Mouse Anti-CYCS Recombinant Antibody (CBYY-C2386) (CBMAB-C3824-YY)

Name: Mouse Anti-CYCS Recombinant Antibody (CBYY-C2386)
SKU: CBMAB-C3824-YY
Rating: 5 (1 reviews)

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Published Data

Mouse Anti-CYCS Recombinant Antibody (CBYY-C2386) (CBMAB-C3824-YY) in WB

The cell lysates were analyzed by western blotting with anti-cytochrome c, anti-Cox IV (mitochondria marker), and anti-triosephosphate isomerase (TPI, cytosol marker). ...View More

Murata, N., Nishimura, K., Harada, N., Kitakaze, T., Yoshihara, E., Inui, H., & Yamaji, R. (2024). Insulin reduces endoplasmic reticulum stress-induced apoptosis by decreasing mitochondrial hyperpolarization and caspase-12 in INS-1 pancreatic β-cells. Physiological Reports, 12(12), e16106. Distributed under Open Access license CC BY 4.0, without modification.

Mouse Anti-CYCS Recombinant Antibody (CBYY-C2386) (CBMAB-C3824-YY) in WB

EV-classical markers CD9, CD81, CD63, Flotillin-1 and Alix are represented in different samples. THP (urine contaminant), Lamin A/C and Cytochrome C (associated with intracellular compartments) markers are also represented. PC3 cell line lysate was used
as a positive control for Lamin A/C and Cytochrome C markers. Western-blot images were cropped; the original blots are presented in Figure S10 from Supplementary Material 2. ...View More

Teixeira-Marques, A., Monteiro-Reis, S., Montezuma, D., Lourenço, C., Oliveira, M. C., Constâncio, V., ... & Jerónimo, C. (2024). Improved recovery of urinary small extracellular vesicles by differential ultracentrifugation. Scientific Reports, 14(1), 12267. Distributed under Open Access license CC BY 4.0, without modification.

Mouse Anti-CYCS Recombinant Antibody (CBYY-C2386) (CBMAB-C3824-YY) in WB

LoVo cells treated with HAs (3 mg/mL) for 24-48 h. Cells subjected to Western blotting to analyze signaling pathways related to mitochondria membrane potential. ...View More

Tsai, M. C., Chen, C. C., Tseng, T. H., Chang, Y. C., Lin, Y. J., Tsai, I. N., ... & Wang, C. J. (2023). Hibiscus anthocyanins extracts induce apoptosis by activating AMP-activated protein kinase in human colorectal cancer cells. Nutrients, 15(18), 3972. Distributed under Open Access license CC BY 4.0, without modification.

Mouse Anti-CYCS Recombinant Antibody (CBYY-C2386) (CBMAB-C3824-YY) in WB

Western blot analysis for proliferation or apoptosis markers. ...View More

Dena-Beltrán, J. L., Nava-Domínguez, P., Palmerín-Carreño, D., Martínez-Alarcón, D., Moreno-Celis, U., Valle-Pacheco, M., ... & García-Gasca, T. (2023). EGFR and p38MAPK Contribute to the Apoptotic Effect of the Recombinant Lectin from Tepary Bean (Phaseolus acutifolius) in Colon Cancer Cells. Pharmaceuticals, 16(2), 290. Distributed under Open Access license CC BY 4.0, without modification.

Mouse Anti-CYCS Recombinant Antibody (CBYY-C2386) (CBMAB-C3824-YY) in WB

The exosome markers including HSP70, TSG101, CD9 and CD63 were measured by western blot in both MLE-12 and mouse BALFs. Cytochrome C as mitochondrial marker, was measured the same method. ...View More

Jang, S., Lee, H., Park, J., Cha, S. R., Lee, J., Park, Y., ... & Yang, S. R. (2023). PTD-FGF2 Attenuates Elastase Induced Emphysema in Mice and Alveolar Epithelial Cell Injury. COPD: Journal of Chronic Obstructive Pulmonary Disease, 20(1), 109-118. Distributed under Open Access license CC BY 4.0, without modification.

Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

CBYY-C2386

Application

WB, IP, IF, IHC-P

Immunogen

Full length denatured cytochrome c of human.

Host Species

Mouse

Specificity

Human, Mouse, Rat, Horse, Chicken

Antibody Isotype

IgG2b, κ

Clonality

Monoclonal Antibody

Application Notes

Application	Note
WB	1:100-1:1,000
IP	1-2 μg per 100-500 μg of total protein (1 mL of cell lysate)
IF(ICC)	1:50-1:500
IHC-P	1:50-1:500

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS, 0.1% gelatin

Preservative

< 0.1% sodium azide

Concentration

0.2 mg/ml

More Infomation

Target

Full Name

Cytochrome C, Somatic

Entrez Gene ID

Human	54205
Mouse	13063
Rat	25309

UniProt ID

Human	P99999
Mouse	P62897
Rat	P62898

Alternative Names

Cytochrome C, Somatic; CYC; Cytochrome C; THC4; HCS;

Function

Electron carrier protein. The oxidized form of the cytochrome c heme group can accept an electron from the heme group of the cytochrome c1 subunit of cytochrome reductase. Cytochrome c then transfers this electron to the cytochrome oxidase complex, the final protein carrier in the mitochondrial electron-transport chain.

Plays a role in apoptosis. Suppression of the anti-apoptotic members or activation of the pro-apoptotic members of the Bcl-2 family leads to altered mitochondrial membrane permeability resulting in release of cytochrome c into the cytosol. Binding of cytochrome c to Apaf-1 triggers the activation of caspase-9, which then accelerates apoptosis by activating other caspases.

Biological Process

Activation of cysteine-type endopeptidase activity involved in apoptotic process by cytochrome c Source: UniProtKB
Apoptotic process Source: UniProtKB-KW
Cellular respiration Source: UniProtKB
Cellular response to oxidative stress Source: Reactome
Mitochondrial electron transport, cytochrome c to oxygen Source: GO_Central
Mitochondrial electron transport, ubiquinol to cytochrome c Source: GO_Central
Mitochondrion organization Source: Reactome
Positive regulation of cysteine-type endopeptidase activity involved in apoptotic process Source: GO_Central

Cellular Location

Mitochondrion intermembrane space. Loosely associated with the inner membrane.

Involvement in disease

Thrombocytopenia 4 (THC4):
A form of thrombocytopenia, a hematologic disorder defined by a decrease in the number of platelets in circulating blood, resulting in the potential for increased bleeding and decreased ability for clotting.

PTM

Binds 1 heme group per subunit.
Phosphorylation at Tyr-49 and Tyr-98 both reduce by half the turnover in the reaction with cytochrome c oxidase, down-regulating mitochondrial respiration.

Liang, Y. D., Zhao, K., Chen, Y., & Zhang, S. Q. (2020). Role of CYCS in the cytogenesis and apoptosis of male germ cells and its clinical application. Zhonghua nan ke xue= National Journal of Andrology, 26(3), 265-270.

Li, L., Sun, Y., Zhang, N., Qiu, X., Wang, L., & Luo, Q. (2019). By regulating miR-182-5p/BCL10/CYCS, sufentanil reduces the apoptosis of umbilical cord mesenchymal stem cells caused by ropivacaine. Bioscience Trends, 13(1), 49-57.

Uchiyama, Y., Yanagisawa, K., Kunishima, S., Shiina, M., Ogawa, Y., Nakashima, M., ... & Mizuguchi, T. (2018). A novel CYCS mutation in the α‐helix of the CYCS C‐terminal domain causes non‐syndromic thrombocytopenia. Clinical Genetics, 94(6), 548-553.

Ong, L., McDonald, K. O., & Ledgerwood, E. C. (2017). Differentiation and cell density upregulate cytochrome c levels in megakaryoblastic cell lines: Implications for analysis of CYCS-associated thrombocytopenia. PloS one, 12(12), e0190433.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

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