Mouse Anti-DAPP1 Recombinant Antibody (4H9) (V2LY-0125-LY1109)

Name: Mouse Anti-DAPP1 Recombinant Antibody (4H9)
SKU: V2LY-0125-LY1109
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

4H9

Application

IP, WB, ELISA

Immunogen

Recombinant mouse BAM32.

Host Species

Mouse

Specificity

Human, Mouse, Rat

Antibody Isotype

IgG1, κ

Clonality

Monoclonal Antibody

Application Notes

Application	Note
WB	1:100-1:1,000
IP	1-2 μg per 100-500 μg of total protein (1 mL of cell lysate)
ELISA	1:100-1:1,000

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

Gelatin & PBS

Preservative

Sodium Azide

Concentration

0.2 mg/mL

Purity

>95% as determined by analysis by SDS-PAGE

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

More Infomation

Target

Full Name

Dual Adaptor Of Phosphotyrosine And 3-Phosphoinositides 1

Entrez Gene ID

Human	27071
Mouse	26377

UniProt ID

Human	Q9UN19
Mouse	Q9QXT1

Function

May act as a B-cell-associated adapter that regulates B-cell antigen receptor (BCR)-signaling downstream of PI3K.

Biological Process

Protein dephosphorylation Source: UniProtKB
Signal transduction Source: ProtInc

Cellular Location

Cytoplasm; Membrane. Membrane-associated after cell stimulation leading to its translocation.

PTM

Phosphorylated on tyrosine residues.

Chen, S., Qi, Y., Wang, S., Xu, Y., Shen, M., Hu, M., ... & Wang, J. (2020). Melatonin enhances thrombopoiesis through ERK1/2 and Akt activation orchestrated by dual adaptor for phosphotyrosine and 3‐phosphoinositides. Journal of Pineal Research, 68(3), e12637.

Hao, L., Marshall, A. J., & Liu, L. (2020). Bam32/DAPP1-dependent neutrophil reactive oxygen species in WKYMVm-induced microvascular hyperpermeability. Frontiers in Immunology, 11, 1028.

Durrant, T. N., Hutchinson, J. L., Heesom, K. J., Anderson, K. E., Stephens, L. R., Hawkins, P. T., ... & Hers, I. (2017). In-depth PtdIns (3, 4, 5) P3 signalosome analysis identifies DAPP1 as a negative regulator of GPVI-driven platelet function. Blood advances, 1(14), 918-932.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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