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Mouse Anti-CILP Recombinant Antibody (2C5) (CBMAB-C4796-LY)

This product is antibody recognizes CILP. The antibody 2C5 immunoassay techniques such as: ELISA.
See all CILP antibodies

Summary

Host Animal
Mouse
Specificity
Human
Clone
2C5
Antibody Isotype
IgG1, κ
Application
ELISA

Basic Information

Specificity
Human
Antibody Isotype
IgG1, κ
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Purity
> 95% Purity determined by SDS-PAGE.
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Target

Full Name
cartilage intermediate layer protein, nucleotide pyrophosphohydrolase
Introduction
Major alterations in the composition of the cartilage extracellular matrix occur in joint disease, such as osteoarthrosis. This gene encodes the cartilage intermediate layer protein (CILP), which increases in early osteoarthrosis cartilage. The encoded protein was thought to encode a protein precursor for two different proteins; an N-terminal CILP and a C-terminal homolog of NTPPHase, however, later studies identified no nucleotide pyrophosphatase phosphodiesterase (NPP) activity. The full-length and the N-terminal domain of this protein was shown to function as an IGF-1 antagonist. An allelic variant of this gene has been associated with lumbar disc disease. [provided by RefSeq, Sep 2010]
Entrez Gene ID
8483
UniProt ID
O75339
Alternative Names
Cartilage Intermediate Layer Protein; Cartilage Intermediate Layer Protein, Nucleotide Pyrophosphohydrolase; CILP-1; Cartilage Intermediate Layer Protein 1 C1; Cartilage Intermediate Layer Protein 1 C2; Cartilage Intermediate Layer Protein 1; Cartilage Intermediate-Layer Protein; HsT18872;
Function
Probably plays a role in cartilage scaffolding. May act by antagonizing TGF-beta1 (TGFB1) and IGF1 functions. Has the ability to suppress IGF1-induced proliferation and sulfated proteoglycan synthesis, and inhibits ligand-induced IGF1R autophosphorylation. May inhibit TGFB1-mediated induction of cartilage matrix genes via its interaction with TGFB1. Overexpression may lead to impair chondrocyte growth and matrix repair and indirectly promote inorganic pyrophosphate (PPi) supersaturation in aging and osteoarthritis cartilage.
Biological Process
Cellular response to transforming growth factor beta stimulus Source: BHF-UCL
Dephosphorylation Source: GOC
Negative regulation of gene expression Source: BHF-UCL
Negative regulation of insulin-like growth factor receptor signaling pathway Source: BHF-UCL
Negative regulation of SMAD protein signal transduction Source: BHF-UCL
Cellular Location
Extracellular matrix
Involvement in disease
Intervertebral disc disease (IDD):
A common musculo-skeletal disorder caused by degeneration of intervertebral disks of the lumbar spine. It results in low-back pain and unilateral leg pain.
PTM
Cleaved into 2 chains possibly by a furin-like protease upon or preceding secretion.

Liu, L., He, J., Liu, C., Yang, M., Fu, J., Yi, J., ... & Feng, C. (2021). Cartilage intermediate layer protein affects the progression of intervertebral disc degeneration by regulating the extracellular microenvironment. International Journal of Molecular Medicine, 47(2), 475-484.

Wang, C., Jian, W., Luo, Q., Cui, J., Qing, Y., Qin, C., ... & Chen, W. (2021). Prognostic value of cartilage intermediate layer protein 1 in chronic heart failure. ESC Heart Failure.

Mamoor, S. (2020). The cartilage intermediate protein, CILP, is differentially expressed in brain metastatic breast cancer.

van Nieuwenhoven, F. A., Munts, C., op’t Veld, R. C., González, A., Díez, J., Heymans, S., ... & van Bilsen, M. (2018, December). Cartilage intermediate layer protein (CILP): A novel mediator of cardiac extracellular matrix remodelling. In INTERNATIONAL JOURNAL OF EXPERIMENTAL PATHOLOGY (Vol. 99, No. 6, pp. A10-A11). 111 RIVER ST, HOBOKEN 07030-5774, NJ USA: WILEY.

Zhang, C. L., Zhao, Q., Liang, H., Qiao, X., Wang, J. Y., Wu, D., ... & Li, L. (2018). Cartilage intermediate layer protein-1 alleviates pressure overload-induced cardiac fibrosis via interfering TGF-β1 signaling. Journal of molecular and cellular cardiology, 116, 135-144.

He, J., Feng, C., Sun, J., Lu, K., Chu, T., Zhou, Y., & Pan, Y. (2018). Cartilage intermediate layer protein is regulated by mechanical stress and affects extracellular matrix synthesis. Molecular medicine reports, 17(4), 6130-6137.

van Nieuwenhoven, F. A., Munts, C., op’t Veld, R. C., González, A., Díez, J., Heymans, S., ... & van Bilsen, M. (2017). Cartilage intermediate layer protein 1 (CILP1): a novel mediator of cardiac extracellular matrix remodelling. Scientific reports, 7(1), 1-9.

Shindo, K., Asakura, M., Min, K. D., Ito, S., Fu, H. Y., Yamazaki, S., ... & Kitakaze, M. (2017). Cartilage intermediate layer protein 1 suppresses TGF-β signaling in cardiac fibroblasts. International Journal of Gerontology, 11(2), 67-74.

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For research use only. Not intended for any clinical use.

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