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Rabbit Anti-CRHR1 Recombinant Antibody (EG829) (V2LY-1206-LY1158)


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Summary

Host Animal
Rabbit
Specificity
Human, Rat
Clone
EG829
Antibody Isotype
IgG
Application
IF, WB, IHC

Basic Information

Immunogen
Peptide corresponding to human CRHR1 (aa425-444).
Host Species
Rabbit
Specificity
Human, Rat
Antibody Isotype
IgG
Clonality
Monoclonal Antibody
Application Notes
ApplicationNote
WB1:500
IF(ICC)1:500
IHC1:500

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
PBS, pH 7.4
Preservative
0.09% sodium azide
Concentration
0.5 mg/ml
Purity
>95% as determined by analysis by SDS-PAGE
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Target

Full Name
Corticotropin Releasing Hormone Receptor 1
Entrez Gene ID
Human1394
Rat58959
UniProt ID
HumanP34998
RatP35353
Function
G-protein coupled receptor for CRH (corticotropin-releasing factor) and UCN (urocortin). Has high affinity for CRH and UCN. Ligand binding causes a conformation change that triggers signaling via guanine nucleotide-binding proteins (G proteins) and down-stream effectors, such as adenylate cyclase. Promotes the activation of adenylate cyclase, leading to increased intracellular cAMP levels. Inhibits the activity of the calcium channel CACNA1H. Required for normal embryonic development of the adrenal gland and for normal hormonal responses to stress. Plays a role in the response to anxiogenic stimuli.
Biological Process
Activation of adenylate cyclase activity Source: ProtInc
Adenylate cyclase-activating G protein-coupled receptor signaling pathway Source: UniProtKB
Adenylate cyclase-modulating G protein-coupled receptor signaling pathway Source: GO_Central
Cell surface receptor signaling pathway Source: InterPro
Cellular response to corticotropin-releasing hormone stimulus Source: UniProtKB
Corticotropin secretion Source: UniProtKB
Female pregnancy Source: ProtInc
G protein-coupled receptor signaling pathway Source: Reactome
Immune response Source: ProtInc
Negative regulation of inflammatory response to antigenic stimulus Source: Reactome
Negative regulation of voltage-gated calcium channel activity Source: UniProtKB
Parturition Source: ProtInc
Regulation of adenylate cyclase activity involved in G protein-coupled receptor signaling pathway Source: UniProtKB
Regulation of corticosterone secretion Source: UniProtKB
Cellular Location
Endosome; Cell membrane. Agonist-binding promotes endocytosis.
Topology
Extracellular: 24-111
Helical: 112-142
Cytoplasmic: 143-178
Helical: 179-203
Extracellular: 204-218
Helical: 219-247
Cytoplasmic: 248-254
Helical: 255-282
Extracellular: 283-298
Helical: 299-324
Cytoplasmic: 325-335
Helical: 336-360
Extracellular: 361-367
Helical: 368-397
Cytoplasmic: 398-444
PTM
C-terminal Ser or Thr residues may be phosphorylated.
Phosphorylation at Ser-330 by PKA prevents maximal coupling to Gq-protein, and thereby negatively regulates downstream signaling.
More Infomation

Ramoz, N., Hoertel, N., Nobile, B., Voegeli, G., Nasr, A., Le Strat, Y., ... & Gorwood, P. (2020). Corticotropin releasing hormone receptor CRHR1 gene is associated with tianeptine antidepressant response in a large sample of outpatients from real-life settings. Translational psychiatry, 10(1), 1-12.

Kameda, H., Yamamoto, M., Tone, Y., Tone, M., & Melmed, S. (2019). Proton sensitivity of corticotropin-releasing hormone receptor 1 signaling to proopiomelanocortin in male mice. Endocrinology, 160(2), 276-291.

He, S. C., Wu, S., Du, X. D., Jia, Q., Wang, C., Wu, F., ... & Zhang, X. Y. (2019). Interactive effects of corticotropin-releasing hormone receptor 1 gene and work stress on burnout in medical professionals in a Chinese Han population. Journal of affective disorders, 252, 1-8.

Jhang, J. F., Birder, L. A., Jiang, Y. H., Hsu, Y. H., Ho, H. C., & Kuo, H. C. (2019). Dysregulation of bladder corticotropin-releasing hormone receptor in the pathogenesis of human interstitial cystitis/bladder pain syndrome. Scientific reports, 9(1), 1-8.

Park, J., Sung, J. Y., Kim, D. K., Kong, I. D., Hughes, T. L., & Kim, N. (2018). Genetic association of human Corticotropin-Releasing Hormone Receptor 1 (CRHR1) with Internet gaming addiction in Korean male adolescents. BMC psychiatry, 18(1), 1-10.

Torres-Reverón, A., Rivera-Lopez, L. L., Flores, I., & Appleyard, C. B. (2018). Antagonizing the corticotropin releasing hormone receptor 1 with antalarmin reduces the progression of endometriosis. PLoS One, 13(11), e0197698.

Li, B., Lee, C., Filler, T., Hock, A., Wu, R. Y., Li, Q., ... & Pierro, A. (2017). Inhibition of corticotropin-releasing hormone receptor 1 and activation of receptor 2 protect against colonic injury and promote epithelium repair. Scientific reports, 7(1), 1-11.

Grimm, S., Wirth, K., Fan, Y., Weigand, A., Gärtner, M., Feeser, M., ... & Aust, S. (2017). The interaction of corticotropin-releasing hormone receptor gene and early life stress on emotional empathy. Behavioural Brain Research, 329, 180-185.

Bernardi, R. E., Broccoli, L., Hirth, N., Justice, N. J., Deussing, J. M., Hansson, A. C., & Spanagel, R. (2017). Dissociable role of corticotropin releasing hormone receptor subtype 1 on dopaminergic and D1 dopaminoceptive neurons in cocaine seeking behavior. Frontiers in behavioral neuroscience, 11, 221.

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For research use only. Not intended for any clinical use.

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