Mouse Anti-DAAM1 Recombinant Antibody (5D3) (V2LY-0125-LY1044)

Name: Mouse Anti-DAAM1 Recombinant Antibody (5D3)
SKU: V2LY-0125-LY1044
Rating: 5 (1 reviews)

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Published Data

Mouse Anti-DAAM1 Recombinant Antibody (5D3) (V2LY-0125-LY1044) in WB

DAAM1 and MLC2 protein expression as well as the extent of MLC2 phosphorylation determined by Western blot analysis in mouse tumor tissues. ...View More

Wang, S., Ma, Y., Hu, Y., Zhao, X., Li, Y., Ouyang, S., & Luo, G. (2023). FOXD3 confers chemo-sensitivity in ovarian cancer through a miR-335/DAAM1/myosin II axis-dependent mechanism. Journal of Ovarian Research, 16(1), 8. Distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

Mouse Anti-DAAM1 Recombinant Antibody (5D3) (V2LY-0125-LY1044) in Immunoblotting

Analysis of gonococcal colony internalization, phagocytosis and immunoblot validation of U937 MFs cell lines lacking different formins. ...View More

Ivanov, S. S., Castore, R., Juarez Rodriguez, M. D., Circu, M., & Dragoi, A. M. (2021). Neisseria gonorrhoeae subverts formin-dependent actin polymerization to colonize human macrophages. PloS pathogens, 17(12), e1010184. Distributed under Open Access license CC BY 4.0, without modification.

Mouse Anti-DAAM1 Recombinant Antibody (5D3) (V2LY-0125-LY1044) in IF

MDA-MB-231 cells were stimulated with DMEM or ACM in the presence of DMSO or centrinone+AZD as indicated. Cells were stained for actin and (d) DAAM1, or (e) DAAM2. Bar=20µm. ...View More

Luo, Y., Barrios-Rodiles, M., Gupta, G. D., Zhang, Y. Y., Ogunjimi, A. A., Bashkurov, M., ... & Pelletier, L. (2019). Atypical function of a centrosomal module in WNT signalling drives contextual cancer cell motility. Nature communications, 10(1), 2356. Distributed under Open Access license CC BY 4.0, without modification.

Mouse Anti-DAAM1 Recombinant Antibody (5D3) (V2LY-0125-LY1044) in IF

Indirect immunofluorescence of adult (P60) mouse liver cryosections with anti-Cx26 antibody (green) and CGN, FLNB, and DAAM1 antibodies (all in red). DNA is in a white pseudo color, according to DAPI staining. The analysis was performed at confocal microscopy with z-sections of 0.5 µm (LSM880, Carl Zeiss, Oberkochen, Germany). Each image consists of the maximum intensity projection of all z-sections obtained. Arrowheads: signal at the plasma membrane. Arrows: merged signals. Scale bar: 10 µm. ...View More

Batissoco, A. C., Salazar-Silva, R., Oiticica, J., Bento, R. F., Mingroni-Netto, R. C., & Haddad, L. A. (2018). A cell junctional protein network associated with connexin-26. International journal of molecular sciences, 19(9), 2535. Distributed under Open Access license CC BY 4.0, without modification.

Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

5D3

Application

ELISA, WB, IP

Immunogen

Amino acids 1-111 within an N-terminal region of human DAAM1.

Host Species

Mouse

Specificity

Human, Mouse, Rat

Antibody Isotype

IgG2a, κ

Clonality

Monoclonal Antibody

Application Notes

Application	Note
WB	1:100-1:1,000
IP	1-2 μg per 100-500 μg of total protein (1 mL of cell lysate)
ELISA	1:100-1:1,000

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

Gelatin & PBS

Preservative

Sodium Azide

Concentration

0.1 mg/mL

Purity

>95% as determined by analysis by SDS-PAGE

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

More Infomation

Target

Full Name

dishevelled associated activator of morphogenesis 1

Entrez Gene ID

23002

UniProt ID

Q9Y4D1

Function

Binds to disheveled (Dvl) and Rho, and mediates Wnt-induced Dvl-Rho complex formation. May play a role as a scaffolding protein to recruit Rho-GDP and Rho-GEF, thereby enhancing Rho-GTP formation. Can direct nucleation and elongation of new actin filaments. Involved in building functional cilia (PubMed:16630611, PubMed:17482208).

Involved in the organization of the subapical actin network in multiciliated epithelial cells (By similarity).

Together with DAAM2, required for myocardial maturation and sarcomere assembly (By similarity).

Biological Process

Actin cytoskeleton organization Source: InterPro
Wnt signaling pathway, planar cell polarity pathway Source: Reactome

Cellular Location

Cilium basal body; Cytoplasm. Perinuclear.

Dai, B., Shen, Y., Yan, T., & Zhang, A. (2020). Wnt5a/ROR1 activates DAAM1 and promotes the migration in osteosarcoma cells. Oncology Reports, 43(2), 601-608.

Mei, J., Xu, B., Hao, L., Xiao, Z., Liu, Y., Yan, T., & Zhu, Y. (2020). Overexpressed DAAM1 correlates with metastasis and predicts poor prognosis in breast cancer. Pathology-Research and Practice, 216(3), 152736.

Mei, J., Yan, T., Huang, Y., Xia, T., Chang, F., Shen, S., ... & Zhu, Y. (2019). A DAAM1 3′-UTR SNP mutation regulates breast cancer metastasis through affecting miR-208a-5p-DAAM1-RhoA axis. Cancer cell international, 19(1), 1-12.

Li, M. Y., Peng, W. H., Wu, C. H., Chang, Y. M., Lin, Y. L., Chang, G. D., ... & Chen, G. C. (2019). PTPN3 suppresses lung cancer cell invasiveness by counteracting Src-mediated DAAM1 activation and actin polymerization. Oncogene, 38(44), 7002-7016.

Xiong, H., Yan, T., Zhang, W., Shi, F., Jiang, X., Wang, X., ... & Zhu, Y. (2018). miR-613 inhibits cell migration and invasion by downregulating Daam1 in triple-negative breast cancer. Cellular signalling, 44, 33-42.

Liu, G., Yan, T., Li, X., Sun, J., Zhang, B., Wang, H., & Zhu, Y. (2018). Daam1 activates RhoA to regulate Wnt5a‑induced glioblastoma cell invasion. Oncology Reports, 39(2), 465-472.

Yanai, S., Wakayama, M., Nakayama, H., Shinozaki, M., Tsukuma, H., Tochigi, N., ... & Shibuya, K. (2017). Implication of overexpression of dishevelled-associated activator of morphogenesis 1 (Daam-1) for the pathogenesis of human Idiopathic Pulmonary Arterial Hypertension (IPAH). Diagnostic Pathology, 12(1), 1-11.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

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For research use only. Not intended for any clinical use.

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