Mouse Anti-CLCN6 Recombinant Antibody (2H2) (CBMAB-C5252-LY)

Name: Mouse Anti-CLCN6 Recombinant Antibody (2H2)
SKU: CBMAB-C5252-LY
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

2H2

Application

ELISA, IF, WB

Immunogen

CLCN6 (770 a.a. ~ 868 a.a) partial recombinant protein.

Host Species

Mouse

Specificity

Human

Antibody Isotype

IgG2b, κ

Clonality

Monoclonal Antibody

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Application	Note
IF(ICC)	10 µg/ml

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS

Preservative

None

Concentration

Batch dependent

Purity

> 95% Purity determined by SDS-PAGE.

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

More Infomation

Target

Full Name

Chloride Voltage-Gated Channel 6

Introduction

This gene encodes a member of the voltage-dependent chloride channel protein family. Members of this family can function as either chloride channels or antiporters. This protein is primarily localized to late endosomes and functions as a chloride/proton antiporter. Alternate splicing results in both coding and non-coding variants. Additional alternately spliced variants have been described but their full-length structure is unknown. [provided by RefSeq, Mar 2012]

Entrez Gene ID

1185

UniProt ID

P51797

Alternative Names

Chloride Voltage-Gated Channel 6; Chloride Channel, Voltage-Sensitive 6; CLC-6; Chloride Transport Protein 6; Chloride Channel Protein 6; Chloride Channel 6; KIAA0046;

Function

Chloride transport protein, initially identified as voltage-gated chloride channel. The presence of the conserved gating glutamate residues suggests that is functions as antiporter.

Biological Process

Cell volume homeostasis Source: UniProtKB
Chloride transport Source: UniProtKB
Ion transmembrane transport Source: Reactome
Response to mechanical stimulus Source: Ensembl
Signal transduction Source: UniProtKB

Cellular Location

Endosome membrane. Detected in detergent-resistant lipid rafts.

Topology

Cytoplasmic: 1-80
Helical: 81-113
Helical: 128-150
Helical: 159-166
Helical: 176-194
Helical: 200-217
Helical: 241-253
Helical: 257-265
Helical: 277-294
Helical: 335-364
Helical: 371-392
Helical: 462-481
Helical: 487-511
Helical: 519-533
Loop between two helices: 534-536
Helical: 537-548
Loop between two helices: 549-552
Helical: 553-571
Cytoplasmic: 572-869

PTM

N-glycosylated on several asparagine residues.

He, H., Cao, X., Yin, F., Wu, T., Stauber, T., & Peng, J. (2021). West syndrome caused by a chloride/proton exchange-uncoupling CLCN6 mutation related to autophagic-lysosomal dysfunction. Molecular Neurobiology, 58(6), 2990-2999.

Polovitskaya, M. M., Barbini, C., Martinelli, D., Harms, F. L., Cole, F. S., Calligari, P., ... & Jentsch, T. J. (2020). A Recurrent Gain-of-Function Mutation in CLCN6, Encoding the ClC-6 Cl−/H+-Exchanger, Causes Early-Onset Neurodegeneration. The American Journal of Human Genetics, 107(6), 1062-1077.

Klemens, C. A., Wu, J., Khan, M. A. H., Levchenko, V., Imig, J. D., Sigmund, C. D., ... & Staruschenko, A. (2020). Abstract P056: Voltage-gated Chloride Channel 6 Regulates Intracellular Calcium Signaling In Vascular Smooth Muscle Cells And Prevents Arterial Stiffening. Hypertension, 76(Suppl_1), AP056-AP056.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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