Human CYP2B6 ELISA Kit (V2LY-0626-LY3622)

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Tested Data
Datasheet
SDS
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Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Sensitivity
0.0098 ng/mL
Detection Range
0.02-4.5 ng/mL
Sample Type
Serum, Plasma, cell culture supernates
Specificity
Human
Assay Type
Sandwich
Reactivity
Human
Assay Time
1.5 h
Molecule Mass
56.3 kDa
Components
  • Pre-coated ELISA Plate: 12 wells * 8 detachable strips
  • Standard solution: 0.5ml x1
  • Standard diluent: 3ml x1
  • Streptavidin-HRP: 6ml x1
  • Stop solution: 6ml x1
  • Substrate solution A: 6ml x1
  • Substrate solution B: 6ml x1
  • Wash buffer concentrate (25x): 20ml x1
  • Biotinylated antibody: 1ml x1

Formulations & Storage [For reference only, actual COA shall prevail!]

Storage
Store at 2-8°C
More Infomation

Target

Full Name
Cytochrome P450 Family 2 Subfamily B Member 6
Function
A cytochrome P450 monooxygenase involved in the metabolism of endocannabinoids and steroids (PubMed:21289075, PubMed:12865317).

Mechanistically, uses molecular oxygen inserting one oxygen atom into a substrate, and reducing the second into a water molecule, with two electrons provided by NADPH via cytochrome P450 reductase (NADPH--hemoprotein reductase). Catalyzes the epoxidation of double bonds of arachidonoylethanolamide (anandamide) to 8,9-, 11,12-, and 14,15-epoxyeicosatrienoic acid ethanolamides (EpETrE-EAs), potentially modulating endocannabinoid system signaling (PubMed:21289075).

Hydroxylates steroid hormones, including testosterone at C-16 and estrogens at C-2 (PubMed:21289075, PubMed:12865317).

Plays a role in the oxidative metabolism of xenobiotics, including plant lipids and drugs (PubMed:11695850, PubMed:22909231).

Acts as a 1,4-cineole 2-exo-monooxygenase (PubMed:11695850).

Allele 2B6*9: Has low affinity for anandamide and can only produce 11,12 EpETrE-EAs.
Biological Process
Cellular ketone metabolic process Source: BHF-UCL
Drug metabolic process Source: BHF-UCL
Epoxygenase P450 pathway Source: GO_Central
Exogenous drug catabolic process Source: BHF-UCL
Organic acid metabolic process Source: GO_Central
Steroid metabolic process Source: BHF-UCL
Xenobiotic metabolic process Source: UniProtKB
Cellular Location
Endoplasmic reticulum membrane; Microsome membrane
PTM
Phosphorylation is accompanied by a decrease in enzyme activity.

Mangó, K., Kiss, Á. F., Fekete, F., Erdős, R., & Monostory, K. (2022). CYP2B6 allelic variants and non-genetic factors influence CYP2B6 enzyme function. Scientific reports, 12(1), 1-14.

Desta, Z., El‐Boraie, A., Gong, L., Somogyi, A. A., Lauschke, V. M., Dandara, C., ... & Gaedigk, A. (2021). PharmVar GeneFocus: CYP2B6. Clinical Pharmacology & Therapeutics, 110(1), 82-97.

Griesel, R., Maartens, G., Chirehwa, M., Sokhela, S., Akpomiemie, G., Moorhouse, M., ... & Sinxadi, P. (2021). CYP2B6 genotype and weight gain differences between dolutegravir and efavirenz. Clinical Infectious Diseases, 73(11), e3902-e3909.

Langmia, I. M., Just, K. S., Yamoune, S., Brockmöller, J., Masimirembwa, C., & Stingl, J. C. (2021). CYP2B6 Functional Variability in Drug Metabolism and Exposure Across Populations—Implication for Drug Safety, Dosing, and Individualized Therapy. Frontiers in Genetics, 12, 1205.

Marok, F. Z., Fuhr, L. M., Hanke, N., Selzer, D., & Lehr, T. (2021). Physiologically based pharmacokinetic modeling of bupropion and its metabolites in a CYP2B6 drug-drug-gene interaction network. Pharmaceutics, 13(3), 331.

Helsby, N. A., Yong, M., van Kan, M., de Zoysa, J. R., & Burns, K. E. (2019). The importance of both CYP2C19 and CYP2B6 germline variations in cyclophosphamide pharmacokinetics and clinical outcomes. British Journal of Clinical Pharmacology, 85(9), 1925-1934.

Desta, Z., Gammal, R. S., Gong, L., Whirl‐Carrillo, M., Gaur, A. H., Sukasem, C., ... & Haas, D. W. (2019). Clinical pharmacogenetics implementation consortium (CPIC) guideline for CYP2B6 and efavirenz‐containing antiretroviral therapy. Clinical Pharmacology & Therapeutics, 106(4), 726-733.

Kharasch, E. D., & Crafford, A. (2019). Common polymorphisms of CYP2B6 influence stereoselective bupropion disposition. Clinical Pharmacology & Therapeutics, 105(1), 142-152.

Burgess, K. S., Ipe, J., Swart, M., Metzger, I. F., Lu, J., Gufford, B. T., ... & Skaar, T. C. (2018). Variants in the CYP2B6 3′ UTR alter in vitro and in vivo CYP2B6 activity: potential role of microRNAs. Clinical Pharmacology & Therapeutics, 104(1), 130-138.

Ahmad, T., Sabet, S., Primerano, D. A., Richards-Waugh, L. L., & Rankin, G. O. (2017). Tell-tale SNPs: the role of CYP2B6 in methadone fatalities. Journal of analytical toxicology, 41(4), 325-333.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

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