Regulation of apoptotic process Source: GO_Central
Response to stilbenoid Source: Ensembl
Temperature homeostasis Source: BHF-UCL

Cellular Location

Nucleus; Lipid droplet. Enriched at lipid droplet contact sites. Has been shown to localize to mitochondria, where it could interact with UCP1 and hence inhibit UCP1 uncoupling activity (By similarity). These data could not be confirmed (PubMed:18509062).

Involvement in disease

In omental and subcutaneous adipose tissue of obese patients matched for BMI, expression levels correlate with insulin sensitivity. Expression is increased 5-6 fold in the group of patients with high insulin sensitivity, compared to the insulin-resistant group. This observation is consistent with the idea that triglyceride storage in adipocytes plays an important role in sequestering triglycerides and fatty acids away from the circulation and peripheral tissues, thus enhancing insulin sensitivity in liver and muscle.

Sun, X., Wang, Y., Loor, J. J., Bucktrout, R., Shu, X., Jia, H., ... & Li, X. (2019). High expression of cell death-inducing DFFA-like effector a (CIDEA) promotes milk fat content in dairy cows with clinical ketosis. Journal of dairy science, 102(2), 1682-1692.

Jash, S., Banerjee, S., Lee, M. J., Farmer, S. R., & Puri, V. (2019). CIDEA transcriptionally regulates UCP1 for britening and thermogenesis in human fat cells. Iscience, 20, 73-89.

Yasumoto, J., Kasai, H., Yoshimura, K., Otoguro, T., Watashi, K., Wakita, T., ... & Moriishi, K. (2017). Hepatitis B virus prevents excessive viral production via reduction of cell death-inducing DFF45-like effectors. Journal of General Virology, 98(7), 1762-1773.

Nishimoto, Y., Nakajima, S., Tateya, S., Saito, M., Ogawa, W., & Tamori, Y. (2017). Cell death-inducing DNA fragmentation factor A-like effector A and fat-specific protein 27β coordinately control lipid droplet size in brown adipocytes. Journal of Biological Chemistry, 292(26), 10824-10834.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
Other Protocols

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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