Mouse Anti-CYTH1 Recombinant Antibody (2E11) (V2LY-0125-LY1023)

Name: Mouse Anti-CYTH1 Recombinant Antibody (2E11)
SKU: V2LY-0125-LY1023
Rating: 5 (1 reviews)

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Datasheet

SDS

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Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

2E11

Application

WB, FC, IF

Immunogen

Purified, His-tagged cytohesin-1.

Host Species

Mouse

Specificity

Human

Antibody Isotype

IgG1

Clonality

Monoclonal Antibody

Application Notes

Application	Note
WB	1 μg/mL
IF(ICC)	1:20
FC	3-5 μg/10⁶ cells

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

BSA & PBS

Preservative

Sodium Azide

Concentration

1 mg/mL

Purity

>95% as determined by analysis by SDS-PAGE

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

More Infomation

Target

Full Name

Cytohesin 1

Entrez Gene ID

9267

UniProt ID

Q15438

Function

Promotes guanine-nucleotide exchange on ARF1, ARF5 and ARF6. Promotes the activation of ARF factors through replacement of GDP with GTP. Plays an important role in membrane trafficking, during junctional remodeling and epithelial polarization, through regulation of ARF6 activity.

Biological Process

Establishment of epithelial cell polarity Source: UniProtKB
Regulation of ARF protein signal transduction Source: InterPro
Regulation of cell adhesion Source: MGI
Vesicle-mediated transport Source: ProtInc

Cellular Location

Cell membrane; Cytosol; Tight junction; Adherens junction. Colocalized with TJP1 during epithelial polarization.

PTM

Ubiquitinated by SCF(FBXW11) E3 ubiquitin-protein ligase complex. Ubiquitination induces proteasomal degradation.

Ratcliffe, C. D., Siddiqui, N., Coelho, P. P., Laterreur, N., Cookey, T. N., Sonenberg, N., & Park, M. (2019). HGF-induced migration depends on the PI (3, 4, 5) P3-binding microexon-spliced variant of the Arf6 exchange factor cytohesin-1. Journal of Cell Biology, 218(1), 285-298.

Rak, J., Foster, K., Potrzebowska, K., Talkhoncheh, M. S., Miharada, N., Komorowska, K., ... & Larsson, J. (2017). Cytohesin 1 regulates homing and engraftment of human hematopoietic stem and progenitor cells. Blood, The Journal of the American Society of Hematology, 129(8), 950-958.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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