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Mouse Anti-DCTN4 Recombinant Antibody (CBYCD-189) (V2LY-0125-LY1254)


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Published Data
V2LY-0125-LY1254-1
Mouse Anti-DCTN4 Recombinant Antibody (CBYCD-189) (V2LY-0125-LY1254) in IF
Co-localization of proteasome 20 s aggregates and HDAC6 after aldosterone administration.
A) Double fluorescence labeling for RPL22 aggregates (H-95, red) and HDAC6 (green) in kidney from control rat. B) Similar labeling in kidney from aldosterone treated rat. Arrow heads indicate colocalization (in yellow). C) The immunostaing for the de-ubiquitinase ataxin-3 in control rat kidney sections. D) Representative micrograph of renal cortex of an aldosterone treated rat stained for ataxin-3. E) Double fluorescence labeling for aggresome initiator protein p62 (red) and HDAC6 (green) in kidney from control rat. F) Similar labeling in kidney from aldosterone treated rat. G) Renal cortex from control rats was labeled for autophagosomes by anti-LC3 antibodies (green) and overlaid on DIC image. H) Similar fluorescence labeling of renal cortex from aldosterone treated rat. I) Similar staining where distal renal tubules were identified by calbindin-D28K (red). J) Corresponding micrograph from aldosterone treated rat. ...View More
Cheema, M. U., Damkier, H. H., Nielsen, J., Poulsen, E. T., Enghild, J. J., Fenton, R. A., & Praetorius, J. (2014). Distal renal tubules are deficient in aggresome formation and autophagy upon aldosterone administration. PLoS One, 9(7), e101258. Distributed under Open Access license CC BY 4.0, without modification.
V2LY-0125-LY1254-2
Mouse Anti-DCTN4 Recombinant Antibody (CBYCD-189) (V2LY-0125-LY1254) in IHC
Renal cortex immunoperoxidase staining pattern for dynactin p62, which is a linker protein between cargo bound to HDAC6 destined for aggresomes and the motor protein dynein. ...View More
Cheema, M. U., Poulsen, E. T., Enghild, J. J., Hoorn, E., Fenton, R. A., & Praetorius, J. (2013). Aldosterone and angiotensin II induce protein aggregation in renal proximal tubules. Physiological Reports, 1(4). Distributed under Open Access license CC BY 3.0, without modification.
V2LY-0125-LY1254-3
Mouse Anti-DCTN4 Recombinant Antibody (CBYCD-189) (V2LY-0125-LY1254) in IF
Representative micrograph of dynactin p62 (red) and HDAC6 (green) colabeling. ...View More
Cheema, M. U., Poulsen, E. T., Enghild, J. J., Hoorn, E., Fenton, R. A., & Praetorius, J. (2013). Aldosterone and angiotensin II induce protein aggregation in renal proximal tubules. Physiological Reports, 1(4). Distributed under Open Access license CC BY 3.0, without modification.

Summary

Host Animal
Mouse
Specificity
Human, Mouse, Rat
Clone
CBYCD-189
Antibody Isotype
IgG2a, κ
Application
WB, IP, IF, ELISA, IHC-P

Basic Information

Immunogen
Amino acids 161-460 of human Dynactin p62.
Host Species
Mouse
Specificity
Human, Mouse, Rat
Antibody Isotype
IgG2a, κ
Clonality
Monoclonal Antibody
Application Notes
ApplicationNote
WB1:100-1:1,000
IP1-2 μg per 100-500 μg of total protein (1 mL of cell lysate)
IF(ICC)1:50-1:500
ELISA1:100-1:1,000
IHC-P1:50-1:500

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
Gelatin & PBS
Preservative
Sodium Azide
Concentration
0.2 mg/mL
Purity
>95% as determined by analysis by SDS-PAGE
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Target

Full Name
dynactin 4
Entrez Gene ID
Human51164
Mouse67665
Rat84428
UniProt ID
HumanQ9UJW0
MouseQ8CBY8
RatQ9QUR2
Function
Could have a dual role in dynein targeting and in ACTR1A/Arp1 subunit of dynactin pointed-end capping. Could be involved in ACTR1A pointed-end binding and in additional roles in linking dynein and dynactin to the cortical cytoskeleton.
Biological Process
Antigen processing and presentation of exogenous peptide antigen via MHC class II Source: Reactome
Endoplasmic reticulum to Golgi vesicle-mediated transport Source: Reactome
Cellular Location
Cytoskeleton; Centrosome; Stress fiber; Cell cortex; sarcomere. Has a punctate cytoplasmic distribution as well as centrosomal distribution typical of dynactin (PubMed:10671518). Overexpression in cultured mammalian cells revealed colocalization with cortical actin, stress fibers, and focal adhesion sites, sites of potential interaction between microtubules and the cell cortex (By similarity). In skeletal muscles, costamere localization requires the presence of ANK2 (By similarity).
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For research use only. Not intended for any clinical use.

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