Mouse Anti-COP1 Recombinant Antibody (CBLC171-LY) (V2LY-1206-LY751)

Name: Mouse Anti-COP1 Recombinant Antibody (CBLC171-LY)
SKU: V2LY-1206-LY751
Rating: 5 (1 reviews)

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Published Data

Mouse Anti-COP1 Recombinant Antibody (CBLC171-LY) (V2LY-1206-LY751) in IHC

Representative DAB-stained skin sections at 06:00. ...View More

Németh, V., Horváth, S., Kinyó, Á., Gyulai, R., & Lengyel, Z. (2021). Expression patterns of clock gene mRNAs and clock proteins in human psoriatic skin samples. International Journal of Molecular Sciences, 23(1), 121.

Distributed under Open Access license CC BY 4.0, without modification.

Mouse Anti-COP1 Recombinant Antibody (CBLC171-LY) (V2LY-1206-LY751) in Immunoblot

Cells were transfected with indicated constructs and siRNA. Flag-Sox2 was immunoprecipated with anti-Flag and immunoblotted with anti-Myc. ...View More

Cui, C. P., Zhang, Y., Wang, C., Yuan, F., Li, H., Yao, Y., ... & Zhang, L. (2018). Dynamic ubiquitylation of Sox2 regulates proteostasis and governs neural progenitor cell differentiation. Nature communications, 9(1), 4648.

Distributed under Open Access license CC BY 4.0, without modification.

Mouse Anti-COP1 Recombinant Antibody (CBLC171-LY) (V2LY-1206-LY751) in IF

NPCs were plated in Matrigel-coated six-well plate for neuronal differentiation. For the indicated times, fixed and stained with anti-Sox2 (green), anti-COP1 (red), anti-TUJ1 (red), or CUL4A (green)-specific antibodies. Nuclei were counterstained with Hoechst 33342 (HO, blue). Scale bar, 50 µm. ...View More

Distributed under Open Access license CC BY 4.0, without modification.

Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

CBLC171-LY

Application

WB, IP, IF, IHC-P, ELISA

Immunogen

Amino acids 432-731 of human COP1.

Host Species

Mouse

Specificity

Mouse, Rat, Human

Antibody Isotype

IgG1, κ

Clonality

Monoclonal Antibody

Application Notes

Application	Note
WB	1:100-1:1,000
IP	1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)
IF(ICC)	1:50-1:500
ELISA	1:100-1:1,000
IHC-P	1:50-1:500

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

0.1% gelatin

Preservative

0.09% sodium azide

Concentration

0.2 mg/ml

Purity

>95% as determined by analysis by SDS-PAGE

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

More Infomation

Target

Full Name

COP1, E3 Ubiquitin Ligase

Entrez Gene ID

Human	64326
Mouse	26374
Rat	360860

UniProt ID

Human	Q8NHY2
Mouse	Q9R1A8
Rat	Q9JHC6

Function

E3 ubiquitin-protein ligase that mediates ubiquitination and subsequent proteasomal degradation of target proteins. E3 ubiquitin ligases accept ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. Involved in JUN ubiquitination and degradation. Directly involved in p53 (TP53) ubiquitination and degradation, thereby abolishing p53-dependent transcription and apoptosis. Ubiquitinates p53 independently of MDM2 or RCHY1. Probably mediates E3 ubiquitin ligase activity by functioning as the essential RING domain subunit of larger E3 complexes. In contrast, it does not constitute the catalytic RING subunit in the DCX DET1-COP1 complex that negatively regulates JUN, the ubiquitin ligase activity being mediated by RBX1. Involved in 14-3-3 protein sigma/SFN ubiquitination and proteasomal degradation, leading to AKT activation and promotion of cell survival. Ubiquitinates MTA1 leading to its proteasomal degradation. Upon binding to TRIB1, ubiquitinates CEBPA, which lacks a canonical COP1-binding motif (Probable).

Biological Process

Positive regulation of proteasomal ubiquitin-dependent protein catabolic process Source: UniProtKB
Post-translational protein modification Source: Reactome
Proteasome-mediated ubiquitin-dependent protein catabolic process Source: UniProtKB
Response to ionizing radiation Source: UniProtKB

Cellular Location

Cytoplasm; Nucleus speckle. In the nucleus, it forms nuclear speckles.

PTM

Autoubiquitinated. MTA1 destabilizes it by promoting its autoubiquitination.

Ponnu, J., & Hoecker, U. (2021). Illuminating the COP1/SPA ubiquitin ligase: fresh insights into its structure and functions during plant photomorphogenesis. Frontiers in Plant Science, 12, 486.

Zahid, S., Basharat, S., Fakhar, M., & Rashid, S. (2021). Molecular dynamics and structural analysis of the binding of COP1 E3 ubiquitin ligase to β‐catenin and TRIB pseudokinases. Proteins: Structure, Function, and Bioinformatics.

Ponnu, J. (2020). Molecular mechanisms suppressing COP1/SPA E3 ubiquitin ligase activity in blue light. Physiologia Plantarum, 169(3), 418-429.

Xu, D. (2020). COP1 and BBXs‐HY5‐mediated light signal transduction in plants. New Phytologist, 228(6), 1748-1753.

Blanco-Touriñán, N., Legris, M., Minguet, E. G., Costigliolo-Rojas, C., Nohales, M. A., Iniesto, E., ... & Alabadí, D. (2020). COP1 destabilizes DELLA proteins in Arabidopsis. Proceedings of the National Academy of Sciences, 117(24), 13792-13799.

Newton, K., Dugger, D. L., Sengupta-Ghosh, A., Ferrando, R. E., Chu, F., Tao, J., ... & Dixit, V. M. (2018). Ubiquitin ligase COP1 coordinates transcriptional programs that control cell type specification in the developing mouse brain. Proceedings of the National Academy of Sciences, 115(44), 11244-11249.

Podolec, R., & Ulm, R. (2018). Photoreceptor-mediated regulation of the COP1/SPA E3 ubiquitin ligase. Current Opinion in Plant Biology, 45, 18-25.

Durzynska, I., Xu, X., Adelmant, G., Ficarro, S. B., Marto, J. A., Sliz, P., ... & Blacklow, S. C. (2017). STK40 is a pseudokinase that binds the E3 ubiquitin ligase COP1. Structure, 25(2), 287-294.

Hoecker, U. (2017). The activities of the E3 ubiquitin ligase COP1/SPA, a key repressor in light signaling. Current opinion in plant biology, 37, 63-69.

Balcerowicz, M., Kerner, K., Schenkel, C., & Hoecker, U. (2017). SPA proteins affect the subcellular localization of COP1 in the COP1/SPA ubiquitin ligase complex during photomorphogenesis. Plant Physiology, 174(3), 1314-1321.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
Other Protocols

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For research use only. Not intended for any clinical use.

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