What is the advantage of using a human recombinant antibody to study human CLDN6?

A human recombinant antibody is engineered to have a human antibody sequence. When studying a human protein like CLDN6, using a human antibody can offer several advantages. It may exhibit more native binding characteristics in human biological systems, potentially leading to reduced off-target binding and lower background in human cell lines or tissues. Additionally, for certain functional studies or potential future therapeutic development, a human antibody format is often preferred.

What are the recommended storage conditions for this recombinant antibody?

For long-term preservation of the Human Anti-CLDN6 Recombinant Antibody (C0999), storage at -20°C or colder is recommended. For typical short-term use (a few weeks), the antibody can usually be stored at 2-8°C. 1 It is critical to avoid repeated freeze-thaw cycles as this can compromise antibody integrity and performance. 2 Aliquoting the antibody upon receipt is a good practice to minimize the impact of freezing and thawing on the entire stock.

Human Anti-CLDN6 Recombinant Antibody (C0999) (CBMAB-C0999-LY)

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Datasheet

SDS

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Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Human

Clone

C0999

Application

ELISA, FC

Immunogen

Human Claudin 6.

Host Species

Human

Specificity

Human

Antibody Isotype

IgG1

Clonality

Monoclonal Antibody

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Application	Note
ELISA	1:5,000-10,000
FC	1:100

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Lyophilized

Buffer

PBS, Trehalose

Preservative

None

Concentration

0.2 mg/ml

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

More Infomation

Target

Full Name

Claudin 6

Introduction

Tight junctions represent one mode of cell-to-cell adhesion in epithelial or endothelial cell sheets, forming continuous seals around cells and serving as a physical barrier to prevent solutes and water from passing freely through the paracellular space. These junctions are comprised of sets of continuous networking strands in the outwardly facing cytoplasmic leaflet, with complementary grooves in the inwardly facing extracytoplasmic leaflet. This gene encodes a component of tight junction strands, which is a member of the claudin family. The protein is an integral membrane protein and is one of the entry cofactors for hepatitis C virus. The gene methylation may be involved in esophageal tumorigenesis. This gene is adjacent to another family member CLDN9 on chromosome 16.[provided by RefSeq, Aug 2010]

Entrez Gene ID

9074

UniProt ID

P56747

Alternative Names

Claudin 6; Skullin; Claudin-6;

Function

Plays a major role in tight junction-specific obliteration of the intercellular space.

(Microbial infection) Acts as a receptor for hepatitis C virus (HCV) entry into hepatic cells.

Biological Process

Bicellular tight junction assembly Source: GO_Central
Calcium-independent cell-cell adhesion via plasma membrane cell-adhesion molecules Source: UniProtKB
Cell adhesion Source: GO_Central

Cellular Location

Cell membrane; Tight junction

Topology

Cytoplasmic: 1-7
Helical: 8-28
Extracellular: 29-81
Helical: 82-102
Cytoplasmic: 103-116
Helical: 117-137
Extracellular: 138-160
Helical: 161-181
Cytoplasmic: 182-220

Matsuzaki, J., Lele, S., Odunsi, K., & Tsuji, T. (2022). Identification of Claudin 6-specific HLA class I-and HLA class II-restricted T cell receptors for cellular immunotherapy in ovarian cancer. Oncoimmunology, 11(1), 2020983.

Du, H., Yang, X., Fan, J., & Du, X. (2021). Claudin 6: Therapeutic prospects for tumours, and mechanisms of expression and regulation. Molecular Medicine Reports, 24(3), 1-9.

Gao, P., Peng, T., Cao, C., Lin, S., Wu, P., Huang, X., ... & Wu, P. (2021). Association of CLDN6 and CLDN10 With Immune Microenvironment in Ovarian Cancer: A Study of the Claudin Family. Frontiers in Genetics, 12.

Kojima, M., Sugimoto, K., Tanaka, M., Endo, Y., Kato, H., Honda, T., ... & Chiba, H. (2020). Prognostic significance of aberrant claudin-6 expression in endometrial cancer. Cancers, 12(10), 2748.

Kojima, M., Sugimoto, K., Kobayashi, M., Ichikawa-Tomikawa, N., Kashiwagi, K., Watanabe, T., ... & Chiba, H. (2021). Aberrant Claudin-6–Adhesion Signaling Promotes Endometrial Cancer Progression via Estrogen Receptor α. Molecular Cancer Research.

Kohmoto, T., Masuda, K., Shoda, K., Takahashi, R., Ujiro, S., Tange, S., ... & Imoto, I. (2020). Claudin-6 is a single prognostic marker and functions as a tumor-promoting gene in a subgroup of intestinal type gastric cancer. Gastric Cancer, 23(3), 403-417.

Lewis, J. B., Bodine, J. S., Gassman, J. R., Muñoz, S. A., Milner, D. C., Dunaway, T. M., ... & Reynolds, P. R. (2018). Transgenic up-regulation of Claudin-6 decreases fine diesel particulate matter (DPM)-induced pulmonary inflammation. Environmental Science and Pollution Research, 25(18), 18179-18188.

Lin, D., Guo, Y., Li, Y., Ruan, Y., Zhang, M., Jin, X., ... & Quan, C. (2017). Bioinformatic analysis reveals potential properties of human Claudin-6 regulation and functions. Oncology reports, 38(2), 875-885.

Bekki, H., Yamamoto, H., Takizawa, K., Iwasaki, T., Otsuka, H., Yamada, Y., ... & Oda, Y. (2017). Claudin 6 expression is useful to distinguish myxofibrosarcomas from other myxoid soft tissue tumors. Pathology-Research and Practice, 213(6), 674-679.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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