Mouse Anti-CYP24A1 Recombinant Antibody (CBFYC-2569) (V2LY-0125-LY932)

Name: Mouse Anti-CYP24A1 Recombinant Antibody (CBFYC-2569)
SKU: V2LY-0125-LY932
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Published Data

Mouse Anti-CYP24A1 Recombinant Antibody (CBFYC-2569) (V2LY-0125-LY932) in WB

Cell lysates were subject to immunoblot analysis against indicated proteins. ...View More

Hsieh Li, S. M., Liu, S. T., Chang, Y. L., Chen, G. S., & Huang, S. M. (2022). Identification of Agents That Ameliorate Hyperphosphatemia-Suppressed Myogenin Expression Involved in the Nrf2/p62 Pathway in C2C12 Skeletal Muscle Cells. International Journal of Molecular Sciences, 23(23), 15324. Distributed under Open Access license CC BY 4.0, without modification.

Mouse Anti-CYP24A1 Recombinant Antibody (CBFYC-2569) (V2LY-0125-LY932) in WB

BEAS-2B cells were transfected with STAT6 siRNA or treated with IL4 (STAT6 inducer) to suppress or induce STAT6 signaling pathway, respectively. The results showed that CYP24A (VDR's target gene) and autophagy-related genes were dose dependent inhibited along with STAT6 interference. ...View More

Yang, Y., Li, Q., Wei, S., Chu, K., Xue, L., Liu, J., ... & Tao, S. (2022). STAT6/VDR Axis Mitigates Lung Inflammatory Injury by Promoting Nrf2 Signaling Pathway. Oxidative Medicine and Cellular Longevity, 2022(1), 2485250. Distributed under Open Access license CC BY 4.0, without modification.

Mouse Anti-CYP24A1 Recombinant Antibody (CBFYC-2569) (V2LY-0125-LY932) in WB

Knockdown of VDR in the human keratinocytes cell line (HPEKp) suppressed IL-33 and ST2 induction by 1,25(OH)2D3. (A,B) VDR (A), CYP24A1 (B), expression 72 h after siRNA tranfecton was measured by real-time PCR in HPEKp cell line. After positive siRNA transfection, IL-33 (D) and ST2 (E) mRNA expression was analysed with or without 1,25(OH)2D3 (100 nM) stimulation. Immunoblotting for VDR, CYP24 (C), IL-33 and ST2 (F) was performed on lysate from HPEKp WT and siVDR cells with or without 1,25(OH)2D3 treatment for 24 h. Blots were stripped and re-probed with anti-β-actin antibody to normalize for differences in protein loading. ...View More

Wierzbicka, J. M., Piotrowska, A., Purzycka-Bohdan, D., Olszewska, A., Nowak, J. I., Szczerkowska-Dobosz, A., ... & Żmijewski, M. A. (2021). The effects of vitamin D on the expression of IL-33 and its receptor ST2 in skin cells; potential implication for psoriasis. International Journal of Molecular Sciences, 22(23), 12907. Distributed under Open Access license CC BY 4.0, without modification.

Mouse Anti-CYP24A1 Recombinant Antibody (CBFYC-2569) (V2LY-0125-LY932) in IHC

CYP24A1 expression a. No significant difference in CYP24A1 mRNA levels was observed between COPD explant tissue and tissue from unused donor lungs (p = 0.07). b. CYP24A1 protein expression was not altered in COPD explant tissue compared to tissue from unused donor lungs. CYP24A1 staining in bronchial epithelium of an unused donor lung (c) and COPD explant lung (d). Positive endothelial staining in an unused donor lung (e) and COPD explant lung (f). Positive immune cell staining in an unused donor lung (g) and COPD explant lung (h). Representative images are shown. N = 10 Horizontal line represents the median value ...View More

Mathyssen, C., Aelbrecht, C., Serré, J., Everaerts, S., Maes, K., Gayan-Ramirez, G., ... & Janssens, W. (2020). Local expression profiles of vitamin D-related genes in airways of COPD patients. Respiratory Research, 21, 1-10. Distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

Datasheet

SDS

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Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

CBFYC-2569

Application

WB, IP, IF, ELISA

Immunogen

Amino acids 351-437 mapping near the C-terminus of human CYP24.

Host Species

Mouse

Specificity

Human

Antibody Isotype

IgG1, κ

Clonality

Monoclonal Antibody

Application Notes

Application	Note
WB	1:100-1:1,000
IP	1-2 μg per 100-500 μg of total protein (1 mL of cell lysate)
IF(ICC)	1:50-1:500
ELISA	1:100-1:1,000

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

Gelatin & PBS

Preservative

Sodium Azide

Concentration

0.2 mg/mL

Purity

>95% as determined by analysis by SDS-PAGE

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

More Infomation

Target

Full Name

Cytochrome P450 Family 24 Subfamily A Member 1

Entrez Gene ID

1591

UniProt ID

Q07973

Function

A cytochrome P450 monooxygenase with a key role in vitamin D catabolism and calcium homeostasis. Via C24- and C23-oxidation pathways, catalyzes the inactivation of both the vitamin D precursor calcidiol (25-hydroxyvitamin D3) and the active hormone calcitriol (1-alpha,25-dihydroxyvitamin D3) (PubMed:24893882, PubMed:15574355, PubMed:8679605, PubMed:11012668, PubMed:16617161, PubMed:29461981).

