Mouse Anti-DDAH2 Recombinant Antibody (3E3) (CBMAB-D0446-YC)

Provided herein is a Mouse monoclonal antibody, which binds to Dimethylarginine Dimethylaminohydrolase 2 (DDAH2). The antibody can be used for immunoassay techniques, such as ELISA, WB.
See all DDAH2 antibodies

Published Data

Fig.1 Recombinant expression of human DDAH II.

Datasheet

Summary

Host Animal

Mouse

Specificity

Human

Clone

3E3

Antibody Isotype

IgG2b, κ

Application

ELISA, WB

Basic Information

Immunogen

DDAH2 (AAH01435, 1 a.a. ~ 285 a.a) full-length recombinant protein with GST tag.

Specificity

Human

Antibody Isotype

IgG2b, κ

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Storage

Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name

dimethylarginine dimethylaminohydrolase 2

Introduction

DDAH2 is a dimethylarginine dimethylaminohydrolase. The encoded enzyme functions in nitric oxide generation by regulating the cellular concentrations of methylarginines, which in turn inhibit nitric oxide synthase activity. The protein may be localized to the mitochondria. Alternative splicing resulting in multiple transcript variants.

Entrez Gene ID

23564

UniProt ID

O95865

Alternative Names

Dimethylarginine Dimethylaminohydrolase 2; Dimethylargininase-2; S-Phase Protein; EC 3.5.3.18; DDAHII; DDAH; NG30; G6a;

Function

Hydrolyzes N(G),N(G)-dimethyl-L-arginine (ADMA) and N(G)-monomethyl-L-arginine (MMA) which act as inhibitors of NOS. Has therefore a role in the regulation of nitric oxide generation.

Biological Process

Arginine catabolic process Source: ProtInc
Arginine metabolic process Source: GO_Central
Citrulline metabolic process Source: UniProtKB
Negative regulation of apoptotic process Source: UniProtKB
Nitric oxide biosynthetic process Source: ProtInc
Nitric oxide mediated signal transduction Source: ProtInc
Positive regulation of nitric oxide biosynthetic process Source: BHF-UCL
Regulation of nitric-oxide synthase activity Source: Reactome

Cellular Location

Cytoplasm; Mitochondrion. Translocates from cytosol to mitochondrion upon IL-1beta stimulation in chondrocytes.

References

Chen, W., Wang, H., Liu, J., & Li, K. (2022). Interference of KLF9 relieved the development of gestational diabetes mellitus by upregulating DDAH2. Bioengineered, 13(1), 395-406.

Li, J., Sun, L., & Li, Y. (2021). Regulation of dimethylarginine dimethylaminohydrolase 2 expression by NF‑κB acetylation. Experimental and Therapeutic Medicine, 21(2), 1-1.

Lin, H. H., Lee, T. S., Lin, S. J., Yeh, Y. C., Lu, T. M., & Hsu, C. P. (2019). DDAH-2 alleviates contrast medium iopromide-induced acute kidney injury through nitric oxide synthase. Clinical Science, 133(23), 2361-2378.

Mannino, G. C., Pezzilli, S., Averta, C., Fuoco, A., Spiga, R., Mancuso, E., ... & Sesti, G. (2019). A functional variant of the dimethylarginine dimethylaminohydrolase-2 gene is associated with myocardial infarction in type 2 diabetic patients. Cardiovascular Diabetology, 18(1), 1-9.

Zhu, Z. D., Ye, J. M., Fu, X. M., Wang, X. C., Ye, J. Y., Wu, X. R., ... & Zhang, X. (2019). DDAH2 alleviates myocardial fibrosis in diabetic cardiomyopathy through activation of the DDAH/ADMA/NOS/NO pathway in rats. International Journal of Molecular Medicine, 43(2), 749-760.

Lambden, S., Tomlinson, J., Piper, S., Gordon, A. C., & Leiper, J. (2018). Evidence for a protective role for the rs805305 single nucleotide polymorphism of dimethylarginine dimethylaminohydrolase 2 (DDAH2) in septic shock through the regulation of DDAH activity. Critical Care, 22(1), 1-9.

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Protocols

Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Associated Products

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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