Rabbit Anti-COPS5 Recombinant Antibody (CBWJC-001) (V2LY-1206-LY770)

Name: Rabbit Anti-COPS5 Recombinant Antibody (CBWJC-001)
SKU: V2LY-1206-LY770
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Rabbit

Clone

CBWJC-001

Application

IHC, IP, WB, IF

Immunogen

Recombinant fusion protein corresponding to amino acids 1-100 of human COPS5.

Host Species

Rabbit

Specificity

Human, Mouse, Rat

Antibody Isotype

IgG

Clonality

Monoclonal Antibody

Application Notes

Application	Note
WB	1:500-1:2,000
IHC-P	1:50-1:200
IF(ICC)	1:50-1:200
IP	1:50-1:200

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS, pH 7.3, 50% glycerol, 0.05% BSA

Preservative

0.02% sodium azide

Concentration

1 mg/ml

Purity

>95% as determined by analysis by SDS-PAGE

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

More Infomation

Target

Full Name

COP9 Signalosome Subunit 5

Entrez Gene ID

Human	10987
Mouse	26754
Rat	312916

UniProt ID

Human	Q92905
Mouse	O35864
Rat	F7EUU4

Function

Probable protease subunit of the COP9 signalosome complex (CSN), a complex involved in various cellular and developmental processes. The CSN complex is an essential regulator of the ubiquitin (Ubl) conjugation pathway by mediating the deneddylation of the cullin subunits of the SCF-type E3 ligase complexes, leading to decrease the Ubl ligase activity of SCF-type complexes such as SCF, CSA or DDB2. The complex is also involved in phosphorylation of p53/TP53, c-jun/JUN, IkappaBalpha/NFKBIA, ITPK1 and IRF8, possibly via its association with CK2 and PKD kinases. CSN-dependent phosphorylation of TP53 and JUN promotes and protects degradation by the Ubl system, respectively. In the complex, it probably acts as the catalytic center that mediates the cleavage of Nedd8 from cullins. It however has no metalloprotease activity by itself and requires the other subunits of the CSN complex. Interacts directly with a large number of proteins that are regulated by the CSN complex, confirming a key role in the complex. Promotes the proteasomal degradation of BRSK2.

Biological Process

Exosomal secretion Source: FlyBase
Negative regulation of apoptotic process Source: UniProtKB
Nucleotide-excision repair, DNA damage recognition Source: Reactome
Positive regulation of DNA-binding transcription factor activity Source: UniProtKB
Positive regulation of transcription by RNA polymerase II Source: UniProtKB
Post-translational protein modification Source: Reactome
Protein deneddylation Source: UniProtKB
Protein deubiquitination Source: FlyBase
Regulation of cell cycle Source: Ensembl
Regulation of IRE1-mediated unfolded protein response Source: ParkinsonsUK-UCL
Regulation of JNK cascade Source: UniProtKB
Transcription-coupled nucleotide-excision repair Source: Reactome
Translation Source: ProtInc

Cellular Location

Cytosol; Nucleus; Perinuclear region; Synaptic vesicle. Nuclear localization is diminished in the presence of IFIT3.

Huang, Q., Liu, H., Zeng, J., Li, W., Zhang, S., Zhang, L., ... & Zhang, Z. (2020). COP9 signalosome complex subunit 5, an IFT20 binding partner, is essential to maintain male germ cell survival and acrosome biogenesis. Biology of reproduction, 102(1), 233-247.

Watanabe, K., Yokoyama, S., Kaneto, N., Hori, T., Iwakami, Y., Kato, S., ... & Saiki, I. (2018). COP9 signalosome subunit 5 regulates cancer metastasis by deubiquitinating SNAIL. Oncotarget, 9(29), 20670.

Zhou, R., Shao, Z., Liu, J., Zhan, W., Gao, Q., Pan, Z., ... & Zhao, L. (2018). COPS5 and LASP1 synergistically interact to downregulate 14‐3‐3σ expression and promote colorectal cancer progression via activating PI3K/AKT pathway. International Journal of Cancer, 142(9), 1853-1864.

Zhang, H., Zhong, A., Sun, J., Chen, M., Xie, S., Zheng, H., ... & Lu, R. (2017). COPS5 inhibition arrests the proliferation and growth of serous ovarian cancer cells via the elevation of p27 level. Biochemical and biophysical research communications, 493(1), 85-93.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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