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Mouse Anti-CLEC4E Recombinant Antibody (16E3) (V2LY-1206-LY145)


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Summary

Host Animal
Mouse
Specificity
Human, Rat
Clone
16E3
Antibody Isotype
IgG2b, κ
Application
ELISA, IF, FC, IHC-P, WB

Basic Information

Immunogen
Recombinant human CLEC4E (41-219aa) purified from E. coli.
Host Species
Mouse
Specificity
Human, Rat
Antibody Isotype
IgG2b, κ
Clonality
Monoclonal Antibody
Application Notes
ApplicationNote
WB1:250-1:500
ELISA1:100-1:2,000
FC1:10-1:1,000
IF(ICC)1:250-1:500
IHC-P1:50

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
PBS, 10% Glycerol
Preservative
0.02% sodium azide
Concentration
1 mg/ml
Purity
>95% as determined by analysis by SDS-PAGE
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Target

Full Name
C-Type Lectin Domain Family 4 Member E
Entrez Gene ID
Human26253
Rat450223
UniProt ID
HumanQ9ULY5
RatQ67EQ1
Function
Calcium-dependent lectin that acts as a pattern recognition receptor (PRR) of the innate immune system: recognizes damage-associated molecular patterns (DAMPs) of abnormal self and pathogen-associated molecular patterns (PAMPs) of bacteria and fungi (PubMed:18509109, PubMed:23602766).

The PAMPs notably include mycobacterial trehalose 6,6'-dimycolate (TDM), a cell wall glycolipid with potent adjuvant immunomodulatory functions (PubMed:23602766, PubMed:24101491).

Interacts with signaling adapter Fc receptor gamma chain/FCER1G to form a functional complex in myeloid cells (By similarity).

Binding of mycobacterial trehalose 6,6'-dimycolate (TDM) to this receptor complex leads to phosphorylation of the immunoreceptor tyrosine-based activation motif (ITAM) of FCER1G, triggering activation of SYK, CARD9 and NF-kappa-B, consequently driving maturation of antigen-presenting cells and shaping antigen-specific priming of T-cells toward effector T-helper 1 and T-helper 17 cell subtypes (By similarity).

Also recognizes alpha-mannose residues on pathogenic fungi of the genus Malassezia and mediates macrophage activation (By similarity).

Through recognition of DAMPs released upon nonhomeostatic cell death, enables immune sensing of damaged self and promotes inflammatory cell infiltration into the damaged tissue (By similarity).
Biological Process
Innate immune response Source: UniProtKB-KW
Pattern recognition receptor signaling pathway Source: UniProtKB
Stimulatory C-type lectin receptor signaling pathway Source: Reactome
Cellular Location
Cell membrane; Phagocytic cup
Topology
Cytoplasmic: 1-19
Helical: 20-40
Extracellular: 41-219
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For research use only. Not intended for any clinical use.

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