Mouse Anti-CLTA Recombinant Antibody (C0587) (CBMAB-C0587-CQ)

Name: Mouse Anti-CLTA Recombinant Antibody (C0587)
SKU: CBMAB-C0587-CQ
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

C0587

Application

IHC-P

Immunogen

Purified recombinant N-terminal fragment of human Clathrin Light Chain.

Host Species

Mouse

Specificity

Human

Antibody Isotype

IgG2b, κ

Clonality

Monoclonal Antibody

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Application	Note
IHC-P	1-2 μg/ml

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS, 0.05% BSA

Preservative

0.05% sodium azide

Concentration

0.2 mg/ml

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

More Infomation

Target

Full Name

clathrin, light chain (Lca)

Introduction

Clathrin is a large, soluble protein composed of heavy and light chains. It functions as the main structural component of the lattice-type cytoplasmic face of coated pits and vesicles which entrap specific macromolecules during receptor-mediated endocytosis. This gene encodes one of two clathrin light chain proteins which are believed to function as regulatory elements. Alternative splicing results in multiple transcript variants. Related pseudogenes have been identified on chromosomes 8 and 12.

Entrez Gene ID

1211

UniProt ID

P09496

Alternative Names

Clathrin Light Chain A; Clathrin, Light Polypeptide (Lca); LCA; Clathrin, Light Chain A;

Function

Clathrin is the major protein of the polyhedral coat of coated pits and vesicles. Acts as component of the TACC3/ch-TOG/clathrin complex proposed to contribute to stabilization of kinetochore fibers of the mitotic spindle by acting as inter-microtubule bridge (PubMed:15858577, PubMed:21297582).

Biological Process

Antigen processing and presentation of exogenous peptide antigen via MHC class II Source: Reactome
Cell cycle Source: UniProtKB-KW
Cell division Source: UniProtKB-KW
Clathrin coat assembly Source: CAFA
Clathrin-dependent endocytosis Source: GO_Central
Intracellular protein transport Source: InterPro
Low-density lipoprotein particle clearance Source: Reactome
Low-density lipoprotein particle receptor catabolic process Source: Reactome
Membrane organization Source: Reactome
Post-Golgi vesicle-mediated transport Source: Reactome

Cellular Location

Spindle; Cytoplasmic vesicle membrane; Coated pit. Cytoplasmic face of coated pits and vesicles. In complex with TACC3 and CKAP5 (forming the TACC3/ch-TOG/clathrin complex) localized to inter-microtubule bridges in mitotic spindles.

Redlingshöfer, L., McLeod, F., Chen, Y., Camus, M. D., Burden, J. J., Palomer, E., ... & Brodsky, F. M. (2020). Clathrin light chain diversity regulates membrane deformation in vitro and synaptic vesicle formation in vivo. Proceedings of the National Academy of Sciences, 117(38), 23527-23538.

Tsygankova, O. M., & Keen, J. H. (2019). A unique role for clathrin light chain A in cell spreading and migration. Journal of cell science, 132(10), jcs224030.

Biancospino, M., Buel, G. R., Niño, C. A., Maspero, E., Scotto di Perrotolo, R., Raimondi, A., ... & Polo, S. (2019). Clathrin light chain A drives selective myosin VI recruitment to clathrin-coated pits under membrane tension. Nature communications, 10(1), 1-15.

Anderson, R. H., Kerkvliet, J. G., Otta, J. J., Ross, A. D., Leiferman, P. C., Hoppe, A. D., & Francis, K. R. (2018). Generation of a CLTA reporter human induced pluripotent stem cell line, CRMi001-A-1, using the CRISPR/Cas9 system to monitor endogenous clathrin trafficking. Stem cell research, 33, 95-99.

Lu, Y., Wang, J., Guo, X., Yan, S., & Dai, J. (2017). Perfluorooctanoic acid affects endocytosis involving clathrin light chain A and microRNA-133b-3p in mouse testes. Toxicology and Applied Pharmacology, 318, 41-48.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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