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Mouse Anti-CSTF2 Recombinant Antibody (CBXC-1796) (V2LY-0125-LY193)


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Published Data
V2LY-0125-LY193-1
Mouse Anti-CSTF2 Recombinant Antibody (CBXC-1796) (V2LY-0125-LY193) in WB
Western blot analysis of C/P factors CstF64, CstF64τ and macrophage-specific markers CD68, HLA-DR, CD38 and the monocyte marker CD14 in U937 cells stably transfected with control overexpression vector (OE-control) and those overexpressing CstF64 (OE-CstF64) and treated with 3 nM doxycycline for 3 days prior to harvesting. No PMA treatment has been done. ...View More
Mukherjee, S., Graber, J. H., & Moore, C. L. (2023). Macrophage differentiation is marked by increased abundance of the mRNA 3'end processing machinery, altered poly (A) site usage, and sensitivity to the level of CstF64. Frontiers in immunology, 14, 1091403. Distributed under Open Access license CC BY 4.0, without modification.
V2LY-0125-LY193-2
Mouse Anti-CSTF2 Recombinant Antibody (CBXC-1796) (V2LY-0125-LY193) in Immunoblotting
Total nuclear extracts from HEK293 cells co-transfected with vector or HuR were incubated with biotinylated RNA oligo corresponding to DSE. Immunoblot analysis of RNA-bound protein fractions probed with anti-CstF-64 antibody. ...View More
Gong, Q., Stump, M. R., & Zhou, Z. (2018). Regulation of Kv11. 1 potassium channel C-terminal isoform expression by the RNA-binding proteins HuR and HuD. Journal of Biological Chemistry, 293(51), 19624-19632. Distributed under Open Access license CC BY 4.0, without modification.

Summary

Host Animal
Mouse
Specificity
Human, Mouse, Rat
Clone
CBXC-1796
Antibody Isotype
IgM, κ
Application
WB, IP, IF, ELISA, IHC-P

Basic Information

Immunogen
Amino acids 278-577 near the C-terminus of human CstF-64.
Host Species
Mouse
Specificity
Human, Mouse, Rat
Antibody Isotype
IgM, κ
Clonality
Monoclonal Antibody
Application Notes
ApplicationNote
WB1:100-1:1,000
IP1-2 μg per 100-500 μg of total protein (1 mL of cell lysate)
IF(ICC)1:50-1:500
ELISA1:100-1:1,000
IHC-P1:50-1:500

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
Gelatin & PBS
Preservative
Sodium Azide
Concentration
0.2 mg/mL
Purity
>95% as determined by analysis by SDS-PAGE
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Target

Full Name
Cleavage Stimulation Factor Subunit 2
Entrez Gene ID
1478
UniProt ID
P33240
Function
One of the multiple factors required for polyadenylation and 3'-end cleavage of mammalian pre-mRNAs. This subunit is directly involved in the binding to pre-mRNAs (By similarity).
Biological Process
mRNA 3'-end processing Source: Reactome
mRNA polyadenylation Source: InterPro
mRNA splicing, via spliceosome Source: Reactome
Pre-mRNA cleavage required for polyadenylation Source: GO_Central
Termination of RNA polymerase II transcription Source: Reactome
tRNA splicing, via endonucleolytic cleavage and ligation Source: Reactome
Cellular Location
Nucleus. Localized with DDX1 in cleavage bodies.
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For research use only. Not intended for any clinical use.

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