Mouse Anti-CYP2B6 Recombinant Antibody (CBXC-2178) (V2LY-0125-LY951)

Name: Mouse Anti-CYP2B6 Recombinant Antibody (CBXC-2178)
SKU: V2LY-0125-LY951
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

CBXC-2178

Application

WB, IHC, IF, FC

Immunogen

Full length human recombinant protein of human CYP2B6.

Host Species

Mouse

Specificity

Human, Dog, Rat, Monkey, Mouse

Antibody Isotype

IgG2a

Clonality

Monoclonal Antibody

Application Notes

Application	Note
WB	1:500-1:2,000
IHC	1:150
IF(ICC)	1:100
FC	1:100

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

BSA, Glycerol & PBS

Preservative

Sodium Azide

Concentration

1 mg/mL

Purity

>95% as determined by analysis by SDS-PAGE

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

More Infomation

Target

Full Name

Cytochrome P450 Family 2 Subfamily B Member 6

Entrez Gene ID

Human	1555
Mouse	105869130
Rat	286953
Monkey	678687
Dog	474177

UniProt ID

Human	P20813
Rat	G5C9V9
Monkey	B8K1S9
Dog	F1PNK2

Function

A cytochrome P450 monooxygenase involved in the metabolism of endocannabinoids and steroids (PubMed:21289075, PubMed:12865317).

Mechanistically, uses molecular oxygen inserting one oxygen atom into a substrate, and reducing the second into a water molecule, with two electrons provided by NADPH via cytochrome P450 reductase (NADPH--hemoprotein reductase). Catalyzes the epoxidation of double bonds of arachidonoylethanolamide (anandamide) to 8,9-, 11,12-, and 14,15-epoxyeicosatrienoic acid ethanolamides (EpETrE-EAs), potentially modulating endocannabinoid system signaling (PubMed:21289075).

Hydroxylates steroid hormones, including testosterone at C-16 and estrogens at C-2 (PubMed:21289075, PubMed:12865317).

Plays a role in the oxidative metabolism of xenobiotics, including plant lipids and drugs (PubMed:11695850, PubMed:22909231).

Acts as a 1,4-cineole 2-exo-monooxygenase (PubMed:11695850).

Allele 2B6*9: Has low affinity for anandamide and can only produce 11,12 EpETrE-EAs.

Biological Process

Cellular ketone metabolic process Source: BHF-UCL
Drug metabolic process Source: BHF-UCL
Epoxygenase P450 pathway Source: GO_Central
Exogenous drug catabolic process Source: BHF-UCL
Organic acid metabolic process Source: GO_Central
Steroid metabolic process Source: BHF-UCL
Xenobiotic metabolic process Source: UniProtKB

Cellular Location

Endoplasmic reticulum membrane; Microsome membrane

PTM

Phosphorylation is accompanied by a decrease in enzyme activity.

Mangó, K., Kiss, Á. F., Fekete, F., Erdős, R., & Monostory, K. (2022). CYP2B6 allelic variants and non-genetic factors influence CYP2B6 enzyme function. Scientific reports, 12(1), 1-14.

Desta, Z., El‐Boraie, A., Gong, L., Somogyi, A. A., Lauschke, V. M., Dandara, C., ... & Gaedigk, A. (2021). PharmVar GeneFocus: CYP2B6. Clinical Pharmacology & Therapeutics, 110(1), 82-97.

Griesel, R., Maartens, G., Chirehwa, M., Sokhela, S., Akpomiemie, G., Moorhouse, M., ... & Sinxadi, P. (2021). CYP2B6 genotype and weight gain differences between dolutegravir and efavirenz. Clinical Infectious Diseases, 73(11), e3902-e3909.

Langmia, I. M., Just, K. S., Yamoune, S., Brockmöller, J., Masimirembwa, C., & Stingl, J. C. (2021). CYP2B6 Functional Variability in Drug Metabolism and Exposure Across Populations—Implication for Drug Safety, Dosing, and Individualized Therapy. Frontiers in Genetics, 12, 1205.

Marok, F. Z., Fuhr, L. M., Hanke, N., Selzer, D., & Lehr, T. (2021). Physiologically based pharmacokinetic modeling of bupropion and its metabolites in a CYP2B6 drug-drug-gene interaction network. Pharmaceutics, 13(3), 331.

Helsby, N. A., Yong, M., van Kan, M., de Zoysa, J. R., & Burns, K. E. (2019). The importance of both CYP2C19 and CYP2B6 germline variations in cyclophosphamide pharmacokinetics and clinical outcomes. British Journal of Clinical Pharmacology, 85(9), 1925-1934.

Desta, Z., Gammal, R. S., Gong, L., Whirl‐Carrillo, M., Gaur, A. H., Sukasem, C., ... & Haas, D. W. (2019). Clinical pharmacogenetics implementation consortium (CPIC) guideline for CYP2B6 and efavirenz‐containing antiretroviral therapy. Clinical Pharmacology & Therapeutics, 106(4), 726-733.

Kharasch, E. D., & Crafford, A. (2019). Common polymorphisms of CYP2B6 influence stereoselective bupropion disposition. Clinical Pharmacology & Therapeutics, 105(1), 142-152.

Burgess, K. S., Ipe, J., Swart, M., Metzger, I. F., Lu, J., Gufford, B. T., ... & Skaar, T. C. (2018). Variants in the CYP2B6 3′ UTR alter in vitro and in vivo CYP2B6 activity: potential role of microRNAs. Clinical Pharmacology & Therapeutics, 104(1), 130-138.

Ahmad, T., Sabet, S., Primerano, D. A., Richards-Waugh, L. L., & Rankin, G. O. (2017). Tell-tale SNPs: the role of CYP2B6 in methadone fatalities. Journal of analytical toxicology, 41(4), 325-333.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

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We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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