Mouse Anti-CKAP4 Recombinant Antibody (C1949) (CBMAB-C1949-LY)

Name: Mouse Anti-CKAP4 Recombinant Antibody (C1949)
SKU: CBMAB-C1949-LY
Rating: 5 (1 reviews)

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Datasheet

SDS

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Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

C1949

Application

ELISA

Immunogen

CKAP4 of human.

Host Species

Mouse

Specificity

Human

Antibody Isotype

IgG2a, κ

Clonality

Monoclonal Antibody

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS, pH7.4

Preservative

None

Concentration

1 mg/ml

Purity

> 95% Purity determined by SDS-PAGE.

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

More Infomation

Target

Full Name

Cytoskeleton Associated Protein 4

Introduction

CKAP4 (Cytoskeleton Associated Protein 4) is a Protein Coding gene. Diseases associated with CKAP4 include Cystitis and Epispadias. Among its related pathways are Innate Immune System and Protein processing in endoplasmic reticulum. Gene Ontology (GO) annotations related to this gene include signal transducer activity.

Entrez Gene ID

10970

UniProt ID

Q07065

Alternative Names

Cytoskeleton Associated Protein 4; 3-KDa Cytoskeleton-Linking Membrane Protein; Cytoskeleton-Associated Protein 4; CLIMP-63; P63; Transmembrane Protein (63kD), Endoplasmic Reticulum/Golgi Intermediate Compartment; Type-II Transmembrane Protein P63; 3 KDa

Function

Mediates the anchoring of the endoplasmic reticulum to microtubules.

High-affinity epithelial cell surface receptor for the FZD8-related low molecular weight sialoglycopeptide APF/antiproliferative factor. Mediates the APF antiproliferative signaling within cells.

Biological Process

Cellular protein metabolic process Source: Reactome
Neutrophil degranulation Source: Reactome
Post-translational protein modification Source: Reactome

Cellular Location

Endoplasmic reticulum membrane; Cytoskeleton; Cell membrane; Perinuclear region. Translocates to the perinuclear region upon APF-stimulation.

Topology

Cytoplasmic: 1-106
Helical: 107-127
Extracellular: 128-602

PTM

Reversibly palmitoylated. Palmitoylation at Cys-100 by ZDHHC2 is required for its trafficking from the ER to the plasma membrane and for its perinuclear localization. Palmitoylation by ZDHHC2 is also required for its function in APF-mediated antiproliferative signaling (PubMed:19144824).
Increased phosphorylation during mitosis prevents binding to microtubules.

Luo, T., Ding, K., Ji, J., Zhang, X., Yang, X., Chen, A., ... & Li, X. (2021). Cytoskeleton-associated protein 4 (CKAP4) promotes malignant progression of human gliomas through inhibition of the Hippo signaling pathway. Journal of Neuro-Oncology, 154(3), 275-283.

Wang, Y., Yu, H., Xie, X., Deng, T., Ye, L., Wu, L., ... & Chen, G. (2021). Plasmalemma vesicle-associated protein promotes angiogenesis in cholangiocarcinoma via the DKK1/CKAP4/PI3K signaling pathway. Oncogene, 1-14.

Li, S. X., Li, J., Dong, L. W., & Guo, Z. Y. (2020). Cytoskeleton-associated protein 4, a promising biomarker for tumor diagnosis and therapy. Frontiers in Molecular Biosciences, 7.

Chen, Z. Y., Wang, T., Gan, X., Chen, S. H., He, Y. T., Wang, Y. Q., & Zhang, K. H. (2020). Cytoskeleton-associated membrane protein 4 is upregulated in tumor tissues and is associated with clinicopathological characteristics and prognosis in hepatocellular carcinoma. Oncology letters, 19(6), 3889-3898.

Wang, Y., Yu, W., He, M., Huang, Y., Wang, M., & Zhu, J. (2019). Serum cytoskeleton-associated protein 4 as a biomarker for the diagnosis of hepatocellular carcinoma. OncoTargets and therapy, 12, 359.

Chen, L., You, C., Jin, X., Zhou, L., Huang, L., & Wang, Y. (2018). Cytoskeleton-associated protein 4 is a novel serodiagnostic marker for esophageal squamous-cell carcinoma. OncoTargets and therapy, 11, 8221.

Yanagita, K., Nagashio, R., Jiang, S. X., Kuchitsu, Y., Hachimura, K., Ichinoe, M., ... & Sato, Y. (2018). Cytoskeleton-associated protein 4 is a novel serodiagnostic marker for lung cancer. The American journal of pathology, 188(6), 1328-1333.

Gladka, M. M., Molenaar, B., De Ruiter, H., Van Der Elst, S., Tsui, H., Versteeg, D., ... & Van Rooij, E. (2018). Single-cell sequencing of the healthy and diseased heart reveals cytoskeleton-associated protein 4 as a new modulator of fibroblasts activation. Circulation, 138(2), 166-180.

Kikuchi, A., Fumoto, K., & Kimura, H. (2017). The Dickkopf1‐cytoskeleton‐associated protein 4 axis creates a novel signalling pathway and may represent a molecular target for cancer therapy. British journal of pharmacology, 174(24), 4651-4665.

Chavda, B., Ling, J., Majernick, T., & Planey, S. L. (2017). Antiproliferative factor (APF) binds specifically to sites within the cytoskeleton-associated protein 4 (CKAP4) extracellular domain. BMC biochemistry, 18(1), 1-14.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

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