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Rat Anti-CLDN1 Recombinant Antibody (CBXC-1180) (CBMAB-C1446-CQ)

This product is a rat antibody that recognizes CLDN1. The antibody CBXC-1180 can be used for immunoassay techniques such as: FC.
See all CLDN1 antibodies

Summary

Host Animal
Rat
Specificity
Human
Clone
CBXC-1180
Antibody Isotype
IgG2a
Application
FC

Basic Information

Immunogen
CHO cell line transfected with human Claudin-1, Met1-Val211, Accession # O95832
Specificity
Human
Antibody Isotype
IgG2a
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
A saline solution containing BSA
Preservative
Sodium azide
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
Claudin 1
Introduction
Tight junctions represent one mode of cell-to-cell adhesion in epithelial or endothelial cell sheets, forming continuous seals around cells and serving as a physical barrier to prevent solutes and water from passing freely through the paracellular space. These junctions are comprised of sets of continuous networking strands in the outwardly facing cytoplasmic leaflet, with complementary grooves in the inwardly facing extracytoplasmic leaflet. The protein encoded by this gene, a member of the claudin family, is an integral membrane protein and a component of tight junction strands. Loss of function mutations result in neonatal ichthyosis-sclerosing cholangitis syndrome.
Entrez Gene ID
9076
UniProt ID
O95832
Alternative Names
Claudin 1; Senescence-Associated Epithelial Membrane Protein 1; SEMP1; CLD1; Senescence-Associated Epithelial Membrane Protein; Claudin-1; ILVASC;
Function
Claudins function as major constituents of the tight junction complexes that regulate the permeability of epithelia. While some claudin family members play essential roles in the formation of impermeable barriers, others mediate the permeability to ions and small molecules. Often, several claudin family members are coexpressed and interact with each other, and this determines the overall permeability. CLDN1 is required to prevent the paracellular diffusion of small molecules through tight junctions in the epidermis and is required for the normal barrier function of the skin. Required for normal water homeostasis and to prevent excessive water loss through the skin, probably via an indirect effect on the expression levels of other proteins, since CLDN1 itself seems to be dispensable for water barrier formation in keratinocyte tight junctions (PubMed:23407391).

(Microbial infection) Acts as a co-receptor for hepatitis C virus (HCV) in hepatocytes (PubMed:17325668, PubMed:20375010, PubMed:24038151).

Associates with CD81 and the CLDN1-CD81 receptor complex is essential for HCV entry into host cell (PubMed:20375010).

Acts as a receptor for dengue virus (PubMed:24074594).
Biological Process
Aging Source: Ensembl
Bicellular tight junction assembly Source: UniProtKB
Calcium-independent cell-cell adhesion via plasma membrane cell-adhesion molecules Source: UniProtKB
Cell adhesion Source: GO_Central
Cell-cell junction organization Source: MGI
Cell junction maintenance Source: ARUK-UCL
Cellular response to butyrate Source: Ensembl
Cellular response to interferon-gamma Source: Ensembl
Cellular response to lead ion Source: Ensembl
Cellular response to transforming growth factor beta stimulus Source: Ensembl
Cellular response to tumor necrosis factor Source: Ensembl
Establishment of blood-nerve barrier Source: Ensembl
Establishment of endothelial intestinal barrier Source: Ensembl
Establishment of skin barrier Source: UniProtKB
Hyperosmotic salinity response Source: Ensembl
Liver regeneration Source: Ensembl
Maintenance of blood-brain barrier Source: ARUK-UCL
Positive regulation of bicellular tight junction assembly Source: Ensembl
Positive regulation of cell migration Source: ARUK-UCL
Positive regulation of epithelial cell proliferation involved in wound healing Source: ARUK-UCL
Positive regulation of wound healing Source: ARUK-UCL
Protein complex oligomerization Source: ARUK-UCL
Response to dexamethasone Source: Ensembl
Response to ethanol Source: Ensembl
Response to interleukin-18 Source: Ensembl
Response to lipopolysaccharide Source: Ensembl
Response to toxic substance Source: Ensembl
Xenobiotic transport across blood-nerve barrier Source: Ensembl
Cellular Location
Cell membrane; Basolateral cell membrane; Tight junction. Associates with CD81 and the CLDN1-CD81 complex localizes to the basolateral cell membrane.
Involvement in disease
Ichthyosis-sclerosing cholangitis neonatal syndrome (NISCH):
A rare autosomal recessive complex ichthyosis syndrome characterized by scalp hypotrichosis, scarring alopecia, mild diffuse ichthyosis, sclerosing cholangitis and leukocyte vacuolization.
Topology
Cytoplasmic: 1-7
Helical: 8-28
Extracellular: 29-81
Helical: 82-102
Cytoplasmic: 103-115
Helical: 116-136
Extracellular: 137-163
Helical: 164-184
Cytoplasmic: 185-211

