Mouse Anti-CYB5R2 Recombinant Antibody (4B9) (V2LY-0125-LY836)

Name: Mouse Anti-CYB5R2 Recombinant Antibody (4B9)
SKU: V2LY-0125-LY836
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

4B9

Application

FC, WB

Immunogen

Full length human recombinant protein of human CYB5R2 produced in HEK293T cell.

Host Species

Mouse

Specificity

Human

Antibody Isotype

IgG2a

Clonality

Monoclonal Antibody

Application Notes

Application	Note
WB	1:2,000
FC	1:100

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

BSA, Glycerol & PBS

Preservative

Sodium Azide

Concentration

1 mg/mL

Purity

>95% as determined by analysis by SDS-PAGE

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

More Infomation

Target

Full Name

Cytochrome B5 Reductase 2

Entrez Gene ID

51700

UniProt ID

Q6BCY4

Function

NADH-cytochrome b5 reductases are involved in desaturation and elongation of fatty acids, cholesterol biosynthesis, drug metabolism, and, in erythrocyte, methemoglobin reduction (By similarity).

Responsible for NADH-dependent lucigenin chemiluminescence in spermatozoa by reducing both lucigenin and 2-[4-iodophenyl]-3-[4-nitrophenyl]-5-[2,4-disulfophenyl]-2H tetrazolium monosodium salt (WST-1).

Biological Process

Bicarbonate transport Source: Reactome
Sterol biosynthetic process Source: UniProtKB-KW

Cellular Location

Endoplasmic reticulum membrane; Nucleus; Membrane

Chen, J., Liao, L., Xu, H., Zhang, Z., & Zhang, J. (2021). Long non-coding RNA MEG3 inhibits neovascularization in diabetic retinopathy by regulating microRNA miR-6720-5p and cytochrome B5 reductase 2. Bioengineered, 12(2), 11872-11884.

Najafi, Z., Mohamadnia, A., Ahmadi, R., Mahmoodi, M., Bahrami, N., Khosravi, A., ... & Jarrahi, A. M. (2020). The Identification of Cytochrome B5 Reductase 2 and Fructose-1, 6-Bisphosphatase 1 Proteins in Non-Small Cell Lung Cancer: Proteomics Approach. International Journal of Cancer Management, 13(12).

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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