Mouse Anti-CYP8B1 Recombinant Antibody (CBWJC-2112) (V2LY-0125-LY1017)

Name: Mouse Anti-CYP8B1 Recombinant Antibody (CBWJC-2112)
SKU: V2LY-0125-LY1017
Rating: 5 (1 reviews)

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Published Data

Mouse Anti-CYP8B1 Recombinant Antibody (CBWJC-2112) (V2LY-0125-LY1017) in IP

Co-IP of PKA-C and PKA-R with PER1 and CYP8B1 in WT and Per1 KO mice. Reciprocal Co-IP was conducted using PER1 or CYP8B1 antibody for IP, respectively. n=3 per group. ...View More

Ge, W., Sun, Q., Yang, Y., Ding, Z., Liu, J., & Zhang, J. (2023). Circadian PER1 controls daily fat absorption with the regulation of PER1-PKA on phosphorylation of bile acid synthetase. Journal of Lipid Research, 64(6), 100390. Distributed under Open Access license CC BY 4.0, without modification.

Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

CBWJC-2112

Application

WB, ELISA, IP

Immunogen

Amino acids 486-494 of human CYP8B1.

Host Species

Mouse

Specificity

Human, Mouse, Rat

Antibody Isotype

IgG1, κ

Clonality

Monoclonal Antibody

Application Notes

Application	Note
WB	1:100-1:1,000
IP	1-2 μg per 100-500 μg of total protein (1 mL of cell lysate)
ELISA	1:100-1:1,000

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

Gelatin & PBS

Preservative

Sodium Azide

Concentration

0.2 mg/mL

Purity

>95% as determined by analysis by SDS-PAGE

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

More Infomation

Target

Full Name

Cytochrome P450 Family 8 Subfamily B Member 1

Entrez Gene ID

Human	1582
Rat	81924

UniProt ID

Human	Q9UNU6
Rat	Q9WVT5

Function

A cytochrome P450 monooxygenase involved in primary bile acid biosynthesis. Catalyzes the 12alpha-hydroxylation of 7alpha-hydroxy-4-cholesten-3-one, an intermediate metabolite in cholic acid biosynthesis (PubMed:10051404).

Controls biliary balance of cholic acid and chenodeoxycholic acid, ultimately regulating the intestinal absorption of dietary lipids (By similarity).

Mechanistically, uses molecular oxygen inserting one oxygen atom into a substrate, and reducing the second into a water molecule, with two electrons provided by NADPH via cytochrome P450 reductase (CPR; NADPH--hemoprotein reductase) (By similarity).

Biological Process

Bile acid biosynthetic process Source: UniProtKB
Bile acid signaling pathway Source: Ensembl
Positive regulation of intestinal cholesterol absorption Source: UniProtKB
Response to cholesterol Source: Ensembl
Response to nutrient levels Source: Ensembl
Sterol metabolic process Source: Reactome

Cellular Location

Endoplasmic reticulum membrane; Microsome membrane

Topology

Helical: 1-21

Petrov, P. D., Fernández-Murga, L., Conde, I., Martínez-Sena, T., Guzmán, C., Castell, J. V., & Jover, R. (2020). Epistane, an anabolic steroid used for recreational purposes, causes cholestasis with elevated levels of cholic acid conjugates, by upregulating bile acid synthesis (CYP8B1) and cross-talking with nuclear receptors in human hepatocytes. Archives of toxicology, 94(2), 589-607.

Axling, U., Cavalera, M., Degerman, E., Gåfvels, M., Eggertsen, G., & Holm, C. (2020). Increased whole body energy expenditure and protection against diet-induced obesity in Cyp8b1-deficient mice is accompanied by altered adipose tissue features. Adipocyte, 9(1), 587-599.

Fan, L., Joseph, J. F., Durairaj, P., Parr, M. K., & Bureik, M. (2019). Conversion of chenodeoxycholic acid to cholic acid by human CYP8B1. Biological Chemistry, 400(5), 625-628.

Shinde, S. S., Teekas, L., Sharma, S., & Vijay, N. (2019). Signatures of relaxed selection in the CYP8B1 gene of birds and mammals. Journal of Molecular Evolution, 87(7), 209-220.

Chevre, R., Trigueros‐Motos, L., Castaño, D., Chua, T., Corliano, M., Patankar, J. V., ... & Singaraja, R. R. (2018). Therapeutic modulation of the bile acid pool by Cyp8b1 knockdown protects against nonalcoholic fatty liver disease in mice. The FASEB Journal, 32(7), 3792-3802.

Patankar, J. V., Wong, C. K., Morampudi, V., Gibson, W. T., Vallance, B., Ioannou, G. N., & Hayden, M. R. (2018). Genetic ablation of Cyp8b1 preserves host metabolic function by repressing steatohepatitis and altering gut microbiota composition. American Journal of Physiology-Endocrinology and Metabolism, 314(5), E418-E432.

Patankar, J. V., Wong, C. K., Morampudi, V., Gibson, W. T., Vallance, B., Ioannou, G. N., & Hayden, M. R. (2018). Role of Gut Microbiota and Gut-Brain and Gut-Liver Axes in Physiological Regulation of Inflammation, Energy Balance, and Metabolism: Genetic ablation of Cyp8b1 preserves host metabolic function by repressing steatohepatitis and altering gut microbiota composition. American Journal of Physiology-Endocrinology and Metabolism, 314(5), E418.

Bertaggia, E., Jensen, K. K., Castro-Perez, J., Xu, Y., Di Paolo, G., Chan, R. B., ... & Haeusler, R. A. (2017). Cyp8b1 ablation prevents Western diet-induced weight gain and hepatic steatosis because of impaired fat absorption. American Journal of Physiology-Endocrinology and Metabolism, 313(2), E121-E133.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Isotype control

For research use only. Not intended for any clinical use.

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