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Mouse Anti-ATF6B Recombinant Antibody (4D10) (CBMAB-A1848-LY)

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Datasheet
SDS
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Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal
Mouse
Clone
4D10
Application
WB, ELISA
Immunogen
CREBL1 (NP_004372.3, 2 a.a. ~ 88 a.a) partial recombinant protein with GST tag.
Specificity
Human
Antibody Isotype
IgG2a, κ
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
PBS, pH 7.4
Preservative
None
Concentration
Batch dependent
Purity
> 95% Purity determined by SDS-PAGE.
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.
More Infomation

Target

Full Name
Activating Transcription Factor 6 Beta
Introduction
The protein encoded by this gene is a transcription factor in the unfolded protein response (UPR) pathway during ER stress. Either as a homodimer or as a heterodimer with ATF6-alpha, the encoded protein binds to the ER stress response element, interacting with nuclear transcription factor Y to activate UPR target genes. The protein is normally found in the membrane of the endoplasmic reticulum; however, under ER stress, the N-terminal cytoplasmic domain is cleaved from the rest of the protein and translocates to the nucleus. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]
Entrez Gene ID
1388
UniProt ID
Q99941
Alternative Names
CREB-RP; CREBL1; FLJ10066; G13
Function
Cyclic AMP-dependent transcription factor ATF-6 beta: Precursor of the transcription factor form (Processed cyclic AMP-dependent transcription factor ATF-6 beta), which is embedded in the endoplasmic reticulum membrane (PubMed:11256944).
Endoplasmic reticulum stress promotes processing of this form, releasing the transcription factor form that translocates into the nucleus, where it activates transcription of genes involved in the unfolded protein response (UPR) (PubMed:11256944).
Processed cyclic AMP-dependent transcription factor ATF-6 beta: Transcription factor that acts in the unfolded protein response (UPR) pathway by activating UPR target genes induced during ER stress (PubMed:11256944).
Binds DNA on the 5'-CCAC[GA]-3' half of the ER stress response element (ERSE) (5'-CCAATN9CCAC[GA]-3') when NF-Y is bound to ERSE (PubMed:11256944).
Biological Process
ATF6-mediated unfolded protein response Source: ParkinsonsUK-UCL
Endoplasmic reticulum unfolded protein response Source: GO_Central
Negative regulation of ATF6-mediated unfolded protein response Source: UniProtKB
Positive regulation of transcription by RNA polymerase II Source: NTNU_SB
Positive regulation of transcription from RNA polymerase II promoter in response to endoplasmic reticulum stress Source: ParkinsonsUK-UCL
Regulation of transcription by RNA polymerase II Source: GO_Central
Signal transduction Source: ProtInc
Cellular Location
Endoplasmic reticulum membrane
Processed cyclic AMP-dependent transcription factor ATF-6 beta: Nucleus. Under ER stress the cleaved N-terminal cytoplasmic domain translocates into the nucleus.
Topology
Cytoplasmic: 2-396 aa
Helical: 397-417 aa
Lumenal: 418-703 aa
PTM
N-glycosylated.
During unfolded protein response, a fragment of approximately 60 kDa containing the cytoplasmic transcription factor domain is released by proteolysis. The cleavage is probably performed sequentially by site-1 (MBTPS1, S1P) and site-2 (MBTPS2, S2P) proteases.

Moudi, M., Sargazi, S., Heidari Nia, M., Saravani, R., Shirvaliloo, M., & Shakiba, M. (2020). Polymorphism in the 3′-UTR of LIF but not in the ATF6B gene associates with schizophrenia susceptibility: a case-control study and in silico analyses. Journal of Molecular Neuroscience, 70, 2093-2101.

Correll, R. N., Grimes, K. M., Prasad, V., Lynch, J. M., Khalil, H., & Molkentin, J. D. (2019). Overlapping and differential functions of ATF6α versus ATF6β in the mouse heart. Scientific reports, 9(1), 1-8.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

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