Mouse Anti-CSK Recombinant Antibody (CBXC-3040) (V2LY-1206-LY1504)

Name: Mouse Anti-CSK Recombinant Antibody (CBXC-3040)
SKU: V2LY-1206-LY1504
Rating: 5 (1 reviews)

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Datasheet

SDS

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Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

CBXC-3040

Application

WB, IF

Immunogen

CSK Fusion Protein Ag12103 (83-450 aa).

Host Species

Mouse

Specificity

Human, Mouse

Antibody Isotype

IgG2b

Clonality

Monoclonal Antibody

Application Notes

Application	Note
WB	1:500-1:2,000
IF(ICC)	1:20-1:200

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS, pH 7.3, 50% glycerol

Preservative

0.02% sodium azide

Concentration

Batch dependent

Purity

>95% as determined by analysis by SDS-PAGE

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

More Infomation

Target

Full Name

C-Terminal Src Kinase

Entrez Gene ID

Human	1445
Mouse	12988

UniProt ID

Human	P41240
Mouse	P41241

Function

Non-receptor tyrosine-protein kinase that plays an important role in the regulation of cell growth, differentiation, migration and immune response. Phosphorylates tyrosine residues located in the C-terminal tails of Src-family kinases (SFKs) including LCK, SRC, HCK, FYN, LYN, CSK or YES1. Upon tail phosphorylation, Src-family members engage in intramolecular interactions between the phosphotyrosine tail and the SH2 domain that result in an inactive conformation. To inhibit SFKs, CSK is recruited to the plasma membrane via binding to transmembrane proteins or adapter proteins located near the plasma membrane. Suppresses signaling by various surface receptors, including T-cell receptor (TCR) and B-cell receptor (BCR) by phosphorylating and maintaining inactive several positive effectors such as FYN or LCK.

Biological Process

Adaptive immune response Source: UniProtKB-KW
Adherens junction organization Source: GO_Central
Brain development Source: Ensembl
Cellular response to peptide hormone stimulus Source: Ensembl
Cytokine-mediated signaling pathway Source: Reactome
Negative regulation of bone resorption Source: Ensembl
Negative regulation of cell population proliferation Source: Ensembl
Negative regulation of ERK1 and ERK2 cascade Source: Ensembl
Negative regulation of Golgi to plasma membrane protein transport Source: UniProtKB
Negative regulation of interleukin-6 production Source: Ensembl
Negative regulation of kinase activity Source: Ensembl
Negative regulation of low-density lipoprotein particle clearance Source: Ensembl
Negative regulation of phagocytosis Source: Ensembl
Oligodendrocyte differentiation Source: Ensembl
Positive regulation of MAP kinase activity Source: Ensembl
Protein autophosphorylation Source: Ensembl
Protein phosphorylation Source: ProtInc
Regulation of Fc receptor mediated stimulatory signaling pathway Source: GO_Central
T cell costimulation Source: Reactome
T cell receptor signaling pathway Source: Reactome

Cellular Location

Cell membrane; Cytoplasm. Mainly cytoplasmic, also present in lipid rafts.

PTM

Phosphorylated at Ser-364 by PKA, leading to increased activity. Autophosphorylated.

Xiao, L., Salem, J. E., Clauss, S., Hanley, A., Bapat, A., Hulsmans, M., ... & Milan, D. J. (2020). Ibrutinib-mediated atrial fibrillation attributable to inhibition of C-terminal Src kinase. Circulation, 142(25), 2443-2455.

P O’Malley, D. (2020). Recent advances in inhibitors of C-terminal SRC kinase. Future Medicinal Chemistry, 12(16), 1447-1449.

Simoncelli, S., Griffié, J., Williamson, D. J., Bibby, J., Bray, C., Zamoyska, R., ... & Owen, D. M. (2020). Multi-color molecular visualization of signaling proteins reveals how C-terminal Src kinase nanoclusters regulate T cell receptor activation. Cell Reports, 33(12), 108523.

O’Malley, D. P., Ahuja, V., Fink, B., Cao, C., Wang, C., Swanson, J., ... & Xie, D. (2019). Discovery of pyridazinone and pyrazolo [1, 5-a] pyridine inhibitors of C-terminal Src kinase. ACS medicinal chemistry letters, 10(10), 1486-1491.

Samarasekera, G. G., & Auld, V. J. (2018). C-terminal Src kinase (Csk) regulates the tricellular junction protein Gliotactin independent of Src. Molecular biology of the cell, 29(2), 123-136.

Potuckova, L., Draberova, L., Halova, I., Paulenda, T., & Draber, P. (2018). Positive and negative regulatory roles of C-terminal Src kinase (CSK) in FcεRI-mediated mast cell activation, independent of the transmembrane adaptor PAG/CSK-binding protein. Frontiers in immunology, 9, 1771.

Berman-Booty, L. D., Eraslan, R., Hanumegowda, U., Cantor, G. H., Bounous, D. I., Janovitz, E. B., ... & Wee, S. (2018). Systemic loss of C-terminal Src kinase expression elicits spontaneous suppurative inflammation in conditional knockout mice. Veterinary pathology, 55(2), 331-340.

Taskinen, B., Ferrada, E., & Fowler, D. M. (2017). Early emergence of negative regulation of the tyrosine kinase Src by the C-terminal Src kinase. Journal of Biological Chemistry, 292(45), 18518-18529.

Liu, D., Yuan, Y., Xu, R., & Cowburn, D. (2017). Domain interactions of C-terminal Src Kinase determined through NMR spectroscopy with segmental isotope labeling. Protein & cell, 8(1), 67-71.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

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