Bhat, A. A., Syed, N., Therachiyil, L., Nisar, S., Hashem, S., Macha, M. A., ... & Haris, M. (2020). Claudin-1, a double-edged sword in cancer. International journal of molecular sciences, 21(2), 569.

Qian, X., Xu, C., Wu, B., Tang, H., Zhao, P., & Qi, Z. (2020). SNORD126 Promotes Hepatitis C Virus Infection by Upregulating Claudin-1 via Activation of PI3K-AKT Signaling Pathway. Frontiers in Microbiology, 11, 2186.

Tong, H., Li, T., Qiu, W., & Zhu, Z. (2019). Claudin‑1 silencing increases sensitivity of liver cancer HepG2 cells to 5‑fluorouracil by inhibiting autophagy. Oncology letters, 18(6), 5709-5716.

Masterson, J. C., Biette, K. A., Hammer, J. A., Nguyen, N., Capocelli, K. E., Saeedi, B. J., ... & Furuta, G. T. (2019). Epithelial HIF-1α/claudin-1 axis regulates barrier dysfunction in eosinophilic esophagitis. The Journal of clinical investigation, 129(8), 3224-3235.

Colpitts, C. C., Tawar, R. G., Mailly, L., Thumann, C., Heydmann, L., Durand, S. C., ... & Baumert, T. F. (2018). Humanisation of a claudin-1-specific monoclonal antibody for clinical prevention and cure of HCV infection without escape. Gut, 67(4), 736-745.

Devriese, S., Eeckhaut, V., Geirnaert, A., Van den Bossche, L., Hindryckx, P., Van de Wiele, T., ... & Laukens, D. (2017). Reduced mucosa-associated Butyricicoccus activity in patients with ulcerative colitis correlates with aberrant claudin-1 expression. Journal of Crohn's and Colitis, 11(2), 229-236.

Cherradi, S., Ayrolles-Torro, A., Vezzo-Vié, N., Gueguinou, N., Denis, V., Combes, E., ... & Del Rio, M. (2017). Antibody targeting of claudin-1 as a potential colorectal cancer therapy. Journal of Experimental & Clinical Cancer Research, 36(1), 1-13.

Moldvay, J., Fábián, K., Jäckel, M., Németh, Z., Bogos, K., Furák, J., ... & Schaff, Z. (2017). Claudin-1 protein expression is a good prognostic factor in non-small cell lung cancer, but only in squamous cell carcinoma cases. Pathology & Oncology Research, 23(1), 151-156.

Hahn-Strömberg, V., Askari, S., Ahmad, A., Befekadu, R., & Nilsson, T. K. (2017). Expression of claudin 1, claudin 4, and claudin 7 in colorectal cancer and its relation with CLDN DNA methylation patterns. Tumor Biology, 39(4), 1010428317697569.

Zheng, G., Fon, G. V., Meixner, W., Creekmore, A., Zong, Y., Dame, M. K., ... & Wiley, J. W. (2017). Chronic stress and intestinal barrier dysfunction: Glucocorticoid receptor and transcription repressor HES1 regulate tight junction protein Claudin-1 promoter. Scientific reports, 7(1), 1-12.

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For research use only. Not intended for any clinical use.

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