Mouse Anti-CLGN Recombinant Antibody (CBXC-0470) (V2LY-1206-LY211)

Name: Mouse Anti-CLGN Recombinant Antibody (CBXC-0470)
SKU: V2LY-1206-LY211
Rating: 5 (1 reviews)

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Datasheet

SDS

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Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

CBXC-0470

Application

WB, IP, IF, ELISA

Immunogen

Amino acids 507-610 mapping at the C-terminus of Calmegin of human.

Host Species

Mouse

Specificity

Human

Antibody Isotype

IgG2a, κ

Clonality

Monoclonal Antibody

Application Notes

Application	Note
WB	1:100-1:1,000
IP	1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)
IF(ICC)	1:50-1:500
ELISA	1:100-1:1,000

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

0.1% gelatin

Preservative

<0.1% sodium azide

Concentration

0.2 mg/ml

Purity

>95% as determined by analysis by SDS-PAGE

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

More Infomation

Target

Full Name

calmegin

Entrez Gene ID

1047

UniProt ID

O14967

Function

Functions during spermatogenesis as a chaperone for a range of client proteins that are important for sperm adhesion onto the egg zona pellucida and for subsequent penetration of the zona pellucida. Required for normal sperm migration from the uterus into the oviduct. Required for normal male fertility. Binds calcium ions (By similarity).

Biological Process

Binding of sperm to zona pellucida Source: Ensembl
Endoplasmic reticulum unfolded protein response Source: GO_Central
Protein-containing complex assembly Source: Ensembl
Protein folding Source: GO_Central
Single fertilization Source: ProtInc

Cellular Location

Endoplasmic reticulum membrane

Topology

Lumenal: 20-471
Helical: 472-492
Cytoplasmic: 493-610

Ganoza Reyes, L. (2021). Diseño y validación de primers para el gen Calmegin (CLGN) en alpacas.

Itcho, K., Oki, K., Gomez-Sanchez, C. E., Gomez-Sanchez, E. P., Ohno, H., Kobuke, K., ... & Yoneda, M. (2020). Endoplasmic reticulum chaperone calmegin is upregulated in aldosterone-producing adenoma and associates with aldosterone production. Hypertension, 75(2), 492-499.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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