Rabbit Anti-CNOT8 Recombinant Antibody (CBFYC-1999) (V2LY-1206-LY480)

Name: Rabbit Anti-CNOT8 Recombinant Antibody (CBFYC-1999)
SKU: V2LY-1206-LY480
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Rabbit

Clone

CBFYC-1999

Application

WB, IF

Immunogen

Recombinant fragment of human CNOT8.

Host Species

Rabbit

Specificity

Human, Mouse, Rat, Zebrafish

Antibody Isotype

IgG

Clonality

Monoclonal Antibody

Application Notes

Application	Note
WB	1:500-1:10,000

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS

Preservative

None

Concentration

1 mg/ml

Purity

>95% as determined by analysis by SDS-PAGE

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

More Infomation

Target

Full Name

CCR4-NOT transcription complex, subunit 8

Entrez Gene ID

Human	9337
Mouse	69125
Rat	363603

UniProt ID

Human	Q9UFF9
Mouse	Q9D8X5
Rat	A6HEQ7

Function

Has 3'-5' poly(A) exoribonuclease activity for synthetic poly(A) RNA substrate. Its function seems to be partially redundant with that of CNOT7. Catalytic component of the CCR4-NOT complex which is linked to various cellular processes including bulk mRNA degradation, miRNA-mediated repression, translational repression during translational initiation and general transcription regulation. During miRNA-mediated repression the complex seems also to act as translational repressor during translational initiation. Additional complex functions may be a consequence of its influence on mRNA expression. Associates with members of the BTG family such as TOB1 and BTG2 and is required for their anti-proliferative activity.

Biological Process

DNA damage response, signal transduction by p53 class mediator resulting in cell cycle arrest Source: Reactome
Exonucleolytic catabolism of deadenylated mRNA Source: UniProtKB
Gene silencing by miRNA Source: UniProtKB
Negative regulation of translation Source: GO_Central
Nuclear-transcribed mRNA poly(A) tail shortening Source: Reactome
Positive regulation of cell population proliferation Source: UniProtKB
Positive regulation of mRNA catabolic process Source: BHF-UCL
Transcription, DNA-templated Source: InterPro

Cellular Location

Nucleus; Cytoplasm

Mostafa, D., Takahashi, A., Yanagiya, A., Yamaguchi, T., Abe, T., Kureha, T., ... & Suzuki, T. (2020). Essential functions of the CNOT7/8 catalytic subunits of the CCR4-NOT complex in mRNA regulation and cell viability. RNA biology, 17(3), 403-416.

Eskandarian, S., Grand, R., Irani, S., Saeedi, M., & Mirfakhraie, R. (2020). Importance of CNOT8 Deadenylase Subunit in DNA Damage Responses Following Ionizing Radiation (IR). Reports of Biochemistry & Molecular Biology, 9(2), 163.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Related Products

Mouse Anti-CNOT8 Recombinant Antibody (1F11) (CAT#: V2LY-1206-LY479)

Isotype control

For research use only. Not intended for any clinical use.

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