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Mouse Anti-CTCFL Recombinant Antibody (2B12E2) (CBMAB-C4674-LY)

This product is antibody recognizes CTCFL. The antibody 2B12E2 immunoassay techniques such as: ELISA, WB, IF.
See all CTCFL antibodies

Summary

Host Animal
Mouse
Specificity
Human, Mouse
Clone
2B12E2
Antibody Isotype
IgG1
Application
ELISA, WB, IF

Basic Information

Specificity
Human, Mouse
Antibody Isotype
IgG1
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Target

Full Name
CCCTC-Binding Factor Like
Introduction
CTCF (CCCTC-Binding Factor) is a Protein Coding gene. Diseases associated with CTCF include Mental Retardation, Autosomal Dominant 21 and Spherocytosis, Type 4. Among its related pathways are Activated PKN1 stimulates transcription of AR (androgen receptor) regulated genes KLK2 and KLK3 and Sudden Infant Death Syndrome (SIDS) Susceptibility Pathways.
Gene Ontology (GO) annotations related to this gene include DNA binding transcription factor activity and chromatin binding.
An important paralog of this gene is CTCFL.
Entrez Gene ID
Human140690
Mouse664799
UniProt ID
HumanQ8NI51
MouseA2APF3
Function
Testis-specific DNA binding protein responsible for insulator function, nuclear architecture and transcriptional control, which probably acts by recruiting epigenetic chromatin modifiers. Plays a key role in gene imprinting in male germline, by participating in the establishment of differential methylation at the IGF2/H19 imprinted control region (ICR). Directly binds the unmethylated H19 ICR and recruits the PRMT7 methyltransferase, leading to methylate histone H4 'Arg-3' to form H4R3sme2. This probably leads to recruit de novo DNA methyltransferases at these sites (By similarity).

Seems to act as tumor suppressor. In association with DNMT1 and DNMT3B, involved in activation of BAG1 gene expression by binding to its promoter. Required for dimethylation of H3 lysine 4 (H3K4me2) of MYC and BRCA1 promoters.
Biological Process
Cell cycle Source: UniProtKB-KW
DNA methylation involved in gamete generation Source: UniProtKB
Histone methylation Source: UniProtKB
Positive regulation of gene expression Source: UniProtKB
Positive regulation of transcription, DNA-templated Source: UniProtKB
Positive regulation of transcription by RNA polymerase II Source: NTNU_SB
Regulation of gene expression by genetic imprinting Source: UniProtKB
Regulation of histone H3-K4 methylation Source: UniProtKB
Regulation of transcription by RNA polymerase II Source: GO_Central
Cellular Location
Nucleus; Cytoplasm

Rao, G. K., Makani, V. K. K., Mendonza, J. J., Edathara, P. M., Patel, N., Ramakrishna, M., ... & Bhadra, M. P. (2021). Downregulation of BORIS/CTCFL leads to ROS‐dependent cellular senescence and drug sensitivity in MYCN‐amplified neuroblastoma. The FEBS journal.

Moscona, R., Janssen, S., Elchebly, M., Papadakis, A., Spatz, A., & Rubin, E. (2022). ATAC-Seq and RNA-Seq combined analysis elucidates the impact of BORIS/CTCFL on melanoma progression, mediated by chromatin accessibility and gene expression alterations. bioRxiv.

Chen, M. M., Zhao, R. C., Chen, K. F., Huang, Y., Liu, Z. J., Wei, Y. G., ... & Qin, Y. (2020). Hypomethylation of CTCFL promoters as a noninvasive biomarker in plasma from patients with hepatocellular carcinoma. Neoplasma, 67(04), 909-15.

Janssen, S. M., Moscona, R., Elchebly, M., Papadakis, A. I., Redpath, M., Wang, H., ... & Spatz, A. (2020). BORIS/CTCFL promotes a switch from a proliferative towards an invasive phenotype in melanoma cells. Cell death discovery, 6(1), 1-17.

Debaugny, R. E., & Skok, J. A. (2020). CTCF and CTCFL in cancer. Current opinion in genetics & development, 61, 44-52.

Gong, M., Yan, C., Jiang, Y., Meng, H., Feng, M., & Cheng, W. (2019). Genome‑wide bioinformatics analysis reveals CTCFL is upregulated in high‑grade epithelial ovarian cancer. Oncology letters, 18(4), 4030-4039.

Voutsadakis, I. A. (2018). Molecular Lesions of Insulator CTCF and Its Paralogue CTCFL (BORIS) in cancer: an analysis from published genomic studies. High-throughput, 7(4), 30.

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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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