Mouse Anti-CYP7A1 Recombinant Antibody (CBCNC-574) (V2LY-0125-LY1012)
Basic Information
| Application | Note |
| IF(ICC) | 1-25 μg/mL |
Formulations & Storage [For reference only, actual COA shall prevail!]
Target
Mechanistically, uses molecular oxygen inserting one oxygen atom into a substrate, and reducing the second into a water molecule, with two electrons provided by NADPH via cytochrome P450 reductase (CPR; NADPH-ferrihemoprotein reductase) (PubMed:2384150, PubMed:11013305, PubMed:12077124, PubMed:19965590, PubMed:21813643).
Functions as a critical regulatory enzyme of bile acid biosynthesis and cholesterol homeostasis. Catalyzes the hydroxylation of carbon hydrogen bond at 7-alpha position of cholesterol, a rate-limiting step in cholesterol catabolism and bile acid biosynthesis (PubMed:12077124, PubMed:19965590, PubMed:2384150).
7-alpha hydroxylates several oxysterols, including 4beta-hydroxycholesterol and 24-hydroxycholesterol (PubMed:11013305, PubMed:12077124).
Catalyzes the oxidation of the 7,8 double bond of 7-dehydrocholesterol and lathosterol with direct and predominant formation of the 7-keto derivatives (PubMed:21813643).
Bile acid biosynthetic process Source: UniProtKB
Bile acid signaling pathway Source: Ensembl
Cellular response to cholesterol Source: UniProtKB
Cellular response to glucose stimulus Source: UniProtKB
Cholesterol catabolic process Source: UniProtKB
Cholesterol homeostasis Source: UniProtKB
Negative regulation of collagen biosynthetic process Source: Ensembl
Negative regulation of fatty acid biosynthetic process Source: Ensembl
Positive regulation of cholesterol biosynthetic process Source: Ensembl
Regulation of bile acid biosynthetic process Source: UniProtKB
Regulation of gene expression Source: Ensembl
Regulation of metabolic process Source: Reactome
Response to ethanol Source: Ensembl
Sterol metabolic process Source: Reactome
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Please try the standard protocols which include: protocols, troubleshooting and guide.
Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols
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Mouse Anti-CYP7A1 Recombinant Antibody (CBYY-C0730) (CAT#: V2LY-0125-LY1009)
Mouse Anti-CYP7A1 Recombinant Antibody (CBYY-C2400) (CAT#: V2LY-0125-LY1010)
Custom Antibody Labeling
We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).
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