Mouse Anti-CLTC Recombinant Antibody (CBCNC-216) (CBMAB-C2488-CN)

Name: Mouse Anti-CLTC Recombinant Antibody (CBCNC-216)
SKU: CBMAB-C2488-CN
Rating: 5 (1 reviews)

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Datasheet

SDS

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Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

CBCNC-216

Application

ELISA

Immunogen

Purified recombinant N-terminal fragment of human Clathrin Heavy Chain 1/CHC17.

Host Species

Mouse

Specificity

Human

Antibody Isotype

IgG1, κ

Clonality

Monoclonal Antibody

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS, 0.05% BSA

Preservative

0.05% sodium azide

Concentration

0.2 mg/ml

More Infomation

Target

Full Name

Clathrin Heavy Chain

Introduction

Clathrin is a major protein component of the cytoplasmic face of intracellular organelles, called coated vesicles and coated pits. These specialized organelles are involved in the intracellular trafficking of receptors and endocytosis of a variety of macromolecules. The basic subunit of the clathrin coat is composed of three heavy chains and three light chains. [provided by RefSeq, Jul 2008]

Entrez Gene ID

281080

UniProt ID

P49951

Alternative Names

Clathrin Heavy Chain; Clathrin Heavy Chain On Chromosome 17; Clathrin, Heavy Polypeptide-Like 2; Clathrin, Heavy Polypeptide (Hc); CLTCL2; CLH-17; Clathrin, Heavy Chain (Hc); Clathrin Heavy Chain 1;

Function

Clathrin is the major protein of the polyhedral coat of coated pits and vesicles. Two different adapter protein complexes link the clathrin lattice either to the plasma membrane or to the trans-Golgi network. Acts as component of the TACC3/ch-TOG/clathrin complex proposed to contribute to stabilization of kinetochore fibers of the mitotic spindle by acting as inter-microtubule bridge (PubMed:15858577, PubMed:16968737, PubMed:21297582).

The TACC3/ch-TOG/clathrin complex is required for the maintenance of kinetochore fiber tension (PubMed:23532825).

Plays a role in early autophagosome formation (PubMed:20639872).

Biological Process

Amyloid-beta clearance by transcytosis Source: ARUK-UCL
Antigen processing and presentation of exogenous peptide antigen via MHC class II Source: Reactome
Autophagy Source: UniProtKB-KW
Cell division Source: UniProtKB-KW
Clathrin coat assembly Source: CAFA
Clathrin-dependent endocytosis Source: ARUK-UCL
Intracellular protein transport Source: UniProtKB
Low-density lipoprotein particle clearance Source: Reactome
Low-density lipoprotein particle receptor catabolic process Source: Reactome
Membrane organization Source: Reactome
Mitotic cell cycle Source: UniProtKB
Negative regulation of hyaluronan biosynthetic process Source: UniProtKB
Negative regulation of protein localization to plasma membrane Source: UniProtKB
Osteoblast differentiation Source: UniProtKB
Post-Golgi vesicle-mediated transport Source: Reactome
Receptor internalization Source: BHF-UCL
Receptor-mediated endocytosis Source: UniProtKB
Regulation of mitotic spindle organization Source: UniProtKB
Retrograde transport, endosome to Golgi Source: UniProtKB
Transferrin transport Source: BHF-UCL
Wnt signaling pathway, planar cell polarity pathway Source: Reactome

Cellular Location

Spindle; Cytoplasmic vesicle membrane; Coated pit; Melanosome. Cytoplasmic face of coated pits and vesicles. Identified by mass spectrometry in melanosome fractions from stage I to stage IV. In complex with TACC3 and CKAP5 (forming the TACC3/ch-TOG/clathrin complex) localized to inter-microtubule bridges in mitotic spindles.

Involvement in disease

Mental retardation, autosomal dominant 56 (MRD56):
A form of mental retardation, a disorder characterized by significantly below average general intellectual functioning associated with impairments in adaptive behavior and manifested during the developmental period.

Itai, T., Miyatake, S., Tsuchida, N., Saida, K., Narahara, S., Tsuyusaki, Y., ... & Matsumoto, N. (2022). Novel CLTC variants cause new brain and kidney phenotypes. Journal of Human Genetics, 67(1), 1-7.

Bridge, J. A., Sumegi, J., Royce, T., Baker, M., & Linos, K. (2021). A novel CLTC‐FOSB gene fusion in pseudomyogenic hemangioendothelioma of bone. Genes, Chromosomes and Cancer, 60(1), 38-42.

Nabais Sá, M. J., Venselaar, H., Wiel, L., Trimouille, A., Lasseaux, E., Naudion, S., ... & Koolen, D. A. (2020). De novo CLTC variants are associated with a variable phenotype from mild to severe intellectual disability, microcephaly, hypoplasia of the corpus callosum, and epilepsy. Genetics in Medicine, 22(4), 797-802.

Manti, F., Nardecchia, F., Barresi, S., Venditti, M., Pizzi, S., Hamdan, F. F., ... & Leuzzi, V. (2019). Neurotransmitter trafficking defect in a patient with clathrin (CLTC) variation presenting with intellectual disability and early-onset parkinsonism. Parkinsonism & Related Disorders, 61, 207-210.

Anderson, R. H., Kerkvliet, J. G., Otta, J. J., Ross, A. D., Leiferman, P. C., Hoppe, A. D., & Francis, K. R. (2018). Generation of a CLTA reporter human induced pluripotent stem cell line, CRMi001-A-1, using the CRISPR/Cas9 system to monitor endogenous clathrin trafficking. Stem cell research, 33, 95-99.

Latomanski, E. A., & Newton, H. J. (2018). Interaction between autophagic vesicles and the Coxiella-containing vacuole requires CLTC (clathrin heavy chain). Autophagy, 14(10), 1710-1725.

Lin, M. H., Liu, Y. C., Liu, S. Y., Chen, F. C., Yang, P. J., Li, G. H., ... & Yen, C. Y. (2018). Clathrin‐mediated endocytosis is required for ANE 30‐100K‐induced autophagy. Journal of Oral Pathology & Medicine, 47(1), 25-31.

Mizuta, H., Mushirobira, Y., Nagata, J., Todo, T., Hara, A., Reading, B. J., ... & Hiramatsu, N. (2017). Ovarian expression and localization of clathrin (Cltc) components in cutthroat trout, Oncorhynchus clarki: evidence for Cltc involvement in endocytosis of vitellogenin during oocyte growth. Comparative Biochemistry and Physiology Part A: Molecular & Integrative Physiology, 212, 24-34.

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Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
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Isotype control

For research use only. Not intended for any clinical use.

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