Mouse Anti-CNOT10 Recombinant Antibody (CBWJC-2862) (V2LY-1206-LY451)

Name: Mouse Anti-CNOT10 Recombinant Antibody (CBWJC-2862)
SKU: V2LY-1206-LY451
Rating: 5 (1 reviews)

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Datasheet

SDS

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Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

CBWJC-2862

Application

WB, IP, IF, ELISA

Immunogen

Amino acids 302-468 mapping within an internal region of CNOT10 of human.

Host Species

Mouse

Specificity

Mouse, Rat, Human

Antibody Isotype

IgG1, κ

Clonality

Monoclonal Antibody

Application Notes

Application	Note
WB	1:100-1:1,000
IP	1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)
IF(ICC)	1:50-1:500
ELISA	1:100-1:1,000

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

0.1% gelatin

Preservative

<0.1% sodium azide

Concentration

0.2 mg/ml

Purity

>95% as determined by analysis by SDS-PAGE

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

More Infomation

Target

Full Name

CNOT10

Entrez Gene ID

Human	25904
Mouse	78893
Rat	316034

UniProt ID

Human	Q9H9A5
Mouse	Q8BH15
Rat	Q5XIA4

Function

Component of the CCR4-NOT complex which is one of the major cellular mRNA deadenylases and is linked to various cellular processes including bulk mRNA degradation, miRNA-mediated repression, translational repression during translational initiation and general transcription regulation. Additional complex functions may be a consequence of its influence on mRNA expression. Is not required for association of CNOT7 to the CCR4-NOT complex.

Biological Process

DNA damage response, signal transduction by p53 class mediator resulting in cell cycle arrest Source: Reactome
Gene silencing by RNA Source: UniProtKB-KW
mRNA catabolic process Source: GO_Central
Negative regulation of translation Source: GO_Central
Nuclear-transcribed mRNA poly(A) tail shortening Source: Reactome

Cellular Location

Nucleus; Cytoplasm

Du, H., Chen, C., Wang, Y., Yang, Y., Che, Z., Liu, X., ... & Luo, Z. (2020). RNF219 interacts with CCR4–NOT in regulating stem cell differentiation. Journal of molecular cell biology, 12(11), 894-905.

Zhou, H. R., Lin, R. N., Huang, H. W., Li, L., Cai, T., Zhu, J. K., ... & He, X. J. (2020). The CCR4‐NOT complex component NOT1 regulates rna‐directed DNA methylation and transcriptional silencing by facilitating pol iv‐dependent siRNA production. The Plant Journal, 103(4), 1503-1515.

Raisch, T., Chang, C. T., Levdansky, Y., Muthukumar, S., Raunser, S., & Valkov, E. (2019). Reconstitution of recombinant human CCR4-NOT reveals molecular insights into regulated deadenylation. Nature communications, 10(1), 1-14.

Bulbrook, D., Brazier, H., Mahajan, P., Kliszczak, M., Fedorov, O., Marchese, F. P., ... & Dean, J. L. E. (2018). Tryptophan-mediated interactions between tristetraprolin and the CNOT9 subunit are required for CCR4-NOT deadenylase complex recruitment. Journal of molecular biology, 430(5), 722-736.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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