Mouse Anti-DCTN4 Recombinant Antibody (CBYCD-189) (V2LY-0125-LY1254)

Name: Mouse Anti-DCTN4 Recombinant Antibody (CBYCD-189)
SKU: V2LY-0125-LY1254
Rating: 5 (1 reviews)

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Published Data

Mouse Anti-DCTN4 Recombinant Antibody (CBYCD-189) (V2LY-0125-LY1254) in IF

Co-localization of proteasome 20 s aggregates and HDAC6 after aldosterone administration.
A) Double fluorescence labeling for RPL22 aggregates (H-95, red) and HDAC6 (green) in kidney from control rat. B) Similar labeling in kidney from aldosterone treated rat. Arrow heads indicate colocalization (in yellow). C) The immunostaing for the de-ubiquitinase ataxin-3 in control rat kidney sections. D) Representative micrograph of renal cortex of an aldosterone treated rat stained for ataxin-3. E) Double fluorescence labeling for aggresome initiator protein p62 (red) and HDAC6 (green) in kidney from control rat. F) Similar labeling in kidney from aldosterone treated rat. G) Renal cortex from control rats was labeled for autophagosomes by anti-LC3 antibodies (green) and overlaid on DIC image. H) Similar fluorescence labeling of renal cortex from aldosterone treated rat. I) Similar staining where distal renal tubules were identified by calbindin-D28K (red). J) Corresponding micrograph from aldosterone treated rat. ...View More

Cheema, M. U., Damkier, H. H., Nielsen, J., Poulsen, E. T., Enghild, J. J., Fenton, R. A., & Praetorius, J. (2014). Distal renal tubules are deficient in aggresome formation and autophagy upon aldosterone administration. PLoS One, 9(7), e101258. Distributed under Open Access license CC BY 4.0, without modification.

Mouse Anti-DCTN4 Recombinant Antibody (CBYCD-189) (V2LY-0125-LY1254) in IHC

Renal cortex immunoperoxidase staining pattern for dynactin p62, which is a linker protein between cargo bound to HDAC6 destined for aggresomes and the motor protein dynein. ...View More

Cheema, M. U., Poulsen, E. T., Enghild, J. J., Hoorn, E., Fenton, R. A., & Praetorius, J. (2013). Aldosterone and angiotensin II induce protein aggregation in renal proximal tubules. Physiological Reports, 1(4). Distributed under Open Access license CC BY 3.0, without modification.

Mouse Anti-DCTN4 Recombinant Antibody (CBYCD-189) (V2LY-0125-LY1254) in IF

Representative micrograph of dynactin p62 (red) and HDAC6 (green) colabeling. ...View More

Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

CBYCD-189

Application

WB, IP, IF, ELISA, IHC-P

Immunogen

Amino acids 161-460 of human Dynactin p62.

Host Species

Mouse

Specificity

Human, Mouse, Rat

Antibody Isotype

IgG2a, κ

Clonality

Monoclonal Antibody

Application Notes

Application	Note
WB	1:100-1:1,000
IP	1-2 μg per 100-500 μg of total protein (1 mL of cell lysate)
IF(ICC)	1:50-1:500
ELISA	1:100-1:1,000
IHC-P	1:50-1:500

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

Gelatin & PBS

Preservative

Sodium Azide

Concentration

0.2 mg/mL

Purity

>95% as determined by analysis by SDS-PAGE

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

More Infomation

Target

Full Name

dynactin 4

Entrez Gene ID

Human	51164
Mouse	67665
Rat	84428

UniProt ID

Human	Q9UJW0
Mouse	Q8CBY8
Rat	Q9QUR2

Function

Could have a dual role in dynein targeting and in ACTR1A/Arp1 subunit of dynactin pointed-end capping. Could be involved in ACTR1A pointed-end binding and in additional roles in linking dynein and dynactin to the cortical cytoskeleton.

Biological Process

Antigen processing and presentation of exogenous peptide antigen via MHC class II Source: Reactome
Endoplasmic reticulum to Golgi vesicle-mediated transport Source: Reactome

Cellular Location

Cytoskeleton; Centrosome; Stress fiber; Cell cortex; sarcomere. Has a punctate cytoplasmic distribution as well as centrosomal distribution typical of dynactin (PubMed:10671518). Overexpression in cultured mammalian cells revealed colocalization with cortical actin, stress fibers, and focal adhesion sites, sites of potential interaction between microtubules and the cell cortex (By similarity). In skeletal muscles, costamere localization requires the presence of ANK2 (By similarity).

Su, X., Li, H., Chen, S., & Qin, C. (2021). Study on the prognostic values of dynactin genes in low-grade glioma. Technology in Cancer Research & Treatment, 20, 15330338211010143.

Wang, S., Wang, Q., Zhang, X., Liao, X., Wang, G., Yu, L., ... & Yuan, W. (2018). Distinct prognostic value of dynactin subunit 4 (DCTN4) and diagnostic value of DCTN1, DCTN2, and DCTN4 in colon adenocarcinoma. Cancer Management and Research, 10, 5807.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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