Mouse Anti-CPLX4 Recombinant Antibody (171H8) (V2LY-1206-LY960)

Name: Mouse Anti-CPLX4 Recombinant Antibody (171H8)
SKU: V2LY-1206-LY960
Rating: 5 (1 reviews)

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Published Data

Mouse Anti-CPLX4 Recombinant Antibody (171H8) (V2LY-1206-LY960) in PLA

Spatial proximity of Cplx4 and Gt in light at rod photoreceptor ribbon synapses in vivo. Fluorescence micrographs of in situ proximity ligation assays (PLA) performed on vertical cryostat sections with antibodies against Gαt1 and Cplx4 (A,B) and Gγ1 and Cplx4 (A',B') after dark adaptation (A,A') and light adaptation (B,B'). ...View More

Lux, U. T., Meyer, J., Jahn, O., Davison, A., Babai, N., Gieβl, A., ... & Brandstätter, J. H. (2024). Light-dependent regulation of neurotransmitter release from rod photoreceptor ribbon synapses involves an interplay of Complexin 4 and Transducin with the SNARE complex. Frontiers in Molecular Neuroscience, 17, 1308466.

Distributed under Open Access license CC BY 4.0, without modification.

Mouse Anti-CPLX4 Recombinant Antibody (171H8) (V2LY-1206-LY960) in PLA

The spatial association of Gt and the SNARE complex at rod ribbon synapses is independent of Cplx4. Fluorescence micrographs of in situ proximity ligation assays (PLA) performed on vertical cryostat sections of Cplx4 WT (A,B) and Cplx4 KO mice (C,D) with antibodies against Gαt1 (magenta) and VAMP2 (green) after dark adaptation (A,C) and light adaptation (B,D). ...View More

Distributed under Open Access license CC BY 4.0, without modification.

Datasheet

SDS

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Basic Information

Host Animal

Mouse

Clone

171H8

Application

IHC, WB, IF, IP

Immunogen

Recombinant protein corresponding to aa1-160 from mouse Complexin4.

Host Species

Mouse

Specificity

Rat, Mouse

Antibody Isotype

IgG1, κ

Clonality

Monoclonal Antibody

Application Notes

Application	Note
WB	1:1,000
IHC	1:1,000-1:2,000

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Lyophilized

Buffer

PBS, Trehalose

Preservative

None

Purity

>95% as determined by analysis by SDS-PAGE

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

More Infomation

Target

Full Name

Complexin 4

Entrez Gene ID

Mouse	225644
Rat	361342

UniProt ID

Mouse	Q80WM3
Rat	D3ZM85

Function

Complexin that regulates SNARE protein complex-mediated synaptic vesicle fusion (By similarity).

Required for the maintenance of synaptic ultrastructure in the adult retina (By similarity).

Positively regulates synaptic transmission through synaptic vesicle availability and exocytosis of neurotransmitters at photoreceptor ribbon synapses in the retina (By similarity).

Suppresses tonic photoreceptor activity and baseline 'noise' by suppression of Ca2+ vesicle tonic release and the facilitation of evoked synchronous and asynchronous Ca2+ vesicle release (By similarity).

Biological Process

Regulation of neurotransmitter secretion Source: GO_Central
Response to stimulus Source: UniProtKB-KW
Synaptic vesicle exocytosis Source: GO_Central
Visual perception Source: UniProtKB-KW

Cellular Location

Cell membrane; Synapse. Enriched at the synaptic terminal.

PTM

Farnesylation mediates presynaptic targeting and is important for function in neurotransmitter release.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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