Mouse Anti-DACH1 Recombinant Antibody (D0163) (V2LY-0125-LY1056)

Name: Mouse Anti-DACH1 Recombinant Antibody (D0163)
SKU: V2LY-0125-LY1056
Rating: 5 (1 reviews)

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Datasheet

SDS

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Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

D0163

Application

WB, IP, IF, ELISA

Immunogen

Amino acids 528-620 mapping within an internal region of human DACH1.

Host Species

Mouse

Specificity

Human, Mouse, Rat, Cattle, Pig

Antibody Isotype

IgG2a, κ

Clonality

Monoclonal Antibody

Application Notes

Application	Note
WB	1:100-1:1,000
IP	1-2 μg per 100-500 μg of total protein (1 mL of cell lysate)
ELISA	1:100-1:1,000
IF(ICC)	1:50-1:500

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

Gelatin & PBS

Preservative

Sodium Azide

Concentration

0.2 mg/mL

Purity

>95% as determined by analysis by SDS-PAGE

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

More Infomation

Target

Full Name

Dachshund Family Transcription Factor 1

Entrez Gene ID

Human	1602
Mouse	13134
Rat	306096

UniProt ID

Human	Q9UI36
Mouse	Q9QYB2
Rat	F1M0Y6

Function

Transcription factor that is involved in regulation of organogenesis. Seems to be a regulator of SIX1, SIX6 and probably SIX5. Corepression of precursor cell proliferation in myoblasts by SIX1 is switched to coactivation through recruitment of EYA3 to the SIX1-DACH1 complex. Transcriptional activation seems also to involve association of CREBBP. Seems to act as a corepressor of SIX6 in regulating proliferation by directly repressing cyclin-dependent kinase inhibitors, including the p27Kip1 promoter (By similarity).

Inhibits TGF-beta signaling through interaction with SMAD4 and NCOR1. Binds to chromatin DNA via its DACHbox-N domain (By similarity).

Biological Process

Cell population proliferation Source: Ensembl
Development of primary female sexual characteristics Source: Ensembl
Negative regulation of cell migration Source: BHF-UCL
Negative regulation of cell proliferation involved in contact inhibition Source: Ensembl
Negative regulation of DNA biosynthetic process Source: CACAO
Negative regulation of fibroblast proliferation Source: Ensembl
Negative regulation of transcription, DNA-templated Source: CACAO
Negative regulation of transcription by competitive promoter binding Source: BHF-UCL
Negative regulation of transcription by RNA polymerase II Source: BHF-UCL
Regulation of nuclear cell cycle DNA replication Source: Ensembl
Regulation of transcription by RNA polymerase II Source: GO_Central
Respiratory gaseous exchange by respiratory system Source: Ensembl
Suckling behavior Source: Ensembl

Cellular Location

Nucleus

Zhou, X., Lu, Y., Guo, P., & Zhou, C. (2021). Upregulation of microRNA‑140‑3p mediates dachshund family transcription factor 1 expression in immunoglobulin A nephropathy through cell cycle‑dependent mechanisms. Molecular medicine reports, 23(2), 1-1.

MacGrogan, D., & de la Pompa, J. L. (2021). DACH1-Driven Arterialization: Angiogenic Therapy for Ischemic Heart Disease?. Circulation Research, 129(7), 717-719.

Zhang, Y. L., Wang, J. M., Yin, H., Wang, S. B., He, C. L., & Liu, J. (2020). DACH1, a novel target of miR-218, participates in the regulation of cell viability, apoptosis, inflammatory response, and epithelial-mesenchymal transition process in renal tubule cells treated by high-glucose. Renal Failure, 42(1), 463-473.

Wang, R., Shen, J., Su, N., Wang, Q., Zhang, M., & Liu, C. (2020). MiR-645 regulates the proliferation and apoptosis of diffuse large B-cell lymphoma by targeting DACH1. Human cell, 33(4), 1091-1098.

Kim, Y. K., Lee, H., Ismail, T., Kim, Y., & Lee, H. S. (2020). Dach1 regulates neural crest migration during embryonic development. Biochemical and Biophysical Research Communications, 527(4), 896-901.

Cao, W., & Sun, J. (2019). MicroRNA-200c promotes tumor cell proliferation and migration by directly targeting dachshund family transcription factor 1 by the Wnt/β-catenin signaling pathway in nasopharyngeal carcinoma. Anti-Cancer Drugs, 30(3), 218-224.

Zhou, Q., Li, W., Kong, D., Liu, Z., Shi, Z., Ma, X., ... & Jiang, J. (2019). DACH1 suppresses epithelial to mesenchymal transition (EMT) through Notch1 pathway and reverses progestin resistance in endometrial carcinoma. Cancer medicine, 8(9), 4380-4388.

Cao, J., Yan, X. R., Liu, T., Han, X. B., Yu, J. J., Liu, S. H., & Wang, L. B. (2017). MicroRNA‑552 promotes tumor cell proliferation and migration by directly targeting DACH1 via the Wnt/β‑catenin signaling pathway in colorectal cancer. Oncology letters, 14(3), 3795-3802.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

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For research use only. Not intended for any clinical use.

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