Human Anti-CLDN4 Recombinant Antibody (
8G7) (V2LY-0725-LY667)

Name: Human Anti-CLDN4 Recombinant Antibody (8G7)
SKU: V2LY-0725-LY667
Rating: 5 (1 reviews)

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Tested Data

Human Anti-CLDN4 Recombinant Antibody (8G7) (V2LY-0725-LY667) in ELISA

The Binding Activity of Human CLDN4 with Anti-CLDN4 Recombinant AntibodyActivity: Measured by its binding ability in a functional ELISA. Immobilized Human CLDN4 at 5 μg/mL can bind Anti-CLDN4 recombinant antibody, the EC50 is 29.56-50.75 ng/mL.

Datasheet

SDS

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Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Human

Clone

8G7

Application

ELISA

Immunogen

Recombinant Human CLDN4 protein.

Host Species

Human

Specificity

Human

Antibody Isotype

IgG1

Clonality

Monoclonal Antibody

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS, Glycerol

Preservative

Sodium azide

Concentration

Batch dependent

Purity

> 95% Purity determined by SDS-PAGE.

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

More Infomation

Target

Full Name

Claudin 4

Entrez Gene ID

UniProt ID

P68133

Function

Channel-forming tight junction protein that mediates paracellular chloride transport in the kidney. Plays a critical role in the paracellular reabsorption of filtered chloride in the kidney collecting ducts. Claudins play a major role in tight junction-specific obliteration of the intercellular space, through calcium-independent cell-adhesion activity.

Biological Process

Bicellular tight junction assembly Source: GO_Central
Calcium-independent cell-cell adhesion via plasma membrane cell-adhesion molecules Source: UniProtKB
Cell adhesion Source: GO_Central
Chloride transport Source: UniProtKB-KW
Circadian rhythm Source: Ensembl
Establishment of skin barrier Source: UniProtKB
Female pregnancy Source: Ensembl
Positive regulation of cell migration Source: ARUK-UCL
Positive regulation of metallopeptidase activity Source: ARUK-UCL
Positive regulation of wound healing Source: ARUK-UCL
Regulation of cell morphogenesis Source: ARUK-UCL
Renal absorption Source: UniProtKB
Response to progesterone Source: Ensembl

Cellular Location

Cell membrane; Tight junction. CLDN4 is required for tight junction localization in the kidney.

Involvement in disease

CLDN4 is located in the Williams-Beuren syndrome (WBS) critical region. WBS results from a hemizygous deletion of several genes on chromosome 7q11.23, thought to arise as a consequence of unequal crossing over between highly homologous low-copy repeat sequences flanking the deleted region.

Topology

Cytoplasmic: 1-7
Helical: 8-28
Extracellular: 29-81
Helical: 82-102
Cytoplasmic: 103-117
Helical: 118-138
Extracellular: 139-160
Helical: 161-181
Cytoplasmic: 182-209

PTM

Phosphorylated. Phosphorylation by EPHA2 is stimulated by EFNA1 and alters interaction with TJP1.

Li, H., Neelankal John, A., Nagatake, T., Hamazaki, Y., & Jiang, F. X. (2020). Claudin 4 in pancreatic β cells is involved in regulating the functional state of adult islets. FEBS open bio, 10(1), 28-40.

Rambabu, M., & Jayanthi, S. (2020). Screening approaches against claudin-4 focusing on therapeutics through molecular docking and the analysis of their relative dynamics: a theoretical approach. Journal of Receptors and Signal Transduction, 40(5), 436-441.

Luo, Y., Kishi, S., Sasaki, T., Ohmori, H., Fujiwara‐Tani, R., Mori, S., ... & Kuniyasu, H. (2020). Targeting claudin‐4 enhances chemosensitivity in breast cancer. Cancer science, 111(5), 1840.

Nakashima, C., Yamamoto, K., Kishi, S., Sasaki, T., Ohmori, H., Fujiwara-Tani, R., ... & Kuniyasu, H. (2020). Clostridium perfringens enterotoxin induces claudin-4 to activate YAP in oral squamous cell carcinomas. Oncotarget, 11(4), 309.

Owari, T., Sasaki, T., Fujii, K., Fujiwara-Tani, R., Kishi, S., Mori, S., ... & Kuniyasu, H. (2020). Role of Nuclear Claudin-4 in Renal Cell Carcinoma. International Journal of Molecular Sciences, 21(21), 8340.

Ding, G., Liu, J., Shao, Q., Wang, B., Feng, J., Li, Y., ... & Xiao, Y. (2020). Porcine reproductive and respiratory syndrome virus structural protein GP3 regulates claudin 4 to facilitate the early stages of infection. Journal of Virology, 94(20), e00124-20.

Nishiguchi, Y., Fujiwara-Tani, R., Sasaki, T., Luo, Y., Ohmori, H., Kishi, S., ... & Kuniyasu, H. (2019). Targeting claudin-4 enhances CDDP-chemosensitivity in gastric cancer. Oncotarget, 10(22), 2189.

Rambabu, M., & Jayanthi, S. (2019). Virtual screening of National Cancer Institute database for claudin‐4 inhibitors: Synthesis, biological evaluation, and molecular dynamics studies. Journal of cellular biochemistry, 120(5), 8588-8600.

Sasaki, T., Fujiwara‐Tani, R., Kishi, S., Mori, S., Luo, Y., Ohmori, H., ... & Kuniyasu, H. (2019). Targeting claudin‐4 enhances chemosensitivity of pancreatic ductal carcinomas. Cancer medicine, 8(15), 6700-6708.

Fujiwara-Tani, R., Sasaki, T., Luo, Y. I., Goto, K., Kawahara, I., Nishiguchi, Y., ... & Kuniyasu, H. (2018). Anti-claudin-4 extracellular domain antibody enhances the antitumoral effects of chemotherapeutic and antibody drugs in colorectal cancer. Oncotarget, 9(100), 37367.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

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