With initial hydroxylation at C-24 (via C24-oxidation pathway), performs a sequential 6-step oxidation of calcitriol leading to the formation of the biliary metabolite calcitroic acid (PubMed:24893882, PubMed:15574355).

With initial hydroxylation at C-23 (via C23-oxidation pathway), catalyzes sequential oxidation of calcidiol leading to the formation of 25(OH)D3-26,23-lactone as end product (PubMed:11012668, PubMed:8679605).

Preferentially hydroxylates at C-25 other vitamin D active metabolites, such as CYP11A1-derived secosteroids 20S-hydroxycholecalciferol and 20S,23-dihydroxycholecalciferol (PubMed:25727742).

Mechanistically, uses molecular oxygen inserting one oxygen atom into a substrate, and reducing the second into a water molecule, with two electrons provided by NADPH via FDXR/adrenodoxin reductase and FDX1/adrenodoxin (PubMed:8679605).

Biological Process

Fatty acid omega-oxidation Source: GO_Central
Osteoblast differentiation Source: BHF-UCL
Response to vitamin D Source: BHF-UCL
Vitamin D catabolic process Source: UniProtKB
Vitamin D metabolic process Source: Reactome
Vitamin D receptor signaling pathway Source: BHF-UCL
Vitamin metabolic process Source: Reactome

Cellular Location

Mitochondrion

Involvement in disease

Hypercalcemia, infantile, 1 (HCINF1):
A disorder characterized by abnormally high level of calcium in the blood, failure to thrive, vomiting, dehydration, and nephrocalcinosis.

Yasuda, K., Nishikawa, M., Okamoto, K., Horibe, K., Mano, H., Yamaguchi, M., ... & Sakaki, T. (2021). Elucidation of metabolic pathways of 25-hydroxyvitamin D3 mediated by CYP24A1 and CYP3A using Cyp24a1 knockout rats generated by CRISPR/Cas9 system. Journal of Biological Chemistry, 296.

Meyer, M. B., & Pike, J. W. (2020). Mechanistic homeostasis of vitamin D metabolism in the kidney through reciprocal modulation of Cyp27b1 and Cyp24a1 expression. The Journal of steroid biochemistry and molecular biology, 196, 105500.

Cavalier, E., Huyghebaert, L., Rousselle, O., Bekaert, A. C., Kovacs, S., Vranken, L., ... & Ladang, A. (2020). Simultaneous measurement of 25 (OH)-vitamin D and 24, 25 (OH) 2-vitamin D to define cut-offs for CYP24A1 mutation and vitamin D deficiency in a population of 1200 young subjects. Clinical Chemistry and Laboratory Medicine (CCLM), 58(2), 197-201.

Cappellani, D., Brancatella, A., Kaufmann, M., Minucci, A., Vignali, E., Canale, D., ... & Marcocci, C. (2019). Hereditary hypercalcemia caused by a homozygous pathogenic variant in the CYP24A1 gene: a case report and review of the literature. Case Reports in Endocrinology, 2019.

Cai, H., Jiao, Y., Li, Y., Yang, Z., He, M., & Liu, Y. (2019). Low CYP24A1 mRNA expression and its role in prognosis of breast cancer. Scientific reports, 9(1), 1-11.

Sun, H., Jiang, C., Cong, L., Wu, N., Wang, X., Hao, M., ... & Cong, X. (2018). CYP24A1 Inhibition facilitates the antiproliferative effect of 1, 25 (OH) 2D3 through downregulation of the WNT/β-Catenin pathway and methylation-mediated regulation of CYP24A1 in colorectal cancer cells. DNA and Cell Biology, 37(9), 742-749.

Agnello, L., Scazzone, C., Lo Sasso, B., Ragonese, P., Milano, S., Salemi, G., & Ciaccio, M. (2018). CYP27A1, CYP24A1, and RXR-α Polymorphisms, Vitamin D, and multiple sclerosis: a pilot study. Journal of Molecular Neuroscience, 66(1), 77-84.

Pronicka, E., Ciara, E., Halat, P., Janiec, A., Wójcik, M., Rowińska, E., ... & Litwin, M. (2017). Biallelic mutations in CYP24A1 or SLC34A1 as a cause of infantile idiopathic hypercalcemia (IIH) with vitamin D hypersensitivity: molecular study of 11 historical IIH cases. Journal of applied genetics, 58(3), 349-353.

Carpenter, T. O. (2017). CYP24A1 loss of function: clinical phenotype of monoallelic and biallelic mutations. The Journal of steroid biochemistry and molecular biology, 173, 337-340.

Hawkes, C. P., Li, D., Hakonarson, H., Meyers, K. E., Thummel, K. E., & Levine, M. A. (2017). CYP3A4 induction by rifampin: an alternative pathway for vitamin D inactivation in patients with CYP24A1 mutations. The Journal of Clinical Endocrinology & Metabolism, 102(5), 1440-1446.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

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