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Mouse Anti-CLDN3 Recombinant Antibody (C1056) (V2LY-1206-LY29)


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Summary

Host Animal
Mouse
Specificity
Human
Clone
C1056
Antibody Isotype
IgG2a
Application
FC, IHC, IF

Basic Information

Immunogen
HEK293 human embryonic kidney cell line transfected with human Claudin-3, Met1-Val220.
Host Species
Mouse
Specificity
Human
Antibody Isotype
IgG2a
Clonality
Monoclonal Antibody
Application Notes
ApplicationNote
IHC8-25 µg/ml
IF(ICC)8-25 µg/ml
FC2.5 µg/10^6 cells

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Lyophilized
Buffer
PBS, Trehalose
Preservative
None
Purity
>95% as determined by analysis by SDS-PAGE
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Target

Full Name
Claudin 3
Entrez Gene ID
1365
UniProt ID
O15551
Function
Plays a major role in tight junction-specific obliteration of the intercellular space, through calcium-independent cell-adhesion activity.
Biological Process
Actin cytoskeleton reorganization Source: ARUK-UCL
Bicellular tight junction assembly Source: UniProtKB
Calcium-independent cell-cell adhesion via plasma membrane cell-adhesion molecules Source: Ensembl
Cell adhesion Source: GO_Central
Cell junction maintenance Source: ARUK-UCL
Epithelial cell morphogenesis Source: UniProtKB
Establishment of endothelial blood-brain barrier Source: ARUK-UCL
Maintenance of blood-brain barrier Source: ARUK-UCL
Negative regulation of cell migration Source: ARUK-UCL
Negative regulation of cell population proliferation Source: ARUK-UCL
Negative regulation of gene expression Source: ARUK-UCL
Negative regulation of wound healing Source: ARUK-UCL
Positive regulation of bicellular tight junction assembly Source: ARUK-UCL
Positive regulation of cell junction assembly Source: ARUK-UCL
Positive regulation of cell migration Source: ARUK-UCL
Positive regulation of gene expression Source: ARUK-UCL
Positive regulation of metallopeptidase activity Source: ARUK-UCL
Positive regulation of protein phosphorylation Source: ARUK-UCL
Positive regulation of wound healing Source: ARUK-UCL
Regulation of cell morphogenesis Source: ARUK-UCL
Regulation of membrane permeability Source: ARUK-UCL
Regulation of transepithelial transport Source: ARUK-UCL
Response to ethanol Source: Ensembl
Response to hypoxia Source: UniProtKB
Cellular Location
Cell membrane; Tight junction
Involvement in disease
CLDN3 is located in the Williams-Beuren syndrome (WBS) critical region. WBS results from a hemizygous deletion of several genes on chromosome 7q11.23, thought to arise as a consequence of unequal crossing over between highly homologous low-copy repeat sequences flanking the deleted region.
Topology
Cytoplasmic: 1-8
Helical: 9-29
Extracellular: 30-80
Helical: 81-101
Cytoplasmic: 102-115
Helical: 116-136
Extracellular: 137-159
Helical: 160-180
Cytoplasmic: 181-220
More Infomation

Feng, J., Xu, Y., Wei, Z., Xia, Y., Zhang, H., Shen, C., ... & Fang, Y. (2022). Capsaicin inhibits migration and invasion via inhibiting epithelial-mesenchymal transition in esophageal squamous cell carcinoma by up-regulation of claudin-3 expression. Journal of Functional Foods, 89, 104934.

Anwer, S., Branchard, E., Dan, Q., Dan, A., & Szaszi, K. (2021). Tumor necrosis factor-α induces claudin-3 upregulation in kidney tubular epithelial cells through NF-κB and CREB1. American Journal of Physiology-Cell Physiology, 320(4), C495-C508.

Hempel, C., Protze, J., Altun, E., Riebe, B., Piontek, A., Fromm, A., ... & Piontek, J. (2020). Assembly of tight junction strands: Claudin-10b and claudin-3 form homo-tetrameric building blocks that polymerise in a channel-independent manner. Journal of molecular biology, 432(7), 2405-2427.

Yang, H., Park, H., Lee, Y. J., Choi, J. Y., Kim, T., Rajasekaran, N., ... & Shin, Y. K. (2020). Development of human monoclonal antibody for claudin-3 overexpressing carcinoma targeting. Biomolecules, 10(1), 51.

Yuan, M., Chen, X., Sun, Y., Jiang, L., Xia, Z., Ye, K., ... & He, Q. (2020). ZDHHC12-mediated claudin-3 S-palmitoylation determines ovarian cancer progression. Acta Pharmaceutica Sinica B, 10(8), 1426-1439.

Danzinger, S., Tan, Y. Y., Rudas, M., Kastner, M. T., Weingartshofer, S., Muhr, D., ... & kConFab Investigators. (2018). Differential claudin 3 and EGFR expression predicts BRCA1 mutation in triple-negative breast cancer. Cancer investigation, 36(7), 378-388.

Che, J., Yue, D., Zhang, B., Zhang, H., Huo, Y., Gao, L., ... & Cao, B. (2018). Claudin-3 inhibits lung squamous cell carcinoma cell epithelial-mesenchymal transition and invasion via suppression of the Wnt/β-catenin signaling pathway. International journal of medical sciences, 15(4), 339.

Yamaga, K., Murota, H., Tamura, A., Miyata, H., Ohmi, M., Kikuta, J., ... & Katayama, I. (2018). Claudin-3 loss causes leakage of sweat from the sweat gland to contribute to the pathogenesis of atopic dermatitis. Journal of Investigative Dermatology, 138(6), 1279-1287.

Zhang, L., Wang, Y., Zhang, B., Zhang, H., Zhou, M., Wei, M., ... & Wang, C. (2017). Claudin-3 expression increases the malignant potential of lung adenocarcinoma cells: role of epidermal growth factor receptor activation. Oncotarget, 8(14), 23033.

Worst, T. S., Von Hardenberg, J., Gross, J. C., Erben, P., Schnölzer, M., Hausser, I., ... & Boutros, M. (2017). Database-augmented mass spectrometry analysis of exosomes identifies claudin 3 as a putative prostate cancer biomarker. Molecular & Cellular Proteomics, 16(6), 998-1008.

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For research use only. Not intended for any clinical use.

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