Mouse Anti-COL6A2 Recombinant Antibody (2C5-F2) (V2LY-1206-LY697)

Name: Mouse Anti-COL6A2 Recombinant Antibody (2C5-F2)
SKU: V2LY-1206-LY697
Rating: 5 (1 reviews)

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Published Data

Mouse Anti-COL6A2 Recombinant Antibody (2C5-F2) (V2LY-1206-LY697) in IHC

Immunohistochemical analysis of selected proteins. Representative images were taken. Aβ pathology was visualized. a The control case does not have any Aβ pathology. b Plaque pathology is confirmed in the AD case and (c) CAA type-1 pathology is confirmed in the CAA type-1 case. d-f Extensive NDP immunoreactivity is observed in the CAA type-1 cases whereas absent in both control and AD cases without CAA. g-i COL6A2 immunoreactivity is hardly observed in the control and AD cases, however, extensive immunoreactivity is observed in the CAA type cases and includes both capillaries and large vessels. j-l Immunoreactivity for HTRA1 is absent in control tissue, however, is observed both related to plaque pathology and CAA at comparable intensity. m-o Immunoreactivity for APCS is absent in control cases but is observed both related to plaque and CAA type-1 pathology. However, the intensity of the staining observed in the AD cases is considerably less. p-r Also, APOE immunoreactivity is observed related to both plaque and CAA type-1 pathology, yet its intensity in CAA type-1 is far greater. Scale bar, 100 μm in images A to R. Scale bar in image (C`) 10 μm. ...View More

Hondius, D. C., Eigenhuis, K. N., Morrema, T. H., van der Schors, R. C., van Nierop, P., Bugiani, M., ... & Rozemuller, A. J. (2018). Proteomics analysis identifies new markers associated with capillary cerebral amyloid angiopathy in Alzheimer's disease. Acta Neuropathologica Communications, 6, 1-19.

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Mouse Anti-COL6A2 Recombinant Antibody (2C5-F2) (V2LY-1206-LY697) in IHC

Expression of CAA type-1 markers in other small vessel diseases. IHC for Aβ, NDP, COL6A2, APCS and APOE was performed. In a CAA type-1 case immunoreactivity for all marker proteins is confirmed (a-e). Also immunoreactivity is seen for all markers in the cotton wool case and Aβ pathology was confirmed (f). For NDP, APOE and APCS immunoreactivity is also seen localizing to severe dyshoric angiopathy (g, i and j). COL6A2 immunoreactivity is restricted to the vessel wall (h). In de Prp-CAA case Aβ pathology was absent (k). Extensive immunoreactivity was observed for NDP, COL6A2, APOE and APCS (l-o). No immunoreactivity was observed for Aβ, NDP, COL6A2, APOE and APCS in the white matter of control tissue (p-t). In the CADASIL case no Aβ pathology was present (u). Mild immunoreactivity for NDP (v) COL62A staining was most pronounced (w). APOE and APCS also displayed mild immunoreactivity related to the affected vessels (x, y). In hypertension related small vessel disease no Aβ was detected (z) immunoreactivity of COLA6A2 was moderate (ab) while immunoreactivity for NDP, APOE and APCS were low but present (aa, ac and ad). In the CARASAL case only prominent immunoreactivity of COL6A2 was seen in affected vessels (ag) while NDP, APOE and APCS were absent (af, ah and ai). Scale bar in (a) indicates 100 μm. ...View More

Datasheet

SDS

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Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

2C5-F2

Application

ELISA, WB

Immunogen

Full length recombinant COL6A2.

Host Species

Mouse

Specificity

Human

Antibody Isotype

IgG2a, κ

Clonality

Monoclonal Antibody

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS

Preservative

None

Concentration

Batch dependent

Purity

>95% as determined by analysis by SDS-PAGE

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

More Infomation

Target

Full Name

Collagen Type VI Alpha 2 Chain

Entrez Gene ID

1292

UniProt ID

P12110

Function

Collagen VI acts as a cell-binding protein.

Biological Process

Cell adhesion Source: UniProtKB-KW
Collagen fibril organization Source: Reactome
Extracellular matrix organization Source: Reactome
Response to glucose Source: Ensembl

Cellular Location

Extracellular matrix; Membrane. Recruited on membranes by CSPG4.

Involvement in disease

Bethlem myopathy 1 (BTHLM1):
A benign proximal myopathy characterized by early childhood onset and joint contractures most frequently affecting the elbows and ankles.
Ullrich congenital muscular dystrophy 1 (UCMD1):
A congenital myopathy characterized by muscle weakness and multiple joint contractures, generally noted at birth or early infancy. The clinical course is more severe than in Bethlem myopathy.
Myosclerosis autosomal recessive (MYOSAR):
A condition characterized by chronic inflammation of skeletal muscle with hyperplasia of the interstitial connective tissue. The clinical picture includes slender muscles with firm 'woody' consistency and restriction of movement of many joints because of muscle contractures.

PTM

Prolines at the third position of the tripeptide repeating unit (G-X-Y) are hydroxylated in some or all of the chains.

Tomassen, T., van de Ven, C., Anninga, J., Koelsche, C., Hiemcke-Jiwa, L. S., Ter Horst, S., ... & Flucke, U. (2021). Nodular fasciitis with malignant morphology and a COL6A2–USP6 fusion: a case report (of a 10-year-old boy). International Journal of Surgical Pathology, 29(6), 642-647.

Piao, X. M., Hwang, B., Jeong, P., Byun, Y. J., Kang, H. W., Seo, S. P., ... & Kim, W. J. (2021). Collagen type VI‑α1 and 2 repress the proliferation, migration and invasion of bladder cancer cells. International journal of oncology, 59(1), 1-13.

Liu, J., Wang, S., Hou, J., Cai, H., Pan, W., Dong, H., ... & Yu, B. (2020). Proteomics profiling reveals insulin-like growth factor 1, collagen type vi α-2 chain, and fermitin family homolog 3 as potential biomarkers of plaque erosion in st-segment elevated myocardial infarction. Circulation Journal, CJ-19.

Pham, H. T., Kram, V., Dar, Q. A., Komori, T., Ji, Y., Mohassel, P., ... & Young, M. F. (2020). Collagen VIα2 chain deficiency causes trabecular bone loss by potentially promoting osteoclast differentiation through enhanced TNFα signaling. Scientific reports, 10(1), 1-14.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

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For research use only. Not intended for any clinical use.

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