Human CILP ELISA Kit (V2LY-0626-LY5560)

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Tested Data
Datasheet
SDS
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Basic Information

Sensitivity
0.026 ng/mL
Detection Range
0.05-10 ng/mL
Sample Type
Serum, Plasma, cell culture supernates
Specificity
Human
Assay Type
Sandwich
Reactivity
Human
Assay Time
1.5 h
Molecule Mass
132.6 kDa
Components
  • Pre-coated ELISA Plate: 12 wells * 8 detachable strips
  • Standard solution: 0.5ml x1
  • Standard diluent: 3ml x1
  • Streptavidin-HRP: 6ml x1
  • Stop solution: 6ml x1
  • Substrate solution A: 6ml x1
  • Substrate solution B: 6ml x1
  • Wash buffer concentrate (25x): 20ml x1
  • Biotinylated antibody: 1ml x1

Formulations & Storage [For reference only, actual COA shall prevail!]

Storage
Store at 2-8°C
More Infomation

Target

Full Name
cartilage intermediate layer protein, nucleotide pyrophosphohydrolase
Function
Probably plays a role in cartilage scaffolding. May act by antagonizing TGF-beta1 (TGFB1) and IGF1 functions. Has the ability to suppress IGF1-induced proliferation and sulfated proteoglycan synthesis, and inhibits ligand-induced IGF1R autophosphorylation. May inhibit TGFB1-mediated induction of cartilage matrix genes via its interaction with TGFB1. Overexpression may lead to impair chondrocyte growth and matrix repair and indirectly promote inorganic pyrophosphate (PPi) supersaturation in aging and osteoarthritis cartilage.
Biological Process
Cellular response to transforming growth factor beta stimulus Source: BHF-UCL
Dephosphorylation Source: GOC
Negative regulation of gene expression Source: BHF-UCL
Negative regulation of insulin-like growth factor receptor signaling pathway Source: BHF-UCL
Negative regulation of SMAD protein signal transduction Source: BHF-UCL
Cellular Location
Extracellular matrix
Involvement in disease
Intervertebral disc disease (IDD):
A common musculo-skeletal disorder caused by degeneration of intervertebral disks of the lumbar spine. It results in low-back pain and unilateral leg pain.
PTM
Cleaved into 2 chains possibly by a furin-like protease upon or preceding secretion.

Liu, L., He, J., Liu, C., Yang, M., Fu, J., Yi, J., ... & Feng, C. (2021). Cartilage intermediate layer protein affects the progression of intervertebral disc degeneration by regulating the extracellular microenvironment. International Journal of Molecular Medicine, 47(2), 475-484.

Wang, C., Jian, W., Luo, Q., Cui, J., Qing, Y., Qin, C., ... & Chen, W. (2021). Prognostic value of cartilage intermediate layer protein 1 in chronic heart failure. ESC Heart Failure.

Mamoor, S. (2020). The cartilage intermediate protein, CILP, is differentially expressed in brain metastatic breast cancer.

van Nieuwenhoven, F. A., Munts, C., op’t Veld, R. C., González, A., Díez, J., Heymans, S., ... & van Bilsen, M. (2018, December). Cartilage intermediate layer protein (CILP): A novel mediator of cardiac extracellular matrix remodelling. In INTERNATIONAL JOURNAL OF EXPERIMENTAL PATHOLOGY (Vol. 99, No. 6, pp. A10-A11). 111 RIVER ST, HOBOKEN 07030-5774, NJ USA: WILEY.

Zhang, C. L., Zhao, Q., Liang, H., Qiao, X., Wang, J. Y., Wu, D., ... & Li, L. (2018). Cartilage intermediate layer protein-1 alleviates pressure overload-induced cardiac fibrosis via interfering TGF-β1 signaling. Journal of molecular and cellular cardiology, 116, 135-144.

He, J., Feng, C., Sun, J., Lu, K., Chu, T., Zhou, Y., & Pan, Y. (2018). Cartilage intermediate layer protein is regulated by mechanical stress and affects extracellular matrix synthesis. Molecular medicine reports, 17(4), 6130-6137.

van Nieuwenhoven, F. A., Munts, C., op’t Veld, R. C., González, A., Díez, J., Heymans, S., ... & van Bilsen, M. (2017). Cartilage intermediate layer protein 1 (CILP1): a novel mediator of cardiac extracellular matrix remodelling. Scientific reports, 7(1), 1-9.

Shindo, K., Asakura, M., Min, K. D., Ito, S., Fu, H. Y., Yamazaki, S., ... & Kitakaze, M. (2017). Cartilage intermediate layer protein 1 suppresses TGF-β signaling in cardiac fibroblasts. International Journal of Gerontology, 11(2), 67-74.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Related Products

Mouse Anti-CILP Recombinant Antibody (CBWJC-2717) (CAT#: CBMAB-C3812WJ)

Mouse Anti-CILP Recombinant Antibody (2C5) (CAT#: CBMAB-C4796-LY)

Isotype control

For research use only. Not intended for any clinical use.

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