Mouse Anti-CISD2 Recombinant Antibody (569) (CBMAB-AP1511LY)

Name: Mouse Anti-CISD2 Recombinant Antibody (569)
SKU: CBMAB-AP1511LY
Rating: 5 (1 reviews)

See all CISD2 antibodies

Compare Online Inquiry

Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

569

Application

ELISA, IHC, WB

Immunogen

CDGSH iron sulfur domain 2.

Host Species

Mouse

Specificity

Human, Mouse, Rat

Antibody Isotype

IgG2a

Clonality

Monoclonal Antibody

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS, 50% glycerol

Preservative

0.02% sodium azide

Concentration

Batch dependent

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

More Infomation

Target

Full Name

CDGSH Iron Sulfur Domain 2

Introduction

The protein encoded by this gene is a zinc finger protein that localizes to the endoplasmic reticulum. The encoded protein binds an iron/sulfur cluster and may be involved in calcium homeostasis. Defects in this gene are a cause of Wolfram syndrome 2. [provided by RefSeq, Mar 2011]

Entrez Gene ID

Human	493856
Mouse	67006
Rat	295457

UniProt ID

Human	Q8N5K1
Mouse	Q9CQB5
Rat	D4AAE9

Alternative Names

CDGSH Iron Sulfur Domain 2; Endoplasmic Reticulum Intermembrane Small Protein; Nutrient-Deprivation Autophagy Factor-1; Zinc Finger, CDGSH-Type Domain 2; MitoNEET-Related 1 Protein; Miner1; NAF-1; ZCD2;

Function

Regulator of autophagy that contributes to antagonize BECN1-mediated cellular autophagy at the endoplasmic reticulum. Participates in the interaction of BCL2 with BECN1 and is required for BCL2-mediated depression of endoplasmic reticulum Ca2+ stores during autophagy. Contributes to BIK-initiated autophagy, while it is not involved in BIK-dependent activation of caspases. Involved in life span control, probably via its function as regulator of autophagy.

Biological Process

Autophagy of mitochondrion Source: GO_Central
Multicellular organism aging Source: UniProtKB
Regulation of autophagy Source: UniProtKB

Cellular Location

Mitochondrion outer membrane; Endoplasmic reticulum membrane. According to PubMed:20010695, it mainly localizes to the endoplasmic reticulum. However, experiments in mouse showed that it mainly localizes to the mitochondrion outer membrane.

Involvement in disease

Wolfram syndrome 2 (WFS2):
A rare disorder characterized by juvenile-onset insulin-dependent diabetes mellitus with optic atrophy. Other manifestations include diabetes insipidus, sensorineural deafness, dementia, psychiatric illnesses. WFS2 patients additionally show a strong bleeding tendency and gastrointestinal ulceration. Diabetes insipidus may be absent.

Topology

Lumenal: 2-37
Helical: 38-60
Cytoplasmic: 61-135

Kung, W. M., & Lin, M. S. (2021). The NFκB Antagonist CDGSH Iron-Sulfur Domain 2 Is a Promising Target for the Treatment of Neurodegenerative Diseases. International Journal of Molecular Sciences, 22(2), 934.

Kung, W. M., & Lin, M. S. (2021). Beneficial Impacts of Alpha-Eleostearic Acid from Wild Bitter Melon and Curcumin on Promotion of CDGSH Iron-Sulfur Domain 2: Therapeutic Roles in CNS Injuries and Diseases. International Journal of Molecular Sciences, 22(7), 3289.

Shen, Z. Q., Huang, Y. L., Teng, Y. C., Wang, T. W., Kao, C. H., Yeh, C. H., & Tsai, T. F. (2021). CISD2 maintains cellular homeostasis. Biochimica et Biophysica Acta (BBA)-Molecular Cell Research, 118954.

Kung, W. M., Chang, C. J., Chen, T. Y., & Lin, M. S. (2020). Cryogen spray cooling mitigates inflammation and injury-induced CISD2 decline in rat spinal cord hemisection model. Journal of Integrative Neuroscience, 19(4), 619-628.

Nobili, A., Krashia, P., & D'Amelio, M. (2020). Cisd2: a promising new target in Alzheimer's disease. The Journal of pathology, 251(2), 113-116.

Li, J., Duan, H., Xuan, F., Zhao, E., & Huang, M. (2019). CDGSH iron sulfur domain 2 deficiency inhibits cell proliferation and induces cell differentiation of neuroblastoma. Pathology & Oncology Research, 1-9.

Yeh, C. H., Shen, Z. Q., Hsiung, S. Y., Wu, P. C., Teng, Y. C., Chou, Y. J., ... & Tsai, T. F. (2019). Cisd2 is essential to delaying cardiac aging and to maintaining heart functions. PLoS biology, 17(10), e3000508.

Sun, A. G., Meng, F. G., & Wang, M. G. (2017). CISD2 promotes the proliferation of glioma cells via suppressing beclin‑1‑mediated autophagy and is targeted by microRNA‑449a. Molecular medicine reports, 16(6), 7939-7948.

Yang, Y., Bai, Y. S., & Wang, Q. (2017). CDGSH iron sulfur domain 2 activates proliferation and EMT of pancreatic cancer cells via Wnt/β-catenin pathway and has prognostic value in human pancreatic cancer. Oncology research, 25(4), 605.

Ask a question We look forward to hearing from you.

0 reviews or Q&As

Purchasing FAQs
Technical FAQs

Have you used Mouse Anti-CISD2 Recombinant Antibody (569)?
Submit a review and get a Coupon or an Amazon gift card. 20% off Coupon

$30 eGift Card
Submit a review

Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
Other Protocols

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

Online Inquiry

Request bulk or custom quote Second antibody and isotype control

Contact us

Tel: (USA)
(UK)
Fax:

Global Locations

Email:

Online Inquiry