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Mouse Anti-CYP7A1 Recombinant Antibody (CBYY-C2400) (V2LY-0125-LY1010)


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Summary

Host Animal
Mouse
Specificity
Human, Mouse, Rat
Clone
CBYY-C2400
Antibody Isotype
IgG1
Application
WB

Basic Information

Immunogen
Purified recombinant human CYP7A1(C-term) protein fragments expressed in E Coli.
Host Species
Mouse
Specificity
Human, Mouse, Rat
Antibody Isotype
IgG1
Clonality
Monoclonal Antibody

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
Glycerol & Tris-Glycine
Preservative
Sodium Azide
Concentration
Batch dependent
Purity
>95% as determined by analysis by SDS-PAGE
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Target

Full Name
Cytochrome P450 Family 7 Subfamily A Member 1
Entrez Gene ID
Human1581
Mouse13122
Rat25428
UniProt ID
HumanP22680
MouseQ64505
RatP18125
Function
A cytochrome P450 monooxygenase involved in the metabolism of endogenous cholesterol and its oxygenated derivatives (oxysterols) (PubMed:11013305, PubMed:12077124, PubMed:19965590, PubMed:2384150, PubMed:21813643).

Mechanistically, uses molecular oxygen inserting one oxygen atom into a substrate, and reducing the second into a water molecule, with two electrons provided by NADPH via cytochrome P450 reductase (CPR; NADPH-ferrihemoprotein reductase) (PubMed:2384150, PubMed:11013305, PubMed:12077124, PubMed:19965590, PubMed:21813643).

Functions as a critical regulatory enzyme of bile acid biosynthesis and cholesterol homeostasis. Catalyzes the hydroxylation of carbon hydrogen bond at 7-alpha position of cholesterol, a rate-limiting step in cholesterol catabolism and bile acid biosynthesis (PubMed:12077124, PubMed:19965590, PubMed:2384150).

7-alpha hydroxylates several oxysterols, including 4beta-hydroxycholesterol and 24-hydroxycholesterol (PubMed:11013305, PubMed:12077124).

Catalyzes the oxidation of the 7,8 double bond of 7-dehydrocholesterol and lathosterol with direct and predominant formation of the 7-keto derivatives (PubMed:21813643).
Biological Process
Bile acid and bile salt transport Source: Ensembl
Bile acid biosynthetic process Source: UniProtKB
Bile acid signaling pathway Source: Ensembl
Cellular response to cholesterol Source: UniProtKB
Cellular response to glucose stimulus Source: UniProtKB
Cholesterol catabolic process Source: UniProtKB
Cholesterol homeostasis Source: UniProtKB
Negative regulation of collagen biosynthetic process Source: Ensembl
Negative regulation of fatty acid biosynthetic process Source: Ensembl
Positive regulation of cholesterol biosynthetic process Source: Ensembl
Regulation of bile acid biosynthetic process Source: UniProtKB
Regulation of gene expression Source: Ensembl
Regulation of metabolic process Source: Reactome
Response to ethanol Source: Ensembl
Sterol metabolic process Source: Reactome
Cellular Location
Endoplasmic reticulum membrane; Microsome membrane
Topology
Helical: 4-24
More Infomation

Cao, K., Zhang, K., Ma, M., Ma, J., Tian, J., & Jin, Y. (2021). Lactobacillus mediates the expression of NPC1L1, CYP7A1, and ABCG5 genes to regulate cholesterol. Food Science & Nutrition, 9(12), 6882-6891.

Iwanicki, T., Balcerzyk, A., Niemiec, P., Trautsolt, W., Grzeszczak, W., Ochalska-Tyka, A., ... & Żak, I. (2019). The relationship between CYP7A1 polymorphisms, coronary artery disease & serum lipid markers. Biomarkers in Medicine, 13(14), 1199-1208.

Chambers, K. F., Day, P. E., Aboufarrag, H. T., & Kroon, P. A. (2019). Polyphenol effects on cholesterol metabolism via bile acid biosynthesis, CYP7A1: a review. Nutrients, 11(11), 2588.

Duan, Y., Zhang, F., Yuan, W., Wei, Y., Wei, M., Zhou, Y., ... & Wu, X. (2019). Hepatic cholesterol accumulation ascribed to the activation of ileum Fxr-Fgf15 pathway inhibiting hepatic Cyp7a1 in high-fat diet-induced obesity rats. Life sciences, 232, 116638.

Zhang, T., Zhao, M., Lu, D., Wang, S., Yu, F., Guo, L., ... & Wu, B. (2018). REV-ERBα regulates CYP7A1 through repression of liver receptor homolog-1. Drug Metabolism and Disposition, 46(3), 248-258.

Qayyum, F., Lauridsen, B. K., Frikke-Schmidt, R., Kofoed, K. F., Nordestgaard, B. G., & Tybjærg-Hansen, A. (2018). Genetic variants in CYP7A1 and risk of myocardial infarction and symptomatic gallstone disease. European Heart Journal, 39(22), 2106-2116.

Wang, D., Hartmann, K., Seweryn, M., & Sadee, W. (2018). Interactions between regulatory variants in CYP7A1 (cholesterol 7α-hydroxylase) promoter and enhancer regions regulate CYP7A1 expression. Circulation: Genomic and Precision Medicine, 11(10), e002082.

Enya, S., Kawakami, K., Suzuki, Y., & Kawaoka, S. (2018). A novel zebrafish intestinal tumor model reveals a role for cyp7a1-dependent tumor–liver crosstalk in causing adverse effects on the host. Disease models & mechanisms, 11(8), dmm032383.

Abdullah, M. M., Eck, P. K., Couture, P., Lamarche, B., & Jones, P. J. (2018). The combination of single nucleotide polymorphisms rs6720173 (ABCG5), rs3808607 (CYP7A1), and rs760241 (DHCR7) is associated with differing serum cholesterol responses to dairy consumption. Applied Physiology, Nutrition, and Metabolism, 43(10), 1090-1093.

Wang, Y., Ding, Y., Li, J., Chavan, H., Matye, D., Ni, H. M., ... & Li, T. (2017). Targeting the enterohepatic bile acid signaling induces hepatic autophagy via a CYP7A1–AKT–mTOR axis in mice. Cellular and molecular gastroenterology and hepatology, 3(2), 245-260.

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For research use only. Not intended for any clinical use.

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