Mouse Anti-CUX2 Recombinant Antibody (2H8) (V2LY-0125-LY556)

Name: Mouse Anti-CUX2 Recombinant Antibody (2H8)
SKU: V2LY-0125-LY556
Rating: 5 (1 reviews)

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Published Data

Mouse Anti-CUX2 Recombinant Antibody (2H8) (V2LY-0125-LY556) in WB

RNA-seq gene expression data for mature immune cells from the Human Protein Atlas shows CUX2 expression exclusively in myeloid or plasmacytoid DCs (above). Gene expression profiling data for BPDCN and AML patients from dataset GSE shows CUX2 expression exclusively in BPDCN patients (middle). RQ-PCR and Western blot analyses show CUX2 expression in pDC cell line CAL-1 and primary DCs (below). ...View More

Nagel, S., Rand, U., Pommerenke, C., & Meyer, C. (2024). Transcriptional Landscape of CUT-Class Homeobox Genes in Blastic Plasmacytoid Dendritic Cell Neoplasm. International Journal of Molecular Sciences, 25(5), 2764. Distributed under Open Access license CC BY 4.0, without modification.

Mouse Anti-CUX2 Recombinant Antibody (2H8) (V2LY-0125-LY556) in IHC

CUX2 is expressed in MZ, deep CP and pSP during the pre-subplate phase at 10 PCW. (A) Complete scan of the parasagittal section through the telencephalon of the human fetus immunostained for CUX2. (B) Higher magnification light microscopy images of box denoted in A showing developing neocortical wall from the surface-placed Marginal Zone (MZ) to proliferative Ventricular Zone (VZ). (C) Parasagittal section through the telencephalon of the human fetus at 10 PCW was immunostained for CUX2. (C) CUX2+ nuclei were found in the MZ the deep Cortical Plate (CP; blue arrowhead) and pre-Subplate (pSP; yellow arrowhead), with strongly reactive nuclei in the MZ (green arrowhead). SVZ, Subventricular Zone; IZ, Intermediate Zone. Scale bar = 100 µm (A). ...View More

Miškić, T., Kostović, I., Rašin, M. R., & Krsnik, Ž. (2021). Adult upper cortical layer specific transcription factor CUX2 is expressed in transient subplate and marginal zone neurons of the developing human brain. Cells, 10(2), 415. Distributed under Open Access license CC BY 4.0, without modification.

Mouse Anti-CUX2 Recombinant Antibody (2H8) (V2LY-0125-LY556) in IF

CUX2 co-localizes with Cajal-Retzius neuron subtype marker Reelin in the MZ during the pSP phase at 12 PCW. (A,B) Midlateral portion of a prospective precentral region of the developing cerebral wall (indicated by box in the Nissl stained complete scan of the coronal section in the lower left corner) during the pSP phase was immunostained for CUX2 (green), Reelin (red), and DAPI (blue). (A) A representative confocal image shows evenly spaced CUX2+ nuclei were located in the MZ, while CUX2 was not detected in proliferative VZ and SVZ. (B) Reelin co-localized with CUX2+ nuclei in the upper third of the MZ (magnified in A' and B'). Scale bar = 50 µm. ...View More

Datasheet

SDS

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Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

2H8

Application

ELISA, WB

Immunogen

CUX2 (121-220aa) partial recombinant protein.

Host Species

Mouse

Specificity

Human

Antibody Isotype

IgG1, κ

Clonality

Monoclonal Antibody

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS

Preservative

None

Concentration

Batch dependent

Purity

>95% as determined by analysis by SDS-PAGE

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

More Infomation

Target

Full Name

cut-like homeobox 2

Entrez Gene ID

23316

UniProt ID

O14529

Function

Transcription factor involved in the control of neuronal proliferation and differentiation in the brain. Regulates dendrite development and branching, dendritic spine formation, and synaptogenesis in cortical layers II-III. Binds to DNA in a sequence-specific manner.

Biological Process

Cellular response to organic substance Source: Ensembl
Cognition Source: UniProtKB
Negative regulation of transcription by RNA polymerase II Source: UniProtKB
Positive regulation of dendrite morphogenesis Source: UniProtKB
Positive regulation of dendritic spine morphogenesis Source: UniProtKB
Positive regulation of excitatory postsynaptic potential Source: UniProtKB
Positive regulation of gene expression Source: UniProtKB
Positive regulation of synapse assembly Source: UniProtKB
Regulation of transcription by RNA polymerase II Source: GO_Central
Short-term memory Source: UniProtKB

Cellular Location

Nucleus

Involvement in disease

Developmental and epileptic encephalopathy 67 (DEE67):
A form of epileptic encephalopathy, a heterogeneous group of severe childhood onset epilepsies characterized by refractory seizures, neurodevelopmental impairment, and poor prognosis. Development is normal prior to seizure onset, after which cognitive and motor delays become apparent. DEE67 is an autosomal dominant form characterized by onset of seizures in infancy. Later onset of seizures in childhood may occur in some patients.

Miškić, T., Kostović, I., Rašin, M. R., & Krsnik, Ž. (2021). Adult Upper Cortical Layer Specific Transcription Factor CUX2 Is Expressed in Transient Subplate and Marginal Zone Neurons of the Developing Human Brain. Cells, 10(2), 415.

Chen, I., Kuo, P. H., Yang, A. C., Tsai, S. J., Liu, T. H., Liu, H. J., ... & Liu, Y. L. (2020). CUX2, BRAP and ALDH2 are associated with metabolic traits in people with excessive alcohol consumption. Scientific reports, 10(1), 1-11.

Yang, H., Kim, J., Kim, Y., Jang, S. W., Sestan, N., & Shim, S. (2020). Cux2 expression regulated by Lhx2 in the upper layer neurons of the developing cortex. Biochemical and Biophysical Research Communications, 521(4), 874-879.

Pan, H., Yang, M., Lu, L., Tao, B., He, X., Chen, H., ... & Tang, S. (2019). Association of FAM65B, AGBL4, and CUX2 genetic polymorphisms with susceptibility to antituberculosis drug-induced hepatotoxicity: validation study in a Chinese Han population. Pharmacogenetics and genomics, 29(4), 84-90.

Sun, Y., Ye, D., Li, Y., Chen, E., Hao, R., Cai, Y., ... & Zhang, X. (2019). CUX2 functions as an oncogene in papillary thyroid cancer. OncoTargets and therapy, 12, 217.

Fregoso, S. P., Dwyer, B. E., & Franco, S. J. (2019). Lmx1a drives Cux2 expression in the cortical hem through activation of a conserved intronic enhancer. Development, 146(5), dev170068.

Chatron, N., Møller, R. S., Champaigne, N. L., Schneider, A. L., Kuechler, A., Labalme, A., ... & Lesca, G. (2018). The epilepsy phenotypic spectrum associated with a recurrent CUX2 variant. Annals of neurology, 83(5), 926-934.

Barington, M., Risom, L., Ek, J., Uldall, P., & Ostergaard, E. (2018). A recurrent de novo CUX2 missense variant associated with intellectual disability, seizures, and autism spectrum disorder. European Journal of Human Genetics, 26(9), 1388-1391.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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