Mouse Anti-DCSTAMP Recombinant Antibody (CBYCD-184) (V2LY-0125-LY1232)

Name: Mouse Anti-DCSTAMP Recombinant Antibody (CBYCD-184)
SKU: V2LY-0125-LY1232
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

CBYCD-184

Application

Immunogen

E. coli-derived recombinant human DC-STAMP, Asp314-Thr376.

Host Species

Mouse

Specificity

Human

Antibody Isotype

IgG2b

Clonality

Monoclonal Antibody

Application Notes

Application	Note
FC	2.5 μg/10⁶ cells

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Lyophilized

Buffer

PBS & Trehalose

Preservative

None

Purity

>95% as determined by analysis by SDS-PAGE

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

More Infomation

Target

Full Name

Dendrocyte Expressed Seven Transmembrane Protein

Entrez Gene ID

81501

UniProt ID

Q9H295

Function

Probable cell surface receptor that plays several roles in cellular fusion, cell differentiation, bone and immune homeostasis. Plays a role in TNFSF11-mediated osteoclastogenesis. Cooperates with OCSTAMP in modulating cell-cell fusion in both osteoclasts and foreign body giant cells (FBGCs). Participates in osteoclast bone resorption. Involved in inducing the expression of tartrate-resistant acid phosphatase in osteoclast precursors. Plays a role in haematopoietic stem cell differentiation of bone marrow cells toward the myeloid lineage. Inhibits the development of neutrophilic granulocytes. Plays also a role in the regulation of dendritic cell (DC) antigen presentation activity by controlling phagocytic activity. Involved in the maintenance of immune self-tolerance and avoidance of autoimmune reactions.

Biological Process

Cellular response to interleukin-4 Source: UniProtKB
Cellular response to macrophage colony-stimulating factor stimulus Source: UniProtKB
Cellular response to tumor necrosis factor Source: UniProtKB
Membrane fusion Source: UniProtKB
Myeloid dendritic cell differentiation Source: UniProtKB
Negative regulation of cell growth Source: UniProtKB
Osteoclast differentiation Source: UniProtKB
Osteoclast fusion Source: UniProtKB
Positive regulation of bone resorption Source: UniProtKB
Positive regulation of macrophage fusion Source: UniProtKB
Positive regulation of monocyte differentiation Source: UniProtKB

Cellular Location

Endosome; Endoplasmic reticulum membrane; Cell membrane; Endoplasmic reticulum-Golgi intermediate compartment membrane. Localizes to the cell surface in osteoclasts and undifferentiated monocytes. Intracellular internalized DCSTAMP is detected in a fraction of RANKL-induced osteoclast precursor. Colocalizes with OS9 in the endoplasmic reticulum (ER) of immature dendritic cell (DC). Translocates from the endoplasmic reticulum to the intermediate/Golgi compartment upon maturation of DC in a OS9-dependent manner. Colocalizes with LAMP1 in endosomes (By similarity).

Topology

Cytoplasmic: 1-34
Helical: 35-55
Extracellular: 56-57
Helical: 58-78
Cytoplasmic: 79-97
Helical: 98-118
Extracellular: 119-209
Helical: 210-230
Cytoplasmic: 231-292
Helical: 293-313
Extracellular: 314-376
Helical: 377-397
Cytoplasmic: 398-470

PTM

Glycosylated.

Zou, B. H., Tan, Y. H., Deng, W. D., Zheng, J. H., Yang, Q., Ke, M. H., ... & Li, X. J. (2021). Oridonin ameliorates inflammation-induced bone loss in mice via suppressing DC-STAMP expression. Acta Pharmacologica Sinica, 42(5), 744-754.

Guardiola, C. J. D. A., Clemente-Napimoga, J. T., Martinez, E. F., Abdalla, H. B., Peruzzo, D. C., Joly, J. C., & Napimoga, M. H. (2020). DC-STAMP and TACE Levels are Higher in Patients with Periodontitis. Brazilian Dental Journal, 31, 122-126.

Møller, A. M., Delaissé, J. M., Olesen, J. B., Canto, L. M., Rogatto, S. R., Madsen, J. S., & Søe, K. (2020). Fusion potential of human osteoclasts in vitro reflects age, menopause, and in vivo bone resorption levels of their donors—a possible involvement of DC-STAMP. International journal of molecular sciences, 21(17), 6368.

Kuriya, K., Nishio, M., Matsuda, T., & Umekawa, H. (2020). Tea extract increases cell fusion via regulation of cell surface DC-STAMP. Biochemistry and Biophysics Reports, 22, 100759.

Mullin, B. H., Zhu, K., Brown, S. J., Mullin, S., Tickner, J., Pavlos, N. J., ... & Wilson, S. G. (2019). Genetic regulatory mechanisms in human osteoclasts suggest a role for the STMP1 and DCSTAMP genes in Paget’s disease of bone. Scientific reports, 9(1), 1-7.

Takagi, T., Inoue, H., Takahashi, N., Katsumata-Tsuboi, R., & Uehara, M. (2017). Sulforaphane inhibits osteoclast differentiation by suppressing the cell-cell fusion molecules DC-STAMP and OC-STAMP. Biochemical and biophysical research communications, 483(1), 718-724.

Yin, Y., Tang, L., Chen, J., & Lu, X. (2017). MiR-30a attenuates osteoclastogenesis via targeting DC-STAMP-c-Fos-NFATc1 signaling. American Journal of Translational Research, 9(12), 5743.

Chiu, Y. H., Schwarz, E., Li, D., Xu, Y., Sheu, T. R., Li, J., ... & Ritchlin, C. T. (2017). Dendritic cell‐specific transmembrane protein (DC‐STAMP) regulates osteoclast differentiation via the Ca2+/NFATc1 axis. Journal of cellular physiology, 232(9), 2538-2549.

Wisitrasameewong, W., Kajiya, M., Movila, A., Rittling, S., Ishii, T., Suzuki, M., ... & Kawai, T. (2017). DC-STAMP is an osteoclast fusogen engaged in periodontal bone resorption. Journal of dental research, 96(6), 685-693.

Takagi, T., Inoue, H., Takahashi, N., Katsumata-Tsuboi, R., & Uehara, M. (2017). Sulforaphene attenuates multinucleation of pre-osteoclasts by suppressing expression of cell–cell fusion-associated genes DC-STAMP, OC-STAMP, and Atp6v0d2. Bioscience, Biotechnology, and Biochemistry, 81(6), 1220-1223.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
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Isotype control

For research use only. Not intended for any clinical use.

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