Rabbit Anti-DAGLB Recombinant Antibody (D0183) (CBMAB-D0183-YC)

Name: Rabbit Anti-DAGLB Recombinant Antibody (D0183)
SKU: CBMAB-D0183-YC
Rating: 5 (1 reviews)

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Published Data

Rabbit Anti-DAGLB Recombinant Antibody (D0183) (CBMAB-D0183-YC) in IF

Human corneal epithelial cells express endocannabinoid receptors and endocannabinoid metabolizing enzymes in vitro: (A,B) CB1-specific, (C,D) CB2-specific, (E,F) NAPE-PLD-specific, (G,H) DAGLα-specific, (I,J) DAGLβ-specific, (K,L) FAAH-specific, and (M,N) MAGL-specific immunoreactivity, as shown by immunofluorescence (green fluorescence) on human corneal epithelial cell cultures. Nuclei were counterstained with DAPI (4',6-diamidino-2-phenylindole; blue fluorescence). (O) Negative control of panels C, D, G-N; (P) negative control of panels A, B, E, and F. Scale bars mark 100 μm or 50 μm, depending on magnification (200× and 400×, respectively). ...View More

Angyal, Á., Pénzes, Z., Alimohammadi, S., Horváth, D., Takács, L., Vereb, G., ... & Szöllősi, A. G. (2021). Anandamide concentration-dependently modulates toll-like receptor 3 agonism or UVB-induced inflammatory response of human corneal epithelial cells. International Journal of Molecular Sciences, 22(15), 7776. Distributed under Open Access license CC BY 4.0, without modification.

Rabbit Anti-DAGLB Recombinant Antibody (D0183) (CBMAB-D0183-YC) in IF

Epithelial cells of the human cornea express endocannabinoid receptors and endocannabinoid metabolizing enzymes in situ: (A) CB1-specific, (B) CB2-specific, (C) NAPE-PLD-specific, (D) DAGLα-specific, (E) DAGLβ-specific, (F) FAAH-specific, and (G) MAGL-specific immunoreactivity, as shown by immunofluorescence (red fluorescence) on human cornea sections. Nuclei were counterstained with DAPI (blue fluorescence). (H) negative control of panels B,D-G; (I) negative control of panels A,C. Scale bars mark 50 μm. ...View More

Datasheet

Summary

Host Animal

Mouse

Specificity

Human

Clone

D0183

Antibody Isotype

IgG1, λ

Application

WB, IP, IF, ELISA

Basic Information

Immunogen

Amino acids 554-569 within a C-terminal cytoplasmic domain of human DAGLβ.

Host Species

Mouse

Specificity

Human

Antibody Isotype

IgG1, λ

Clonality

Monoclonal Antibody

Application Notes

Application	Note
WB	1:100-1:1,000
IP	1-2 μg per 100-500 μg of total protein (1 mL of cell lysate)
ELISA	1:100-1:1,000
IF(ICC)	1:50-1:500

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS, 0.1% gelatin

Preservative

< 0.1% sodium azide

Concentration

0.2 mg/ml

Target

Full Name

Diacylglycerol Lipase Beta

Entrez Gene ID

Human	221955
Mouse	231871
Rat	304289
Monkey	717799

UniProt ID

Human	Q8NCG7
Mouse	Q91WC9
Rat	P0C1S9
Monkey	F6T9G9

Function

Lipase that catalyzes the hydrolysis of arachidonic acid (AA)-esterified diacylglycerols (DAGs) to produce the principal endocannabinoid, 2-arachidonoylglycerol (2-AG) which can be further cleaved by downstream enzymes to release arachidonic acid (AA) for cyclooxygenase (COX)-mediated eicosanoid production (PubMed:14610053).

Preferentially hydrolyzes DAGs at the sn-1 position in a calcium-dependent manner and has negligible activity against other lipids including monoacylglycerols and phospholipids (PubMed:14610053).

Plays a key role in the regulation of 2-AG and AA pools utilized by COX1/2 to generate lipid mediators of macrophage and microglia inflammatory responses. Functions also as a polyunsaturated fatty acids-specific triacylglycerol lipase in macrophages. Plays an important role to support the metabolic and signaling demands of macrophages (By similarity).

Biological Process

Arachidonic acid metabolic process Source: UniProtKB
Endocannabinoid signaling pathway Source: GO_Central
G protein-coupled glutamate receptor signaling pathway Source: GO_Central
Lipid catabolic process Source: UniProtKB-KW
Neuroblast proliferation Source: Ensembl
Neurogenesis Source: GO_Central
Neurotransmitter biosynthetic process Source: GO_Central
Positive regulation of triglyceride catabolic process Source: UniProtKB
Prostaglandin biosynthetic process Source: UniProtKB
Regulation of inflammatory response Source: UniProtKB
Retrograde trans-synaptic signaling by endocannabinoid Source: GO_Central

Cellular Location

Cell membrane

Topology

Cytoplasmic: 1-17
Helical: 18-38
Extracellular: 39-58
Helical: 59-79
Cytoplasmic: 80-102
Helical: 103-123
Extracellular: 124-132
Helical: 133-153
Cytoplasmic: 154-672

More Infomation

References

Liu, Z., Yang, N., Dong, J., Tian, W., Chang, L., Ma, J., ... & Tang, B. (2021). Deficiency in endocannabinoid synthase DAGLB contributes to Parkinson's disease and dopaminergic neuron dysfunction. bioRxiv.

Davies, A. K., Ziegler, M., Jumo, H., Saber, W. A., Ebrahimi-Fakhari, D., & Borner, G. H. (2020). AP-4 mediates vesicular transport of the 2-AG endocannabinoid producing enzyme DAGLB. BiorXiv.

Shin, M., Buckner, A., Prince, J., Bullock, T. N., & Hsu, K. L. (2019). Diacylglycerol lipase-β is required for TNF-α response but not CD8+ T cell priming capacity of dendritic cells. Cell chemical biology, 26(7), 1036-1041.

Zhou, D., Zhang, D., Sun, X., Li, Z., Ni, Y., Shan, Z., ... & Lai, M. (2018). A novel variant associated with HDL-C levels by modifying DAGLB expression levels: An annotation-based genome-wide association study. European Journal of Human Genetics, 26(6), 838-847.

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Protocols

Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
Other Protocols

Associated Products

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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Documents

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SDS
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Second antibody and isotype control

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