Rat Anti-CRB3 Recombinant Antibody (CBXC-2420) (CBMAB-C5700-CQ)

Name: Rat Anti-CRB3 Recombinant Antibody (CBXC-2420)
SKU: CBMAB-C5700-CQ
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Rat

Clone

CBXC-2420

Application

WB, IF, IHC

Immunogen

Full length protein.

Host Species

Rat

Specificity

Human, Mouse

Antibody Isotype

IgG2c

Clonality

Monoclonal Antibody

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Application	Note
WB	1:1,000
IF(ICC)	1:100
IHC	1:100

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Lyophilized

Buffer

PBS

Preservative

None

Concentration

LYOPH

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

More Infomation

Target

Full Name

Crumbs 3, Cell Polarity Complex Component

Introduction

CRB3 (Crumbs 3, Cell Polarity Complex Component) is a Protein Coding gene. Diseases associated with CRB3 include Leber Congenital Amaurosis 8 and Leber Congenital Amaurosis. Among its related pathways are Notch Signaling Pathway (sino) and PI3K-Akt signaling pathway. Gene Ontology (GO) annotations related to this gene include protein domain specific binding and SH3 domain binding.

Entrez Gene ID

Human	92359
Mouse	224912

UniProt ID

Human	Q9BUF7
Mouse	Q8QZT4

Alternative Names

Crumbs 3, Cell Polarity Complex Component; Crumbs Homolog 3 (Drosophila); Crumbs Family Member 3;

Function

Involved in the establishment of cell polarity in mammalian epithelial cells (PubMed:12771187, PubMed:14718572).

Regulates the morphogenesis of tight junctions (PubMed:12771187, PubMed:14718572).

Involved in promoting phosphorylation and cytoplasmic retention of transcriptional coactivators YAP1 and WWTR1/TAZ which leads to suppression of TGFB1-dependent transcription of target genes such as CCN2/CTGF, SERPINE1/PAI1, SNAI1/SNAIL1 and SMAD7 (By similarity).

Biological Process

Bicellular tight junction assembly Source: Reactome
Positive regulation of cell junction assembly Source: UniProtKB
Protein localization to plasma membrane Source: BHF-UCL

Cellular Location

Apical cell membrane; Tight junction. Localizes primarily to the apical membrane with a small fraction in the upper part of tight junctions of epithelial cells.

Topology

Extracellular: 27-59
Helical: 60-80
Cytoplasmic: 81-120

Pan, J., Liu, F., Xiao, X., Xu, R., Dai, L., Zhu, M., ... & Ji, G. (2022). METTL3 promotes colorectal carcinoma progression by regulating the m6A–CRB3–Hippo axis. Journal of Experimental & Clinical Cancer Research, 41(1), 1-17.

Khalilian, S., Hojati, Z., Dehghanian, F., Shaygannejad, V., Imani, S. Z. H., Kheirollahi, M., ... & Mirmosayyeb, O. (2021). Gene expression profiles of YAP1, TAZ, CRB3, and VDR in familial and sporadic multiple sclerosis among an Iranian population. Scientific reports, 11(1), 1-10.

Li, P., Zhou, C., Yan, Y., Li, J., Liu, J., Zhang, Y., & Liu, P. (2019). Crumbs protein homolog 3 (CRB3) expression is associated with oestrogen and progesterone receptor positivity in breast cancer. Clinical and Experimental Pharmacology and Physiology, 46(9), 837-844.

Madigan, V. J., Tyson, T. O., Yuziuk, J. A., Pillai, M., Moller-Tank, S., & Asokan, A. (2019). A CRISPR screen identifies the cell polarity determinant crumbs 3 as an adeno-associated virus restriction factor in hepatocytes. Journal of virology, 93(21), e00943-19.

Li, P., Liu, J., Li, J., & Liu, P. (2019). DNA methylation of CRB3 is a prognostic biomarker in clear cell renal cell carcinoma. Molecular biology reports, 46(4), 4377-4383.

Mao, X., Li, P., Wang, Y., Liang, Z., Liu, J., Li, J., ... & Liu, P. (2018). CRB3 regulates contact inhibition by activating the Hippo pathway in mammary epithelial cells. Cell death & disease, 8(1), e2546-e2546.

Li, P., Feng, C., Chen, H., Jiang, Y., Cao, F., Liu, J., & Liu, P. (2018). Elevated CRB 3 expression suppresses breast cancer stemness by inhibiting β‐catenin signalling to restore tamoxifen sensitivity. Journal of cellular and molecular medicine, 22(7), 3423-3433.

Walker, S. J., Selfors, L. M., Margolis, B. L., & Brugge, J. S. (2018). CRB3 and the FERM protein EPB41L4B regulate proliferation of mammary epithelial cells through the release of amphiregulin. Plos one, 13(11), e0207470.

Li, P., Wang, Y., Mao, X., Jiang, Y., Liu, J., Li, J., ... & Liu, P. (2017). CRB3 downregulation confers breast cancer stem cell traits through TAZ/β-catenin. Oncogenesis, 6(4), e322-e322.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
Other Protocols

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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